Assignment of parentage by microsatellite analysis in the endangered Brachymystax lenok tsinlingensis (Salmonidae)

Abstract

We evaluated the use of microsatellite markers to identify parentage in Brachy mystax lenok tsinlingensis Li, 1966, an endangered salmonid. Computer simulations showed that when no parent information was available, or when information was only available from 1 parent, then exclusion probabilities ranged from 23.6 to 45.1% and from 25.9 to 52.4%, respectively; combined exclusion probabilities for the 8 loci investigated were 97.7 and 98.4%, respectively. However, a breeding experiment with known parental and filial information resulted in 91.28% of progeny being exclusively assigned to their correct parent pair. Our data demonstrate that microsatellitebased parentage assignment is a reliable means with which to obtain information pertaining to genealogical relationships and could therefore benefit genetic conservation of this species.

Genetic evidence for the mating system and reproductive success of black sea bream (Acanthopagrus schlegelii)

“…Microsatellite genotyping errors occur approximately 2%–3% per locus, and therefore, it was unlikely that determining parentage with 100% accuracy would occur in practice (O'Reilly, Herbinger, & Wright, 1998). Microsatellites have been commonly used for parentage analysis in a range of aquaculture species, while the accuracy has varied significantly across studies (Liu et al, 2012; Pruett et al, 2010; Shao et al, 2017; Yang et al, 2014). In a study of common carp ( Cyprinus carpio L.), the assignment success rate for 550 offspring from a full factorial cross of 10 dams × 24 sires with 8 microsatellite markers was 95.3% (Vandeputte et al, 2004).…”

Development and Characterization of Fifteen Polymorphic Microsatellite Loci for Rare and Endangered Species within Luciobarbus Heckel, 1843 Genus in the Aral Basin and Their Conservation Application

“…Of the 17 analyzed loci, 15 were polymorphic for all individuals; microsatellites were used to study the genetic structure and diversity of both L. brachycephalus and L. conocephalus. The number of alleles per locus is estimated to range from 2 to 12, namely M2044 and M4474-12, M1287-11, M4215-9, and M3444-8, with an average value of 6, in comparison with the number of alleles that fluctuate from 2 to 7 in other species [51][52][53][54]. Additionally, at two points where L. brachycephalus is usually recorded, four individuals of L. conocephalus were also found, which was confirmed by our genetic results.…”