Assisted hatching close to inner cell mass (ICM) increases clinical outcomes of frozen-thawed blastocyst transfer cycles in unexplained or refractory repeated implantation failure patients

Ryu, Mi Jin; Kim, H.S.; Lee, J.H.; Kim, M.H.; Jeong, Hae Kyung; Chung, Myung-Jin

doi:10.1016/j.fertnstert.2014.07.1097

Cited by 2 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Later, Ren et al showed that the site of assisted hatching has no influence on the implantation, pregnancy, and live birth rate in human vitrified-warmed blastocysts [43]. In contrast to that, the results presented by Ryu et al a year later [44] confirmed that, in certain patients, AH close to the ICM of frozen-thawed blastocysts demonstrated significantly higher clinical outcomes (clinical pregnancy and implantation rates) than AH close to the TE. Rather, controversial data regarding the most preferential site of embryo hatching in humans and other mammalian species have been obtained so far.…”

Section: Discussionmentioning

confidence: 97%

See 1 more Smart Citation

Controlled hatching at the prescribed site using femtosecond laser for zona pellucida drilling at the early blastocyst stage

Ilina

Khramova

Ivanova

et al. 2020

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose To study whether the application of femtosecond laser pulses for zona pellucida (ZP) drilling of blastocysts at the embryonic or abembryonic poles can promote hatching to start immediately through the hole formed and ensure high hatching rates and embryo viability. Methods Mouse blastocyst (E3.5) ZP were microdissected with femtosecond laser pulses (514-nm wavelength, 280-fs pulse duration, 2.5-kHz repetition rate) close to the trophoblast or inner cell mass (ICM). The sizes of the holes formed were in the range of 4.5-8.5 μm. Additional longitudinal incisions (5-7-μm long) on either side of the hole were created to determine whether hatching had started at the correct position. Embryos post-laser-assisted ZP drilling and intact embryos were cultured under standard conditions for 2 days; embryo quality was assessed twice daily. The hatching rates and in vitro and in vivo implantation rates (only for embryos with ZP dissected close to the ICM) were estimated. Results Femtosecond laser-assisted ZP drilling at the early blastocyst stage facilitated embryo hatching to start at the artificial opening with probability approaching 100%. Despite the artificial opening's small size, no embryo trapping during hatching was observed. Both experimental groups had higher hatching rates than the control groups (93.3-94.7% vs. 83.3-85.7%, respectively). The in vitro implantation rate was comparable with that of the control group (92.3% vs. 95.4%). No statistically significant differences were obtained in the in vivo implantation rates between the experimental and control groups. Conclusions Blastocyst-stage femtosecond laser microsurgery of ZP is fast and delicate and enables the hatching process to be initiated in a controlled manner through a relatively small opening, with no embryo trapping.

show abstract

Section: Discussionmentioning

confidence: 97%

“…The results of ZP drilling at blastocyst stage in locations close to either the TE or ICM have recently been reported [42][43][44][52][53][54][55][56] and are summarized in Table 3. As a rule, an infrared diode laser (λ = 1.48 μm) is used to create a hole.…”

Section: Discussionmentioning

confidence: 99%

Controlled hatching at the prescribed site using femtosecond laser for zona pellucida drilling at the early blastocyst stage

Ilina

Khramova

Ivanova

et al. 2020

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…Laser-assisted hatching is usually performed on cleavage-stage embryos. Only a limited number of studies has reported performing LAH on blastocysts yet [50][51][52][53][54]. Results from the various studies are very controversial.…”

Section: Ultrashort Laser Microsurgery Of Preimplantation Embryos For Application In Artmentioning

confidence: 99%

From Zygote to Blastocyst: Application of Ultrashort Lasers in the Field of Assisted Reproduction and Developmental Biology

Ilina

Ситников

2021

Diagnostics

View full text Add to dashboard Cite

Although the use of lasers in medical diagnosis and therapies, as well as in fundamental biomedical research is now almost routine, advanced laser sources and new laser-based methods continue to emerge. Due to the unique ability of ultrashort laser pulses to deposit energy into a microscopic volume in the bulk of a transparent material without disrupting the surrounding tissues, the ultrashort laser-based microsurgery of cells and subcellular components within structurally complex and fragile specimens such as embryos is becoming an important tool in developmental biology and reproductive medicine. In this review, we discuss the mechanisms of ultrashort laser pulse interaction with the matter, advantages of their application for oocyte and preimplantation embryo microsurgery (e.g., for oocyte/blastomere enucleation and embryonic cell fusion), as well as for nonlinear optical microscopy for studying the dynamics of embryonic development and embryo quality assessment. Moreover, we focus on ultrashort laser-based approaches and techniques that are increasingly being applied in the fundamental research and have the potential for successful translation into the IVF (in vitro fertilization) clinics, such as laser-mediated individual embryo labelling and controlled laser-assisted hatching.

show abstract

Assisted hatching close to inner cell mass (ICM) increases clinical outcomes of frozen-thawed blastocyst transfer cycles in unexplained or refractory repeated implantation failure patients

Cited by 2 publications

References 0 publications

Controlled hatching at the prescribed site using femtosecond laser for zona pellucida drilling at the early blastocyst stage

Controlled hatching at the prescribed site using femtosecond laser for zona pellucida drilling at the early blastocyst stage

From Zygote to Blastocyst: Application of Ultrashort Lasers in the Field of Assisted Reproduction and Developmental Biology

Contact Info

Product

Resources

About