Assignment and secondary structure of the YadA membrane protein by solid-state MAS NMR

Shahid, Shakeel Ahmad; Marković, Stefan; Linke, Dirk; Rossum, Barth‐Jan van

doi:10.1038/srep00803

Cited by 26 publications

(16 citation statements)

References 36 publications

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“…When conditions are suitable, cells strengthen their attachment and divide to form a growing population on the surface (O’Toole et al 2000). Attachment is facilitated by a range of secreted products, including polysaccharides, proteins, nucleic acids, and amyloids (Absalon et al 2011, Garcia et al 2011, Linke et al 2006, Ma et al 2006, Romero et al 2010, Shahid, Bardiaux, Franks, Krabben, Habeck, van Rossum & Linke 2012, Shahid, Markovic, Linke & van Rossum 2012, Timmerman et al 1991, Veenstra et al 1996). …”

Section: Introductionmentioning

confidence: 99%

Adhesion as a weapon in microbial competition

et al. 2014

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Microbes attach to surfaces and form dense communities known as biofilms, which are central to how microbes live and influence humans. The key defining feature of biofilms is adhesion, whereby cells attach to one another and to surfaces, via attachment factors and extracellular polymers. While adhesion is known to be important for the initial stages of biofilm formation, its function within biofilm communities has not been studied. Here we utilise an individual-based model of microbial groups to study the evolution of adhesion. While adhering to a surface can enable cells to remain in a biofilm, consideration of within-biofilm competition reveals a potential cost to adhesion: immobility. Highly adhesive cells that are resistant to movement risk being buried and starved at the base of the biofilm. However, we find that when growth occurs at the base of a biofilm, adhesion allows cells to capture substratum territory and force less adhesive, competing cells out of the system. This process may be particularly important when cells grow on a host epithelial surface. We test the predictions of our model using the enteric pathogen Vibrio cholerae, which produces an extracellular matrix important for biofilm formation. Flow cell experiments indicate that matrix-secreting cells are highly adhesive and form expanding clusters that remove non-secreting cells from the population, as predicted by our simulations. Our study shows how simple physical properties, such as adhesion, can be critical to understanding evolution and competition within microbial communities.

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Section: Introductionmentioning

confidence: 99%

Adhesion as a weapon in microbial competition

et al. 2014

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“…High quality solid-state NMR spectra have been reported for samples of membrane proteins in proteolipid 2D crystals, precipitates, and microcrystals (Andreas et al 2015; Barbet-Massin et al 2014; Eddy et al 2012; Li et al 2007; Linser et al 2011; Saurel et al 2017; Shahid et al 2012). These samples, prepared with lipid to protein molecular ratios in the range of 6:1 and 25:1, have the advantage that large quantities of protein can be packed in the NMR rotor for enhanced sensitivity.…”

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confidence: 99%

High resolution solid-state NMR spectroscopy of the Yersinia pestis outer membrane protein Ail in lipid membranes

et al. 2017

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The outer membrane protein Ail (Adhesion invasion locus) is one of the most abundant proteins on the cell surface of Yersinia pestis during human infection. Its functions are expressed through interactions with a variety of human host proteins, and are essential for microbial virulence. Structures of Ail have been determined by X-ray diffraction and solution NMR spectroscopy, but those samples contained detergents that interfere with functionality, thus, precluding analysis of the structural basis for Ail’s biological activity. Here, we demonstrate that high-resolution solid-state NMR spectra can be obtained from samples of Ail in detergent-free phospholipid liposomes, prepared with a lipid to protein molar ratio of 100. The spectra, obtained with 13C or 1H detection, have very narrow line widths (0.40–0.60 ppm for 13C, 0.11–0.15 ppm for 1H, and 0.46–0.64 ppm for 15N) that are consistent with a high level of sample homogeneity. The spectra enable resonance assignments to be obtained for N, CO, CA and CB atomic sites from 75 out of 156 residues in the sequence of Ail, including 80% of the transmembrane region. The 1H-detected solid-state NMR 1H/15N correlation spectra obtained for Ail in liposomes compare very favorably with the solution NMR 1H/15N TROSY spectra obtained for Ail in nanodiscs prepared with a similar lipid to protein molar ratio. These results set the stage for studies of the molecular basis of the functional interactions of Ail with its protein partners from human host cells, as well as the development of drugs targeting Ail.

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“…These pores act as channels for transporting the three N-terminal a-helices passenger domains through the bacterial outer membrane (Roggenkamp et al 2003;Oomen et al 2004;Surana et al 2004;Meng et al 2006). Shahid et al predicted the secondary sturcture of YadA-M by taking advantage of solid-state magic-angle spinning (MAS) NMR and indicated that this domain contains a number of amphipathic, antiparallel b-strands, at both ends of these b-strands are frequently made from aromatic residues that interact with the water-lipid interface to anchor the TAAs in the lipid membrane (Shahid et al 2012). …”

Section: The Common Molecular Structures Of Taasmentioning

confidence: 98%

New findings on the function and potential applications of the trimeric autotransporter adhesin

Qin

Wang

Lei

2015

Antonie van Leeuwenhoek

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Trimeric autotransporter adhesins (TAAs) are located on the surface of many pathogenic Gram-negative bacteria. TAAs belong to the autotransporter protein family and consist of three identical monomers. These obligate homotrimeric proteins are secreted through the bacterial type Vc secretion system and share a common molecular organization that each monomer consists of a N-terminal "passenger" domain and a C-terminal translocation domain. TAAs are important virulence factors that are involved in bacterial life cycle and participate in mediating infection, invasion, dissemination and evasion of host immune responses. TAAs have also proved to be useful for many applications, such as vaccines and disease biomarkers. We here mainly focused on new findings on bio-function and application of TAAs in addition to their common structure and secretion mechanisms.

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Assignment and secondary structure of the YadA membrane protein by solid-state MAS NMR

Cited by 26 publications

References 36 publications

Adhesion as a weapon in microbial competition

Adhesion as a weapon in microbial competition

High resolution solid-state NMR spectroscopy of the Yersinia pestis outer membrane protein Ail in lipid membranes

New findings on the function and potential applications of the trimeric autotransporter adhesin

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