Association of A‐FABP gene polymorphism and mRNA expression with intramuscular fat content (IMF) in Baicheng‐You chicken

Abstract

This study aims to assess the association of polymorphisms and mRNA expression of adipocyte‐type fatty acid‐binding protein (A‐FABP) with intramuscular fat (IMF) in the breast muscle (BM) and leg muscle (LM) of Baicheng‐You chickens (BYCs). A total of 180 chickens, including sixty black Baicheng‐You chickens (BBYCs), sixty silky Baicheng‐You chickens (SBYCs) and sixty white Baicheng‐You chickens (WBYCs), were reared from 1 to 120 day. A polymerase chain reaction–single‐strand conformation polymorphism strategy (PCR‐SSCP) was used to detect the polymorphism of the A‐FABP gene in the first exon, and the C51T silent mutational site was found. The IMF content with the AA genotype was significantly higher than that with the AG genotype (p = 0.0473) in the LM of WBYC. Thus, this site could be taken as a molecular marker in selecting a higher IMF content of LM in WBYC. A‐FABP gene mRNA expression in the BM and LM of BYCs was detected, and a significant positive correlation was observed in the LM of WBYC. These findings provide fundamental data that might be useful in further study of the role of the A‐FABP gene in IMF content and fatty metabolism in chickens.

Transcriptional insights into key genes and pathways controlling muscle lipid metabolism in broiler chickens

“…The upregulation of all these genes indicated a higher lipid biosynthesis in the HTG group. Previous studies have identified numerous candidate genes ( PPARs , FABPs , LPL , SCD , KLFs and ACSLs ) related to IMF deposition in chickens [7–10]. Most of these genes are associated with TG deposition in this study, indicating they might play an important role in IMF deposition by regulating TG metabolism.…”

The effects of plant extracts on lipid metabolism of chickens — A review

“…Three genotypes of the A-FABP gene first exon, AA, AB, and BB were examined by PCR-SSCP. The intramuscular fat content with the AA genotype was significantly higher than that with the AB genotype in the Baicheng-You Chickens [50], three-Yellow Chickens and Hetian-Black Chickens [51].…”

“…Three genotypes of the A-FABP gene first exon, AA, AB, and BB were examined by polymerase chain reaction-single-strand conformation polymorphism. The intramuscular fat content with the AA genotype was significantly higher than that with the AB genotype in the Baicheng-You Chickens [ 50 ], three-Yellow Chickens and Hetian-Black Chickens [ 51 ]. iii) According to the research of Fu et al [ 52 ], five transcriptional regulatory factors including CCAAT/enhancer binding protein α and β (CEBPα, β), PPARα, PPARγ, sterol-regulatory element binding proteins (SREBP-1) influenced the fat deposition of Beijing-You chickens.…”