Association between urine and serum estradiol levels in in vitro fertilization cycles

Abstract

To study the correlation between urine and serum estradiol (E2) controlled ovarian hyperstimulation (COH). This is a cross-sectional analytical study that was conducted in a tertiary care hospital. Seventy-seven urine and blood samplings were collected from infertile women who were treated with COH. An electrochemiluminescent immunoassay was performed to evaluate E2 levels between urine and serum samples on the 6th day and the day of ovarian trigger. In addition, the correlations were evaluated between urine E2 level and number of follicles, retrieved, metaphase II oocytes, and fertilization rate. A sub-analysis was performed for age, responding status and BMI. Seventy-seven infertile women were recruited. The medians of serum and urine E2 level levels on the day 6th of ovarian stimulation were 833.20 pg/ml (IQR; 516.90–1371.00) and 3.67 (IQR; 2.84–4.81), respectively. On the day of ovarian trigger, the median of serum E2 level was 2113.00 pg/ml (IQR; 1382.00–3885.00) and urine E2 level (E2/creatinine) was 6.84 (IQR; 5.34–8.70). The correlation between serum and urine E2 level on day 6th was 0.53 and the day of ovarian trigger was 0.59, p < 0.001. Moreover, the correlations of urine E2 level on the day of ovarian trigger to number of follicles, number of oocytes retrieved, metaphase II oocytes and fertilization rate were 0.57, 0.58, 0.61, and 0.64 (p < 0.001). The urine E2 level was moderately correlated to serum E2, number of follicles growth, oocytes retrieved and fertilization rate.

At-home urine estrone-3-glucuronide quantification predicts oocyte retrieval outcomes comparably with serum estradiol

“…The study of urinary estrogen metabolites during gonadotropin stimulation has a long historical precedent ( 14 , 15 , 16 , 17 ). More recently, Chotboon et al ( 18 ) compared serum and urine concentrations on the same automated electrochemiluminescent immunoassay with antibodies directed against 17β E2 during stimulation for IVF and reported a correlation coefficient of 0.59, less than the level of correlation found in our current data ( r = 0.761). Similar to our primary analysis, they also calculated correlation coefficients of urine and serum E2 concentrations on trigger day with the number of total and MII oocytes, which were similar to those found in our study when confidence intervals are considered.…”

Ultrahigh-Sensitivity Detection of 17β-Estradiol

“…This may cause early skeletal maturation, resulting in the loss of potential stature, obesity, an increasing risk of breast cancer, and even social/emotional problems such as depression. Recently, the human-urine-based detection of E2 for its early diagnosis is gaining more popularity due to its patient-friendly and comfortable assay in comparison with serum-based assays [17][18][19][20][21], despite the fact that the E2 concentration present in human urine is much smaller than that in human serum [22]. As solutions to the aforementioned challenges associated with the limited sensitivity available from existing sensor strategies, attempts have been made with various detection mechanisms, such as plasmonic-nanoparticle-based sensors [23][24][25][26][27], fluorescence sensors [28][29][30][31][32], Raman-based sensors [33][34][35][36][37][38], electrochemical sensors [39][40][41][42][43][44][45][46][47][48][49], enzyme immunosensors [50,51], and others [52][53][54][55][56][57][58]…”