Association analysis of single nucleotide polymorphisms in DGAT1, TG and FABP4 genes and intramuscular fat in crossbred Bos taurus cattle

Pannier, L.; Mullen, Anne Maria; Hamill, Ruth M.; Stapleton, P.C.; Sweeney, T.

doi:10.1016/j.meatsci.2010.02.025

Cited by 71 publications

(55 citation statements)

References 19 publications

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“…However, in a case of this study, the T354C SNP of TG gene was not associated with marbling score in Hanwoo. Similar results were found in crossbred cattle in Ireland, the SNPs in DGAT1, TG and FABP4 had no association with intramuscular fat (IMF) level in crossbreed Bos taurus beef cattle (Pannier et al, 2010).…”

Section: Discussionsupporting

confidence: 68%

Identification of SNPs in TG and EDG1 genes and their relationships with carcass traits in Korean cattle (Hanwoo)

Cahyadi¹,

Maharani²,

Ryoo³

et al. 2012

CNU Journal of Agricultural Science

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: Thyroglobulin (TG) gene was known to be regulated fat cell growth and differentiation and the endothelial differentiation sphingolipid G-protein-coupled receptor 1 (EDG1) gene involves blood vessel formation and known to be affecting carcass traits in beef cattle. The aim of this study was to identify the single nucleotide polymorphisms (SNPs) in both TG and EDG1 genes and to analyze the association with carcass traits in Korean cattle (Hanwoo). The T354C SNP in TG gene located at the 3' flanking region and c.-312A>G SNP located at 3'-UTR of EDG1 gene were used for genotyping the animals using PCR-RFLP method. Three genotypes were identified in T354C SNP in TG gene and only two AA and AG genotypes were observed for the c.-312A>G SNP in EDG1 gene. The results indicated that T354C SNP in TG gene was not significantly associated with carcass traits. However, the c.-312A>G SNP in EDG1 gene had significant effects on backfat thickness (BF) and yield index (YI). These results may provide valuable information for further candidate gene studies affecting carcass traits in Korean cattle and may use as marker assisted selection for improving the quality of meat in Hanwoo.

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Section: Discussionsupporting

confidence: 68%

Identification of SNPs in TG and EDG1 genes and their relationships with carcass traits in Korean cattle (Hanwoo)

Cahyadi¹,

Maharani²,

Ryoo³

et al. 2012

CNU Journal of Agricultural Science

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“…From several biological perspectives cattle marbling remains intractable. It has not yielded to genome-wide association studies -that is, there are no reliable DNA markers that explain substantial amounts of phenotypic across populations and breeds (Pannier et al, 2010). However, application of the latest thinking in network theory has been exploited to reverse engineer a plausible set of contributing regulatory molecules (PPARGC1A, HNF4G, FOXP3) based on single nucleotide polymorphism association data across three breed types, Bos taurus, Bos indicus and tropical composites (Ramayo-Caldas et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Longitudinal muscle gene expression patterns associated with differential intramuscular fat in cattle

