Association between downexpression of MiR-203 and poor prognosis in non-small cell lung cancer patients

Tang, Rongjiang; Tang, Zhong; Dang, Yi‐Wu; Zhang, X.; Li, P.; Chen, G.

doi:10.1007/s12094-015-1377-9

Cited by 28 publications

(20 citation statements)

References 36 publications

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“…miR‐203 is another microRNA frequently deregulated in different types of cancer (Li, Gao, & Zhang, ; Tang et al., ; Tian et al., ; Zhao et al., ). Focusing on this specific miRNA, our findings are corroborating previous studies reporting a tumor suppressor role of miR‐203 in HNSCC, as miR‐203 expression was significantly downregulated in patients with lymph node metastasis (Benaich et al., ; Obayashi et al., ).…”

Section: Discussionmentioning

confidence: 99%

Clinicopathological significance of miR‐26, miR‐107, miR‐125b, and miR‐203 in head and neck carcinomas

et al. 2018

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Section: Discussionmentioning

confidence: 99%

Clinicopathological significance of miR‐26, miR‐107, miR‐125b, and miR‐203 in head and neck carcinomas

et al. 2018

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“…Before transfection, NSCLC cells were plated in 96-well plates at 2.5 × 10 3 cells per well and maintained at 37°C for 24 h. Blank control, negative mimic control, miR-30d-5p mimic, negative inhibitor control and miR30d-5p inhibitor (Ambion) were transfected in NSCLC cell lines at a final concentration of 60 nmol/L with Lipofectamine 2000 following the manufacturer's instructions. The concentration for transfections was determined based on previous studies [28,34]. MiR-30d-5p expression was detected with RT-qPCR in Applied Biosystems PCR7900 system as described previously [29,30,35,36].…”

Section: In Vitro Effect Of Mir-30d-5p On the Biological Process Of Nmentioning

confidence: 99%

Expression Signature and Role of miR-30d-5p in Non-Small Cell Lung Cancer: a Comprehensive Study Based on in Silico Analysis of Public Databases and in Vitro Experiments

et al. 2018

Cell Physiol Biochem

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Background/Aims: The purpose of this study was to probe the clinico-pathological significance and the underlying mechanism of miR-30d-5p expression in non-small cell lung cancer (NSCLC). Methods: We initially examined the level of miR-30d-5p expression in NSCLC and non-cancer tissues using RT-qPCR. Then, a series of validation analyses including a meta-analysis of data from microarray chips in Gene Expression Omnibus (GEO), data mining of the cancer genome atlas (TCGA) and an integrated meta-analysis incorporating GEO microarray chips, TCGA data, in-house RT-qPCR and literature studies were performed to examine the clinico-pathological value of miR-30d-5p expression in NSCLC. In vitro experiments were further conducted to investigate the impact of miR-30d-5p on NSCLC cell growth. The molecular mechanism by which miR-30d-5p regulates the pathogenesis of NSCLC was probed through a bioinformatics analysis of its target genes. Moreover, dual luciferase reporter assay was conducted to verify the targeting regulatory relationship between miR-30d-5p and CCNE2. Results: Based on results from RT-qPCR, GEO meta-analysis, TCGA data mining and the integrated meta-analysis incorporating GEO microarray chips, TCGA data, in-house RT-qPCR and literature studies, miR-30d-5p expression was decreased in NSCLC tissues, and patients with NSCLC who presented with lower miR-30d-5p expression tended to display an advanced clinical progression. Significant pathways including the Mucin type O-glycan biosynthesis pathway, cell cycle pathway and cysteine and methionine metabolism pathway (all P< 0.05) revealed potential roles of the target genes of miR-30d-5p in the oncogenesis of NSCLC. Results from in vitro experiments indicated that miR-30d-5p could attenuate proliferation and viability of NSCLC cells. Among the 12 identified hub genes, nine genes including E2F3, CCNE2, SKP2, CDK6, TFDP1, LDHA, GOT2, DNMT3B and ST6GALNAC1 were validated by Pearson’s correlation test and the human protein atlas (HPA) database as targets of miR-30d-5p with higher probability. Specifically, dual luciferase reporter assay confirmed that CCNE2 was directly targeted by miR-30d-5p. Conclusion: In summary, miR-30d-5p expression is decreased in NSCLC, and it might play the role as tumor suppressor in NSCLC by regulating target genes.

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“…Previous studies provided evidence of a decreased expression of miR-203 in a variety of lung cancer tissue samples in contrast to the expression of normal human bronchial epithelial cells or normal lung tissues, respectively (43,44). Also, reduced expression of miR-203 was found to be associated with metastatic tumors (45).…”

Section: Discussionmentioning

confidence: 99%

H19 contributes to poor clinical features in NSCLC patients and leads to enhanced invasion in A549 cells through regulating miRNA‑203‑mediated epithelial‑mesenchymal transition

Zheng

Feng

et al. 2018

Oncol Lett

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Recent studies have demonstrated that the overexpression of H19 may contribute towards development of tumorigenesis in various types of cancer. To investigate the role of H19 in the development of non-small cell lung cancer (NSCLC), 76 NSCLC tissues samples and their adjacent normal tissue samples were collected. Expression level of H19, and its association with clinicopathological features and overall survival was analyzed. It was found that compared with normal adjacent tissues, H19 expression was elevated in NSCLC tissues along with a decreased miR-203 expression level. It was also found that patients who were in advanced clinical stages had a higher H19 and a lower miR-203 expression compared to normal tissues. The overall survival time of patients with higher H19 expression was shorter compared with the lower H19 expression group. Upregulation of A549 enhanced cell proliferation and promoted invasion. Overexpression of H19 stimulated the epithelial-mesenchymal transition (EMT) process in lung cancer cells and demonstrated typical morphological characteristics of EMT. The level of mesenchymal marker protein, such as Vimentin and SNAI1 increased; while CDH1 protein level decreased. Also, H19 negatively regulated miR-203. Inhibition of H19 attenuated miR-203 induced EMT process. Upregulation of H19 contributes to poor clinical features in patients with NSCLC, induces occurrence of EMT, promotes proliferation and stimulates cell invasion in NSCLC cell line through regulating miRNA-203 mediated EMT.

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Association between downexpression of MiR-203 and poor prognosis in non-small cell lung cancer patients

Abstract: MiR-203 could become a prognostic predictor in NSCLC and may be a new target for the molecular therapy of NSCLC patients.

Cited by 28 publications

References 36 publications

Clinicopathological significance of miR‐26, miR‐107, miR‐125b, and miR‐203 in head and neck carcinomas

Clinicopathological significance of miR‐26, miR‐107, miR‐125b, and miR‐203 in head and neck carcinomas

Expression Signature and Role of miR-30d-5p in Non-Small Cell Lung Cancer: a Comprehensive Study Based on in Silico Analysis of Public Databases and in Vitro Experiments

H19 contributes to poor clinical features in NSCLC patients and leads to enhanced invasion in A549 cells through regulating miRNA‑203‑mediated epithelial‑mesenchymal transition

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