2005
DOI: 10.1002/art.21300
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Association between the abnormal expression of matrix‐degrading enzymes by human osteoarthritic chondrocytes and demethylation of specific CpG sites in the promoter regions

Abstract: Objective. To investigate whether the abnormal expression of matrix metalloproteinases (MMPs) 3, 9, and 13 and ADAMTS-4 by human osteoarthritic (OA) chondrocytes is associated with epigenetic "unsilencing."Methods. Cartilage was obtained from the femoral heads of 16 patients with OA and 10 control patients with femoral neck fracture. Chondrocytes with abnormal enzyme expression were immunolocalized. DNA was extracted, and the methylation status of the promoter regions of MMPs 3, 9, and 13 and ADAMTS-4 was anal… Show more

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Cited by 319 publications
(298 citation statements)
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“…54 Also, a variety of cytokines and chemokines stimulate the expression of MMPs. 55,56 The chemokine CXCL12 was reported to induce the expression of MMP-3 in chondrocytes and of MMP-9 in osteoclasts. 16,22 Furthermore, increased gelatinase and collagenase activity was described after CXCL12 stimulation in SFs.…”
Section: Dna Methylation Regulates the Expression Of Cxcl12 E Karouzamentioning
confidence: 99%
“…54 Also, a variety of cytokines and chemokines stimulate the expression of MMPs. 55,56 The chemokine CXCL12 was reported to induce the expression of MMP-3 in chondrocytes and of MMP-9 in osteoclasts. 16,22 Furthermore, increased gelatinase and collagenase activity was described after CXCL12 stimulation in SFs.…”
Section: Dna Methylation Regulates the Expression Of Cxcl12 E Karouzamentioning
confidence: 99%
“…However, only a few in vitro studies have indicated some possible involvement of DNA methylation in the maturation of cultured primary chondrocytes (12) and growth plates (13) or in the progression of degenerative joint diseases (14,15). Thus, it has not been determined whether epigenetic control is indeed involved in the in vivo process of chondrogenesis during normal development, and in particular, no data from human studies have been reported.…”
mentioning
confidence: 99%
“…14 A major alternative method is the Methylation Sensitive Restriction Enzyme (MSRE) method. 8,15,16 This utilizes the methylation sensitivity of the 53 currently commercially available restriction enzymes: When the CpG site is not methylated, the enzymes cleave the DNA and subsequent PCR amplification is abrogated. However, if the CpG site is methylated, the enzymes cannot cut and the DNA strand is amplified by PCR.…”
Section: Methods For Detection Of Dna Methylationmentioning
confidence: 99%
“…In some promoters, particular enzyme/primer combinations can uniquely identify the methylation status of specific CpG sites, as is the case for the MMP-13 promoter. 8 However, if the same enzyme cuts at several CpG sites that are located close together, as is the case for three AciI-cleavable sites in the MMP-9 promoter, 8 then all three sites will be assessed together. Moreover, not all CpG sites are cut by MSREs.…”
Section: Methods For Detection Of Dna Methylationmentioning
confidence: 99%
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