Assignment of the 13c nmr spectra of fusidic acid derivatives. Biosynthetic incorporation of sodium [1-13c]-acetate into fusidic acid

On the absence of the glyceraldehyde 3-phosphate/pyruvate pathway for isoprenoid biosynthesis in fungi and yeasts

“…Radiolabeled MVA was incorporated by Gibberella fujikuroi into kaurene and gibberellins [20]. Such conclusions drawn from radiolabeling were con¢rmed by incorporation of IQ C-labeled acetate, for instance, into the hemiterpenic moiety of tajixanthone from Aspergillus variecolor [21], the sesquiterpenes helicobasidin from Helicobasidium mompa [22] and PR toxin from Penicillium roqueforti [23], the sesquiterpenic moiety of polyketide-terpenoid metabolites, such as andibenin from Aspergillus variecolor [24], austin from Aspergillus ustus [25] or sorokinianin from Bipolaris sorokiniana [26], the diterpenoid aphidicolin from Cephalosporium aphidicola [27] and ¢nally triterpenoids, such as fusidic acid from Fusidium coccineum [28] and ergosterol in yeast [29].…”

Reassignment of the 1H NMR spectrum of fusidic acid and total assignment of 1H and 13C NMR spectra of some selected fusidane derivatives

“…Only 13 C and crude 1 H NMR data of 1 obtained by selective 13 C-1 H spin decoupling 4 have previously been published.…”

Full assignments of the 1H and 13C NMR spectra of sodium fusidate in organic and aqueous media

“…The "C peak at 6 49.9 showed coupling to the same methyl singlets and also to H-16 (6 5.77) and H-12eq (6 2.28). This I3C signal was therefore assigned to (2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14) and the signal at 6 40.6 to C.8. The I3C signal at 6 51.26, due to C-9, showed long-range coupling to H-12eq (6 2.28), H,-19 (6 0.99) and the methyl signal at 6 1.38, which was therefore assigned to H,-32.…”