Association of a synonymous mutation of the PGAM2 gene and growth traits in rabbits

Abstract

Phosphoglycerate mutase (PGAM2) catalyzes the conversion of 3-phosphoglycerate into 2-phosphoglycerate and releases energy during glycolysis in muscle tissues. PGAM2 has been considered as a candidate gene to influence growth, development, and carcass traits in livestock. The aim of this study was to investigate the association between polymorphisms of PGAM2 and growth traits in rabbits. Three single nucleotide polymorphisms (SNPs) were identified by direct sequencing in 20 random individuals from three breeds, including c.-10C>T, c.195C>T, and c.414+17C>T. The c.195C>T was genotyped by PCR-RFLP in a total of 222 rabbits of three breeds (Tianfu black, 53 animals; Ira, 91 animals; Champagne, 78 animals). The average allele frequency among the breeds was 0.52 for allele T and 0.48 for C. The heterozygosity and effective number of alleles were 0.4992 and 1.996, respectively. The association results revealed the CT genotype of c.195C>T was associated significantly (P < 0.05) with greater body weight at 84 days of age (BW84) and with average daily weight gain (ADG). However, association of the genotypes with other production traits was not observed. The results of this study suggested PGAM2 is one of the candidate genes affecting BW84 and ADG in the rabbit.

Associations between single nucleotide polymorphisms in multiple candidate genes and body weight in rabbits

“…Wu et al . [ 11 ] observed that no association was found between PGAM and BW at market. Sternstein et al .…”

Crossbreeding Effects and Polymorphic Associations of Genotypes of Gh Gene With Growth Traits in Rabbits

“…In China, Zhang et al (2012) found significant associations (P<0.05) between growth hormone receptor gene (GHR) and body weights in Tianfu Black, Ira and Champagne rabbits. Additionally, Wu et al (2015) reported significant association (P<0.05) between phosphorglycerate mutase gene (PGAM2) and growth traits in the same Chinese rabbit breeds. The differences between genotypes of GH gene in ½A½M cross rabbits were significant (P<0.05) in all body weights except body weight at 4 weeks and the CC genotype was absent ( Table 10).…”

“…There were significant associations between the genotypes SNP and body weights. In this concept, the growth hormone gene (GH) located on chromosome 19, growth hormone receptor gene (GHR) located on chromosome 11, progesterone receptor gene (PGR) located on chromosome 1, fibroblast growth factor gene (FGF) located on chromosome 3, insulin-like growth factor 1 and 2 genes (IGF1and IGF2) located on chromosome 4, myostatin gene (MSTN) located on chromosome 7 and melanocortin 4 receptor gene (MC4R) located on chromosome 9 have shown significant associations with body weighs in rabbits (Inoue et al, 2006;Fontanesi et al, 2008;Lu et al, 2011;Zhang et al, 2012;Fontanesi et al, 2012;Peng et al, 2013;Sahwan et al, 2014;Wu et al, 2015;Othman et al, 2015;El-Aksher et al, 2016;El-Sabrout and Aggag, 2017;Migdal et al, 2018). The growth hormone gene (GH) alters the metabolism of carbohydrates, proteins and lipids and promotes postnatal growth of mammals exerting direct or indirect effects on numerous tissues (Fontanesi et al, 2012).…”

Polymorphism of the melanocortin receptor gene and its association with egg production traits in Lohmann Brown chickens

“…HOLSINGER and WEIR (2009) revealed that the importance of discovery a large number of SNPs in the genomes from several species that has enabled exploration of genome-wide signatures in selection via an assessment of variation in marker allele frequencies among these populations. Genes associated with productive traits have been identified using single nucleotide polymorphisms of many candidate genes (ZHANG et al, 2013;WU et al, 2015). It is recognized that the egg production traits of chickens are controlled by a complicated multiple genes (GU et al, 2011).…”