Association between gut microbiota and diapause preparation in the cabbage beetle: a new perspective for studying insect diapause

Liu, Wen; Yi, Li; Guo, Shuang; Yin, Huiming; Lei, Chaoliang; Wang, Xiao‐Ping

doi:10.1038/srep38900

Cited by 41 publications

(20 citation statements)

References 42 publications

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“…Previous studies detected Xanthomonadaceae in soil and plant samples 75,76 . Weeksellaceae has been found to be a dominant element of the microbiome in B. carambolae larvae and pupae 36 and Colaphellus bowringi (Coleoptera: Chrysomelidae) (Cabbage beetle) 28 .…”

Section: Discussionmentioning

confidence: 99%

Next-Generation Sequencing reveals relationship between the larval microbiome and food substrate in the polyphagous Queensland fruit fly

Majumder

Sutcliffe

Taylor

et al. 2019

Sci Rep

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Insects typically host substantial microbial communities (the ‘microbiome’) that can serve as a vital source of nutrients and also acts as a modulator of immune function. While recent studies have shown that diet is an important influence on the gut microbiome, very little is known about the dynamics underpinning microbial acquisition from natural food sources. Here, we addressed this gap by comparing the microbiome of larvae of the polyphagous fruit fly Bactrocera tryoni (‘Queensland fruit fly’) that were collected from five different fruit types (sapodilla [from two different localities], hog plum, pomegranate, green apple, and quince) from North-east to South-east Australia. Using Next-Generation Sequencing on the Illumina MiSeq platform, we addressed two questions: (1) what bacterial communities are available to B. tryoni larvae from different host fruit; and (2) how does the microbiome vary between B. tryoni larvae and its host fruit? The abundant bacterial taxa were similar for B. tryoni larvae from different fruit despite significant differences in the overall microbial community compositions. Our study suggests that the bacterial community structure of B. tryoni larvae is related less to the host fruit (diet) microbiome and more to vertical transfer of the microbiome during egg laying. Our findings also suggest that geographic location may play a quite limited role in structuring of larval microbiomes. This is the first study to use Next-Generation Sequencing to analyze the microbiome of B. tryoni larvae together with the host fruit, an approach that has enabled greatly increased resolution of relationships between the insect’s microbiome and that of the surrounding host tissues.

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Section: Discussionmentioning

confidence: 99%

Next-Generation Sequencing reveals relationship between the larval microbiome and food substrate in the polyphagous Queensland fruit fly

Majumder

Sutcliffe

Taylor

et al. 2019

Sci Rep

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“…Many species cease all feeding at the onset of initiation (including one fish; Penttinen & Holopainen, 1992), and animals often dramatically decrease their digestive system volume (Cramp et al., 2009; Hahn & Denlinger, 2011; Hume et al., 2002; Martin et al., 2008; Naya et al., 2009). The gut microbiome of many animals is also modified as animals begin fasting during initiation (Bailey et al., 2010; Carey et al., 2013; Gossling et al., 1982; Liu et al., 2016; Secor & Carey, 2016; Sommer et al., 2016). For animals that consume food intermittently during dormancy, such as food‐caching rodents, some bats, bears and some insect larvae (Avery, 1985; Boyles et al., 2006; Goddeeris et al., 2001; Humphries et al., 2003; Kirby et al., 2019; Krofel et al., 2016; López et al., 1995; Pigeon et al., 2016; Turbill, 2008), digestive system volume may still be reduced, but maintaining a more active gut throughout winter is likely to increase overall maintenance costs (Humphries et al., 2003).…”

Section: Integrating Animal Dormancy Research In a Universal Frameworkmentioning

confidence: 99%

A unifying, eco‐physiological framework for animal dormancy

2020

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1. Various animals across the tree of life express some form of programmed dormancy (e.g. hibernation, diapause) to maximize fitness in highly seasonal environments. The integrated phenotype of animals undergoing programmed dormancy is strikingly similar among diverse groups; however, research on programmed dormancy has historically been phylogenetically siloed. A broad comparative approach could clarify new angles for answering fundamental questions about programmed dormancy evolution. 2. To advance this approach, we present a cross-taxonomic framework describing dimensions that distinguish animal dormancies and provide a set of core traits that animals regulate as they progress through the eco-physiological phases of deep, programmed dormancy. 3. We use this universal framework to explore the ultimate drivers and evolutionary consequences of dormancy across the tree of life. Deep, programmed dormancy appears to be a predictable and repeated adaptation to highly seasonal environments that draws on a conserved suite of ancestral traits. We highlight evidence for molecular convergence in signalling pathways coordinating environmental sensing and energy metabolism in the insect and mammal lineages, separated by 700 million years of evolution and representing independent colonizations of highly seasonal environments. 4. Lastly, we discuss the utility of this new framework and highlight opportunities and challenges for researchers to continue advancing our understanding of dormancy through a broad, comparative lens.

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“…has been reported as being responsible for the increase of glucose intolerance in obese humans and mice [58] and Delftia spp. has been commonly found in the gastrointestinal microbiota of insects and fishes [59,60].…”

Section: Level Of Information Attained With Culturomics Vs Microbialmentioning

confidence: 99%

Crosstalk Between Culturomics and Microbial Profiling of Egyptian Mongoose (Herpestes ichneumon) Gut Microbiome

et al. 2020

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Recently, we unveiled taxonomical and functional differences in Egyptian mongoose (Herpestes ichneumon) gut microbiota across sex and age classes by microbial profiling. In this study, we generate, through culturomics, extended baseline information on the culturable bacterial and fungal microbiome of the species using the same specimens as models. Firstly, this strategy enabled us to explore cultivable microbial community differences across sexes and to ascertain the influence exerted by biological and environmental contexts of each host in its microbiota signature. Secondly, it permitted us to compare the culturomics and microbial profiling approaches and their ability to provide information on mongoose gut microbiota. In agreement with microbial profiling, culturomics showed that the core gut cultivable microbiota of the mongoose is dominated by Firmicutes and, as previously found, is able to distinguish sex- and age class-specific genera. Additional information could be obtained by culturomics, with six new genera unveiled. Richness indices and the Shannon index were concordant between culture-dependent and culture-independent approaches, highlighting significantly higher values when using microbial profiling. However, the Simpson index underlined higher values for the culturomics-generated data. These contrasting results were due to a differential influence of dominant and rare taxa on those indices. Beta diversity analyses of culturable microbiota showed similarities between adults and juveniles, but not in the data series originated from microbial profiling. Additionally, whereas the microbial profiling indicated that there were several bioenvironmental features related to the bacterial gut microbiota of the Egyptian mongoose, a clear association between microbiota and bioenvironmental features could not be established through culturomics. The discrepancies found between the data generated by the two methodologies and the underlying inferences, both in terms of β-diversity and role of bioenvironmental features, confirm that culture-independent, sequence-based methods have a higher ability to assess, at a fine scale, the influence of abiotic and biotic factors on the microbial community composition of mongoose’ gut. However, when used in a complementary perspective, this knowledge can be expanded by culturomics.

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Association between gut microbiota and diapause preparation in the cabbage beetle: a new perspective for studying insect diapause

Cited by 41 publications

References 42 publications

Next-Generation Sequencing reveals relationship between the larval microbiome and food substrate in the polyphagous Queensland fruit fly

Next-Generation Sequencing reveals relationship between the larval microbiome and food substrate in the polyphagous Queensland fruit fly

A unifying, eco‐physiological framework for animal dormancy

Crosstalk Between Culturomics and Microbial Profiling of Egyptian Mongoose (Herpestes ichneumon) Gut Microbiome

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