Assisted hatching on assisted conception (IVF and ICSI)

Sangeeta, Das; Blake, Debbie; Farquhar, Cindy; Seif, Mourad Mw

doi:10.1002/14651858.cd001894.pub4

Cited by 45 publications

(31 citation statements)

References 63 publications

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“…In the past decade, AH has been offered to older patients as well as to those with frozen-thawed embryos or who have suffered recurrent IVF-ET failure. The effect of AH, however, is still debatable [6][7][8][9]. Assisted hatching was first described by Cohen et al who reported the first pregnancy after assisted hatching in 1988 [10].…”

Section: Introductionmentioning

confidence: 99%

Assisted hatching in assisted reproduction: a state of the art

Hammadeh

Fischer-Hammadeh

Ali

2010

J Assist Reprod Genet

106

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The World Health Organization estimates that one in six couples experience some delay in conception and an increasing number require treatment by the assisted conception (AC) procedures of in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI).The implantation rate of embryos resulting from in vitro fertilization cycles is generally less than 20%. The exposure of oocytes and embryos to the artificial conditions of in vitro culture may have negative effects on the embryo's ability to undergo normal hatching, resulting in low rates of implantation following IVF and embryo transfer. Human embryos resulting from superovulation develop more slowly in vitro compared to embryos in vivo, manifest a relatively high degree of cytogenetic abnormalities and undergo cellular fragmentation. Artificially disrupting the zona pellucida is known as assisted hatching (AH) and there is some evidence that embryos that have undergone zona manipulation for assisted hatching tend to implant one day earlier than unhatched embryos. A variety of techniques have since been employed to assist embryo hatching, including partial mechanical zona dissection, zona drilling and zona thinning, making use of acid tyrodes, proteinases, piezon vibrator manipulators and lasers. This review will consider the impact of IVF conditions on zona pellucida physiology, zona hardening, different techniques of assisted hatching, who may benefit from assisted hatching and potential hazards.

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Section: Introductionmentioning

confidence: 99%

Assisted hatching in assisted reproduction: a state of the art

Hammadeh

Fischer-Hammadeh

Ali

2010

J Assist Reprod Genet

106

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“…Some studies have reported significant improvements in implantation rate and PR with the application of AH to treatment cycles by "poor prognosis" patients, defined by a history of repeated IVF-embryo transfer failures, elevated FSH, or advanced age (Toulis et al, 2010;Das et al, 2009). However, pooled analysis of such heterogeneous populations leaves ambiguity regarding whether all of these different reasons for a poor prognosis are responsive to AH.…”

Section: Disscusionmentioning

confidence: 99%

Clinical Pregnancy After Assisted Hatching Rate in Young women undergoing Intra Cytoplasmic Sperm Injection

Al-Sunaidi¹

2012

American Journal of Applied Sciences

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Problem statement:The ability of a blast cyst to hatch, or escape, from the Zona Pellucida (ZP) that surrounds and protects the embryo during its first few days of development is one of many critical events that must occur for successful reproduction. Implantation of the embryo in the uterine lining is impossible unless hatching occurs. Hard or thick ZP will therefore impaire the implantation process and reduce the pregnancy rate. Approach: Retrospective analysis of patients' records who had ICSI cycles comparing pregnancy rate for women with and without assisted hatching between Jan and Dec 2010, this was to evaluate the overall effect of AH on pregnancy rates in younger women (less than 37 years old) undergoing ICSI cycles at Mustasharak medical center, ABHA, Saudi Arabia. The studied population was divided into four different subgroups according to age and the differences in clinical pregnancy rate among the assisted hatching group and control group were calculated. Results: Clinical pregnancy rate was found to be significantly higher statistically in young women who underwent laser assisted hatching after ICSI. Conclusion: Laser assisted hatching has a positive impact on the clinical pregnancy rate following ICSI in young age group.

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“…The artificial rupture of the zona pellucida is known as assisted hatching (AH) and it has been proposed as a method for improving the implantation capacity of the embryos [4,5]. Although the clinical effect of AH in unselected patients is now still under debate, recent metaanalysis indicated AH was related to increased clinical pregnancy rate in women with frozen-thawed embryos [6,7].…”

Section: Introductionmentioning

confidence: 99%

Effect of the site of assisted hatching on vitrified-warmed blastocyst transfer cycles: a prospective randomized study

Ren

Liu

Chen

et al. 2013

J Assist Reprod Genet

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Purpose To assess the effect of assisted hatching (AH) site on the clinical outcomes in vitrified-warmed blastocyst transfer cycles. Methods A total of 160 women who underwent vitrifiedwarmed blastocyst transfer cycles were randomized to either the ICM group (AH performing at the site near the inner cell mess, ICM), or the TE group (AH performing at the site opposite to the ICM). AH with laser zona drilling was performed 20 or 30 min after thawing once the ICM can be detected. Clinical pregnancy rate, implantation rate, live birth rate and the occurrence rate of monozygotic twins (MZT) pregnancy after transfer of these two groups were compared. Results No significant difference was found in the clinical pregnancy rate (63.8 % vs. 67.5 %), implantation rate (51.7 % vs. 53.6 %) and live birth rate (57.5 % vs. 62.5 %) between the ICM group and the TE group. The occurrence rate of MZT was comparable between the two groups (3.9 % vs. 5.6 %). Conclusions The site of assisted hatching has no influence on the implantation, pregnancy and live birth rate in human vitrified-warmed blastocyst transfer cycles.

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Assisted hatching on assisted conception (IVF and ICSI)

Cited by 45 publications

References 63 publications

Assisted hatching in assisted reproduction: a state of the art

Assisted hatching in assisted reproduction: a state of the art

Clinical Pregnancy After Assisted Hatching Rate in Young women undergoing Intra Cytoplasmic Sperm Injection

Effect of the site of assisted hatching on vitrified-warmed blastocyst transfer cycles: a prospective randomized study

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