Association between of polymorphism rs1801133 in MTHFR gene and diabetes mellitus type II for Iraqi patients

Abstract

Diabetes mellitus (DM) is a clinical syndrome caused by metabolic problems. Recently, researchers have recently concentrated their efforts on identifying additional risk variables, like as homocysteine (Hcy), a non-independent vascular risk factor linked to insulin resistance in type 2 diabetes mellitus (T2DM). Aimed our current study to association between of polymorphism rs1801133 in MTHFR gene and diabetes mellitus type II for Iraqi patients. Samples of blood were collected from Iraqi with T2DM (50 patenits) from auditors to specialized laboratories and consulting clinics in Baquba District / Diyala Governorate, Iraq, as well as 21 healthy participants, and their ages ranged (35-54 years). Genotyping was performed by PCR/SNP (specific primers), risk score for T2DM was determined by Hardy-Weinberg equilibrium (HWE). (HWE) was analyzed in T2DM patients and healthy participants, and it was discovered that the MTHFR (rs1801133) genotypes were in agreement with the equilibrium, with no clear differences (p>1.020-0.298) between the observed and expected genotype frequencies. When rs1801133 genotype and allele frequencies were compared in T2DM patients and healthy participants, it was shown that there were no significant differences in these frequencies.