Association between Immunohistochemistry Markers and Tumor Features and Their Diagnostic and Prognostic Values in Intrahepatic Cholangiocarcinoma

Abstract

This study investigated the expression of some frequently used immunohistochemistry (IHC) markers. Besides, we evaluated their correlations with the clinical features and outcomes of intrahepatic cholangiocarcinoma (ICC). Patients who underwent surgical removal of the ICC tumors were followed up for 4 years. The paraffin-embedded sections were used to obtain different markers, including CK7, CK19, CK20, CDX2, Glypican3, Hepa1, Ki-67, Villin, and SATB1. Overall survival in relation to IHC marker expression patterns and other clinical characteristics was evaluated by Kaplan-Meier survival curve and log-rank test, followed by the Cox proportional hazard model (to evaluate the relationship between multiple factors and the overall postoperative survival). A total of 122 ICC patients (67 males and 55 females, averagely aged 57.75) were included in this study. There were 44 cases with vascular invasion, 46 cases with lymphatic metastasis, and 13 cases with distant metastasis. CK7 was negatively correlated with lymphatic metastasis; and in distant-metastasis cases, the positive ratio of SATB1 was lower. Interestingly, SATB1 expression indicated a poorer survival, while Villin expression was associated with a better survival. The COX regression analysis showed that female was a protective factor versus male, Villin expression was a strong protective factor, and Ki-67 expression was correlated with a poor survival. Together, IHC markers are associated with tumor features and postoperative survival, especially for SATB1 as a risk factor and Villin as a protective marker, and female ICC patients may have better survival than males.

Expression of Ki-67 in premalignant and malignant lesion of cervix in tertiary care hospital in Uttar Pradesh

“…26 In other studies, the mean age of patients has been reported to range from 37.40 years to 47 years. 13,27,28 A much younger age profile of the patients was reported by an author in 2019 who reported the median age of premalignant and malignant group patients in 25 to 28 years range. 29 In the present study, majority of patients (55%) were in age group 40-49 years.…”

Predictors of Distant Metastasis and Prognosis in Newly Diagnosed T1 Intrahepatic Cholangiocarcinoma

“…OR of lymph node metastasis was up to 2.81, indicating the close association between lymph node metastasis and DM, which is not surprising that lymph node could be a way to occur DM [ 18 ]. In previous studies [ 19 , 20 ], age was rarely used as a related variable for DM. However, this study found that the DM risk of patients aged 50 to 64 years was higher than other patients.…”

The Role of Immunohistochemistry in the Differential Diagnosis between Intrahepatic Cholangiocarcinoma, Hepatocellular Carcinoma and Liver Metastasis, as Well as Its Prognostic Value

“…A recent study conducted by He et al demonstrated a significant association between the post-surgery survival of iCCA patients and two immunohistochemical markers: while SATB1 indicated poor survival (median survival of 122 days vs. 347 days in SATB1-negative cases, p = 0.04), Villin-positive cases were associated with better OS, with direct correlation with Villin intensity (p = 0.002). This study recommended CK7 assessment in iCCA cases, since it was negatively correlated with lymphatic metastasis in their case series [41]. An interesting study conducted by Yeh et al in Taipei validated C-reactive protein (CRP) as a highly performant diagnostic marker for iCCA, with a 93.3% sensitivity and an 88.2% specificity.…”