Association of AdipoQ gene variation (rs1501299) and oxidative stress with cardiovascular disease in North West Indian population of Punjabi women

Abstract

Background: Till to date whether adiponectin AdipoQ gene variation (rs 1501299) is associated with cardiovascular disease, still remains controversial. Therefore, we aimed to relate the SNP (rs1501299) of adiponectin gene and oxidative stress in context to CVD in Punjabi women of North West India. Methods: In the present case-control study menopausal women with CVD as cases (n=265) and menopausal women without CVD as controls (n=258) were recruited. Genotyping of rs1501299 single nucleotide polymorphism of adiponectin gene was carried out by RFLP-PCR analysis. Biochemical parameters were analyzed according to the standard procedures. Results: Distribution of homozygous TT genotype of normolipidemic (p=0.001) and hyperlipidemic (p=0.001) women with CVD were significantly higher as compared to women without CVD. rs1501299 T allele carriers with CVD also showed significant (p=0.001) higher frequency distribution as compared to women without CVD. Under recessive model of inheritance TT mutant type homozygotes conferred ̴ 9 fold higher risk [p=0.001; OR= 9.60 (2.92-31.58)] towards CVD susceptibility for MDA>1.50; ̴ 11 fold higher risk [p=0.007; OR= 11.11 (1.49-82.83)] towards CVD for LDL carbonyl protein>15.04 and ̴ 9 fold higher risk [p=0.001; OR= 9.75 (2.30-41.22)] towards CVD susceptibility for SOD≤5.55. Under logistic regression analysis oxidative stress and TT genotype were significantly correlated with CVD. Conclusion: Our study revealed significant association of AdipoQ (rs1501299) gene polymorphism and oxidative stress with cardiovascular disease in Punjabi women of North West India. However, additional studies are required to support these findings. Keywords: AdipoQ gene, Association, Cardiovascular disease, MDA, RFLP-PCR, SOD

Adiponectin: A Promising Target for the Treatment of Diabetes and Its Complications

“…These SNPs within ADIPOQ play a crucial role in evaluating the link between common genetic variants, adiponectin levels, and the risk of diabetes. Notably, two common SNPs, rs2241766 and rs1501299, have shown significant correlations with type 1 diabetes mellitus, contributing to a distinct haplotype block [36][37][38][39][40][41].…”

Adiponectin gene polymorphisms associated with diabetes mellitus: A descriptive review

“…In fact, the pathogenesis of several deadly diseases including diabetic, neural and vascular disorders is significantly influenced by oxidative stress. In a recent study, adiponectin gene polymorphism AdipoQ (rs1501299) and oxidative stress were found to be significantly associated with cardiovascular disease [ 39 ]. Moreover, rs266729 and rs2241766 polymorphisms in adiponectin gene significantly enhanced the risk of cardiovascular disease [ 40 ].…”