Association between xanthine dehydrogenase tag single nucleotide polymorphisms and essential hypertension

Abstract

The present study aimed to investigate the association between xanthine dehydrogenase (XDH) gene polymorphism and essential hypertension in the rural Han Chinese population of Fuxin, Liaoning. Han Chinese individuals, who had lived in rural areas of Fuxin, were selected as subjects for the present study. A total of 521 unrelated patients with hypertension were selected, along with a further 533 unrelated individuals with normal blood pressure, in order to serve as controls. Five tag single nucleotide polymorphisms (SNP) of the XDH gene were selected. An estimation of SNP allele frequency was determined using DNA pooling and pyrosequencing methods. Prior to Bonferroni correction, T allele frequency for rs206811 was significantly higher in patients with hypertension, as compared with the controls (64.1 vs. 59.4%; P=0.031); C allele frequency for rs1042039 was significantly higher in patients with hypertension, as compared with the controls (66.1 vs. 60.6%; P=0.011), C allele frequency for rs1054889 was significantly lower in patients with hypertension, as compared with the controls (38.8 vs. 44.8%; P=0.007); and A allele frequency for rs2073316 was significantly lower in patients with hypertension, as compared with the controls (29.2 vs. 34.4%; P=0.013). However, once a Bonferroni correction for multiple testing was applied, the XDH gene polymorphisms rs1042039, rs1054889, and rs2073316 were shown to be associated with hypertension (P=0.044, 0.035, and 0.039, respectively). These results suggest that the XDH gene polymorphisms rs1042039, rs1054889, and rs2073316 may be associated with hypertension in the rural Han Chinese population.

Identification of oxidative stress–related Xdh gene as a di(2‐ethylhexyl)phthalate (DEHP) target and the use of melatonin to alleviate the DEHP‐induced impairments in newborn mouse ovaries

“…Based on the results reported above, among these genes, we focused our attention on Xdh encoding a xanthine dehydrogenase which is a member of a group of hydroxylases involved in the oxidative metabolism. Namely, this protein is converted to xanthine oxidase, which produces ROS . The FPKM values of Xdh transcripts in the control and DEHP groups are shown in Figure D, and the FPKM values of Xdh in the DEHP groups were significantly upregulated compared with those of the control group.…”

“…Previous studies demonstrated that XDH and xanthine oxidase (XO) are single gene products, which exist in a single, interconvertible form . XDH was transformed into XO by reversible sulfhydryl oxidation or irreversible proteolytic modification, and both of them are related to the production of ROS . It was reported that the activities of XDH and XO in irradiated rats were significantly increased, while hepatic glutathione content, superoxide dismutase, and catalase were significantly decreased .…”

“…47 XDH was transformed into XO by reversible sulfhydryl oxidation or irreversible proteolytic modification, and both of them are related to the production of ROS. 36,37 It was reported that the activities of XDH and XO in irradiated rats were significantly increased, while hepatic glutathione content, superoxide dismutase, and catalase were significantly decreased. 38 Therefore, XDH or XO can act as a source of ROS, and production of ROS and associated DNA damage can lead to cellular apoptosis.…”

“…Namely, this protein is converted to xanthine oxidase, which produces ROS. 36,37 The FPKM values of Xdh transcripts in the control and DEHP groups are shown in Figure 4D, and the FPKM values of Xdh in the DEHP groups were significantly upregulated compared with those of the control group. RT-qPCR and Western blot for Xdh confirmed the DEHP induction of this enzyme found in the transcriptome analysis ( Figure 4E,F; P < 0.05).…”

C1QBP regulates apoptosis of renal cell carcinoma via modulating xanthine dehydrogenase (XDH) mediated ROS generation

“…XDH involves in the oxidative purine metabolism by catalyzing hypoxanthine to produce xanthine and ROS [26,27], and the activation of XDH generates oxidative stress [28]. We examined uorescent probe DCF (2',7'-dichloro uorescein, DCF) uorescence intensity represented as ROS level in RCC cells, the results showed that DCF uorescence was signi cantly decreased in C1QBP knockdown ACHN and 786-O cell lines (Fig.…”