Hudson

Reverter

Greenwood

et al. 2015

Animal

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Intramuscular fat (IMF) can improve meat product quality through its impact on flavour and juiciness. High marbling cuts can command premium prices in some countries and grading systems, but there is substantial cost involved in choosing to grain feed animals in an effort to deposit more IMF. There would be value in developing methods to predict predisposition to 'marble' well. Unfortunately, the biological mechanisms underpinning marbling remain a mystery: the key adipocyte cell populations have not been defined, there are no reliable DNA markers, no known (if any) causal mutations and gene expression analyses in the main have tended to characterise increases in expression of end-point fat metabolism proteins such as the fatty acid-binding proteins. To shed light on expression-based markers of marbling potential, we contrasted LD gene expression in high IMF Wagyu cross animals with a low IMF Piedmontese cross at various time points. The expected divergence in the fat metabolism genes FABP4, THRSP, CIDEC and ACACA between the breeds occurs surprisingly late in postnatal development at about 20 months. On the other hand, divergent expression of WISP2, RAI14 and CYP4F2 was discovered in animals at or before 12 months of age, suggesting these genes may have potential as earlier predictors of marbling potential. In line with other researchers, we found intriguing links between IMF development and connective tissue remodelling. WISP2 -a novel adipokine highly expressed and secreted by adipose precursor cells and an inhibitor of the pro-fibrotic connective tissue growth factor -emerges as a particularly attractive candidate. It is relatively upregulated in high marbling Wagyu before admission to feedlotting, somewhere between 7 and 12 months. This difference is subsequently maintained until 25 months, but not thereafter. RAI14, thought to play a role in porcine adipocyte differentiation and with links to retinoic acid metabolism, has an unusual expression profile. Its expression level increases monotonically with postnatal development, and is always higher in Wagyu than Piedmontese. Strong, sustained upregulation of the anti-inflammatory CYP4F2 in Piedmontese is consistent with Wagyu adiposity being a pro-inflammatory state. Application of regulatory impact factor analysis, a network method for identifying causal effector molecules, suggests marbling roles for transcription factors previously implicated in (1) the formation of liposarcoma (unconstrained fatty masses) (YEATS4, MDM2), (2) adipogenesis (CREBL2, SP1, STAT1) and (3) inflammation (ISGF3G, HOXB13, PML).

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“…Casas et al (2005) reported that the marker was associated with fat thickness and LMA, but it was not associated with MS in Brahman cattle. No significant association was detected between the TG5 marker and IMF in Irish cattle and American Simmental (Rincker et al, 2006;Pannier et al, 2010). In Korean cattle, an association between TG5 and MS was detected, but the CC and CT genotypes were associated with a higher MS than the TT genotype (Shin and Chung, 2007).…”

Section: Discussionmentioning

confidence: 78%

“…Because of the important role of the 5ꞌ-flanking region in regulating gene transcription, the TG5 variation in the 5'-flanking region of the TG gene has been identified as having a significant association with the marbling score and is used in marker-assisted selection programs to improve the performance of intramuscular fat content in beef production (Barendse, 1999;Barendse et al, 2004;Burrell et al, 2004). However, other studies did not detect associations between the TG5 marker and fat deposition traits, or they obtained the opposite result (Casas et al, 2005;Rincker et al, 2006;Shin and Chung, 2007;Pannier et al, 2010).…”

Section: Introductionmentioning

confidence: 92%

Investigation of TG gene variants and their effects on growth, carcass composition, and meat quality traits in Chinese steers

Zhang¹,

Gan²,

Hou³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Thyroid hormones play an important role in regulating metabolism and can affect metabolism-related traits such as fat deposition. The thyroglobulin (TG) gene produces the precursor of thyroid hormones and has been proposed as a candidate gene for a quantitative trait locus with an effect on fat deposition. In this study, we identified 4 novel single nucleotide polymorphisms (SNPs) in the 5' flanking region of the TG gene using a DNA sequencing method. The SNP marker association analysis indicated that the T1355C SNPs were significantly associated with meat percentage (P < 0.05). A significant association between the G1356A polymorphism and live weight and loin muscle area was also detected (P < 0.05). However, no significant association was found between 4 SNPs and the other growth, carcass composition, and meat quality traits including intramuscular fat. The results of this study suggest SNPs in 5ꞌ-flanking region of the bovine TG gene that TG gene-specific SNPs may be a useful marker for growth traits in marker-assisted selection programs in beef cattle.

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Association analysis of single nucleotide polymorphisms in DGAT1, TG and FABP4 genes and intramuscular fat in crossbred Bos taurus cattle

Cited by 71 publications

References 19 publications

Identification of SNPs in TG and EDG1 genes and their relationships with carcass traits in Korean cattle (Hanwoo)

Identification of SNPs in TG and EDG1 genes and their relationships with carcass traits in Korean cattle (Hanwoo)

Longitudinal muscle gene expression patterns associated with differential intramuscular fat in cattle

Investigation of TG gene variants and their effects on growth, carcass composition, and meat quality traits in Chinese steers

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