Association between Single Nucleotide Polymorphisms in SIRT1 and SIRT2 Loci and Growth in Tibetan Sheep

Abstract

Silent information regulator 1 and 2 (SIRT1, 2) were NAD+-dependent histone or non-histone deacetylase, which emerged as key metabolic sensors in several tissues of mammals. In the present study, the search for polymorphisms within the ovine SIRT1 and SIRT2 loci as well as association analyses between SNPs and growth-related traits were performed in Tibetan sheep. To determine the expression pattern of SIRT1 and SIRT2 genes in Tibetan sheep, the quantitative real-time polymerase chain reaction (qPCR) analysis revealed that those two genes were widely expressed in diverse tissues. Expression of SIRT1 was less in abomasum of lamb, whereas it was greater in duodenum within adult stage. In the case of SIRT2, the greatest expression was observed in reticulum (lamb) and in muscle (adult), whereas the least expression was in liver for lamb and in kidney for adult animals. The association analysis demonstrated that g.3148 C > T polymorphism of SIRT1 affected heart girth (p = 0.002). The g.8074 T > A SNP of SIRT2 had a significant correlation with body weight (p = 0.011) and body length (p = 0.008). These findings suggested that the SIRT1 and SIRT2 polymorphism was involved in growth-related traits in Tibetan sheep, which may be considered to be genetic markers for improving the growth traits of Tibetan sheep.

Characterization of the circRNA–miRNA–mRNA Network to Reveal the Potential Functional ceRNAs Associated With Dynamic Changes in the Meat Quality of the Longissimus Thoracis Muscle in Tibetan Sheep at Different Growth Stages

“…Present studies are mainly focused on improving the meat quality and growth performance of Tibetan sheep. Previous studies have identified the gene FGFR1 to be involved in the growth-related traits, and henceforth, it was used as the genetic marker for improving the growth traits of the Tibetan sheep (3) The polymorphisms in SIRT1 and SIRT2 regulate the growth-related traits in Tibetan sheep (4). The quantitative genetic analysis revealed IRS1 to be associated with the growth-efficiency traits possibly useful for markerassisted selection in Chinese indigenous sheep (5).…”

Effects of nonsynonymous single nucleotide polymorphisms of the KIAA1217, SNTA1 and LTBP1 genes on the growth traits of Ujumqin sheep

“…In recent years, following the development of biotechnology and genome research, an increasing number of SNPs related to the growth traits of livestock and poultry have been discovered. Studies have shown that the g.3148C > T polymorphism of the SIRT1 gene affects the heart circumference of Tibetan sheep, and the g.8074 T > A polymorphism of the SIRT2 gene is significantly correlated with body weight and body length ( 40 ). Cao et al discovered OARX_76354330.1 and s64890.1 to be functional SNPs for growth traits of Hu sheep through genome-wide association analysis of body weight and identified three candidate genes related to body weight in Hu sheep ( 41 ): CAPN6 , ITGA11 and SCMH1 .…”

Whole-Genome Selective Scans Detect Genes Associated With Important Phenotypic Traits in Sheep (Ovis aries)

“…The recent availability of genome-wide SNPs gave a new momentum to identify the genetic variants underlying phenotypic traits ( Kijas et al, 2012 ; Xu and Li 2017 ; Gui et al, 2020 ; Abousoliman et al, 2021 ; Zhou et al, 2021 ). Previous studies have identified a number of candidate genes or variants associated with meat, growth, milk, wool, reproduction, horns and tails in sheep, most of which have employed the low-density SNPs (50K) (see the review in Xu and Li, 2017 ) or whole-genome sequences ( Li et al, 2020 ).…”