Assignments of the human genes for lactate dehydrogenase-A and thymidine kinase to specific chromosomal regions

Francke, Uta; Busby, N

doi:10.1159/000130371

Cited by 21 publications

(7 citation statements)

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“…Because LDH-A has been assigned to the short arm of chromosome 11 (31), we suggest that these two genes may be closely linked on the short arm of this chromosome. Recent results from other laboratories (32,33) (34), reported the activation of an a-globin gene derived from a mouse teratocarcinoma cell after fusion of a teratocarcinoma cell with MEL cells.…”

Section: Cell Fusion Of Mouse Erythroleukemia and Humanmentioning

confidence: 89%

Activation of human beta-globin genes from nonerythroid cells by fusion with murine erythroleukemia cells.

Pyati

Kucherlapati

Skoultchi³

1980

Proc. Natl. Acad. Sci. U.S.A.

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A human beta-globin gene derived from an established human lymphoblast cell line was introduced into murine erythroleukemia (MEL) cells by cell fusion. The globin genes in MEL cells are inducible by dimethyl sulfoxide (Me2SO); induction leads to the accumulation of mouse globin mRNA and hemoglobin. Globin mRNA was not detected in the cytoplasm of the human lymphoblast cells, even at low levels, whether or not these cells were treated with Me2SO. In cell hybrids that had retained the lymphoblast-derived beta-globin gene, human beta-globin mRNA was induced by Me2SO. Poly(A)-containing 10S human beta-globin mRNA was detected in the cytoplasm of the hybrid cells. Karyologic and isozymic analyses of a series of hybrids and subclones showed that human beta-globin gene expression occurred only in hybrids that had retained human chromosome 11. Analysis of one hybrid bearing a deletion of both the beta-globin and lactate dehydrogenase A genes indicated that the beta-globin gene is located on the short arm of human chromosome 11. No other human chromosomes are required for human beta-globin gene expression in MEL cell hybrids. We conclude that the restricted expression of a globin gene in a human nonerythroid cell can be reversed. Furthermore, all components required for the transcription, processing, and transport to the cytoplasm of a human globin mRNA appear to be present in mouse erythroleukemia cells. Thus cell fusion with MEL cells provides a way to isolate permanent cell lines with functioning human globin genes. The technique should be useful for studying the biochemical basis for abnormal function of mutant globin genes, such as those present in individuals with the thalassemia syndromes.

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Section: Cell Fusion Of Mouse Erythroleukemia and Humanmentioning

confidence: 89%

Activation of human beta-globin genes from nonerythroid cells by fusion with murine erythroleukemia cells.

Pyati

Kucherlapati

Skoultchi³

1980

Proc. Natl. Acad. Sci. U.S.A.

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“…A gene for lysosomal acid phosphatase (ACP-2) has been assigned to the short arm of chromosome 11 (Bruns and G erald, 1974;Busby et al. 1975).…”

Section: Discussionmentioning

confidence: 99%

Gene dose effect: intraband mapping of the <i>LDH A </i>locus using cells from four individuals with different interstitial deletions of 11p

Francke¹,

George²,

Brown³

et al. 1977

Cytogenet Genome Res

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“…15 Gene Mapping Conference in Rotterdam in 1974 to present our initial work. 22,23 At these biannual, or later annual, conferences supported by the March of Dimes Birth Defects Foundation, researchers from all over the world came together to share their latest physical and genetic-mapping data, which were then integrated and compiled by chromosome-specific committees. The updated mapping reports were published in Cytogenetics and Cell Genetics.…”

Section: Mapping Genes Onto Chromosomal Regionsmentioning

confidence: 99%

“…Initially, the phenotypes we could map in somatic cell hybrids were limited to expressed proteins for which the human-specific forms could be distinguished from the rodent forms, e.g., metabolic enzymes, [22][23][24] cell-surface antigens, such as human leukocyte antigen, 25 polypeptide spots on two-dimensional protein gels, 26 or yet-unidentified factors responsible for virus replication in cultured human cells. 27 In the early 1980s, with the advent of molecular probes, we were able to map hybridizing restriction fragments on Southern blots made with DNA from interspecies somaticcell-hybrid clones.…”

Section: Mapping Genes Onto Chromosomal Regionsmentioning

confidence: 99%

2012 William Allan Award: Adventures in Cytogenetics1

Francke

2013

The American Journal of Human Genetics

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Ladies and gentlemen, members and guests, colleagues and friends, I am deeply honored to be chosen for this award; thank you, Madam President, members of the Awards Committee, and Tayfun Ö zçelik for your kind introduction. I accept this award on behalf of a large group of scientists, numerous postdocs, graduate and undergraduate students, research associates, and technicians. I am grateful to all of them, some of whom are here, and to the many collaborators who contributed to our success.You heard about the wide range of research activities of my laboratory, so for this address, I had to select what to cover. The microscope in the Allan Award medal inspired me to focus on my adventures in cytogenetics.Let's start from the beginning. In the gymnasium, the German equivalent to high school plus junior college, I majored in mathematics and physics and studied Latin for the language requirement, so I couldn't speak much English when I came to this country. In medical school in Germany, I thought biochemistry was the most interesting subject. I remember that one day the professor

show abstract

Assignments of the human genes for lactate dehydrogenase-A and thymidine kinase to specific chromosomal regions

Cited by 21 publications

References 1 publication

Activation of human beta-globin genes from nonerythroid cells by fusion with murine erythroleukemia cells.

Activation of human beta-globin genes from nonerythroid cells by fusion with murine erythroleukemia cells.

Gene dose effect: intraband mapping of the <i>LDH A </i>locus using cells from four individuals with different interstitial deletions of 11p

2012 William Allan Award: Adventures in Cytogenetics1

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