Association of Alcaligenes faecalis with wetland rice

You, C. B.; Song, Wenwen; Wang, H. X.; Li, J. P.; Lin, Min; Hai, Weili

doi:10.1007/978-94-011-3486-6_34

Cited by 22 publications

(18 citation statements)

References 3 publications

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“…The diazotrophic strain A15 was isolated during surveys for nitrogen-fixing bacteria in the rhizosphere of paddy rice grown in China with suboptimal N fertilization (78). Yield increases of 3 to 7% have been reported for field-grown rice inoculated with strain A15 (77).…”

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confidence: 99%

Development and Application of a dapB -Based In Vivo Expression Technology System To Study Colonization of Rice by the Endophytic Nitrogen-Fixing Bacterium Pseudomonas stutzeri A15

Rediers

Bonnecarrère

Rainey

et al. 2003

Appl Environ Microbiol

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Pseudomonas stutzeri A15 is a nitrogen-fixing bacterium isolated from paddy rice. Strain A15 is able to colonize and infect rice roots. This strain may provide rice plants with fixed nitrogen and hence promote plant growth. In this article, we describe the use of dapB-based in vivo expression technology to identify P. stutzeri A15 genes that are specifically induced during colonization and infection (cii). We focused on the identification of P. stutzeri A15 genes that are switched on during rice root colonization and are switched off during free-living growth on synthetic medium. Several transcriptional fusions induced in the rice rhizosphere were isolated. Some of the corresponding genes are involved in the stress response, chemotaxis, metabolism, and global regulation, while others encode putative proteins with unknown functions or without significant homology to known proteins.Rice (Oryza sativa L.) is the staple food of over 40% of the world's population. Considering the increase in the world's population and the limited possibility to expand the acreage under cultivation, increasing the yield of rice production is of great concern. Yields in systems with a low input of N fertilizer can be increased considerably by a higher level of fertilization. However, the environmental concerns raised against the extensive use of fertilizers necessitate the search for alternatives. One of the explored alternatives is biofertilization through the interaction between nitrogen-fixing plant-growth-promoting rhizobacteria and rice.The diazotrophic strain A15 was isolated during surveys for nitrogen-fixing bacteria in the rhizosphere of paddy rice grown in China with suboptimal N fertilization (78). Yield increases of 3 to 7% have been reported for field-grown rice inoculated with strain A15 (77). Initially, this strain was phenotypically characterized as Alcaligenes faecalis, but the taxonomic position of strain A15 was reinvestigated, and it was reclassified as Pseudomonas stutzeri (69). It has been shown that the strain A15 nitrogen fixation genes (nif) are expressed in the rice rhizosphere (68). Since strain A15 is able to infect rice roots and survive within rice plants (24,67,79), it may provide rice plants with fixed nitrogen and hence promote plant growth. However, direct evidence for bacterial N transfer to the plants is still lacking.At present, the mechanisms that enable strain A15 to colonize and infect rice roots and survive within rice plants are not known. The lack of a readily scored plant phenotype has hampered the identification and characterization of the P. stutzeri A15 genes that are required for interaction with the host plant. Because conditions during bacterium-host interactions are difficult to mimic in vitro, new techniques have been devised to study in vivo gene expression; these include differential fluorescence induction, signature-tagged mutagenesis, RNA arbitrarily primed PCR, and in vivo expression technology (IVET) (reviewed in references 8, 22, and 51). In this study, an IVET strategy was dev...

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confidence: 99%

Development and Application of a dapB -Based In Vivo Expression Technology System To Study Colonization of Rice by the Endophytic Nitrogen-Fixing Bacterium Pseudomonas stutzeri A15

Rediers

Bonnecarrère

Rainey

et al. 2003

Appl Environ Microbiol

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“…The range of expression of Ni resistance was examined for all mini-Tn5 Ni resistance transposons. To do this, the pUT-based constructs were introduced into E. coli S17-1 (pir) (5) and subsequently transferred by conjugation into the nickel-sensitive strains R. eutropha AE104 (12), E. coli DH10B, Burkholderia cepacia W1.2 (isolated from wheat) and LS2.4 (isolated from lupine shoots) (a gift from K. Ophel-Keller), Herbaspirillum seropedicae LMG2284 (associated with rye grass) (1), Pseudomonas stutzeri A15 (associated with rice roots) (13,25), Azospirillum irakense KBC1 (a rice endophyte) (9), and Pseudomonas putida VMO433. The last strain was isolated as an endophytic bacterium after surface sterilization of Brassica napus plants (Lodewyckx and van der Lelie, unpublished data).…”

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confidence: 99%

Nickel-Resistance-Based Minitransposons: New Tools for Genetic Manipulation of Environmental Bacteria

Taghavi

Delanghe

Lodewyckx

et al. 2001

Appl Environ Microbiol

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The ncc and nre nickel resistance determinants from Ralstonia eutropha-like strain 31A were used to construct mini-Tn5 transposons. Broad host expression of nickel resistance was observed for the nre minitransposons in members of the ␣, ␤, and ␥ subclasses of the Proteobacteria, while the ncc minitransposons expressed nickel resistance only in R. eutropha-like strains.Several nickel resistance determinants have been identified in Ralstonia eutropha (Alcaligenes eutrophus) (24) strains isolated from different biotopes heavily polluted with heavy metals. The cnrYXHCBA operon of R. eutropha CH34 plasmid pMOL28 (12), which mediates medium levels of nickel resistance (up to 10 mM) and cobalt resistance, is the most thoroughly studied determinant (3,11,17,18,20). The resistance mechanism mediated by cnr is inducible and is due to an energy-dependent efflux system driven by a chemo-osmotic proton-antiporter system (6,18,22,23). A 14.5-kb BamHI fragment of plasmid pTOM9 from R. eutropha-like strain 31A (Alcaligenes xylosoxidans 31A) (10) and a similar BamHI fragment of plasmid pGOE2 from R. eutropha-like strain KTO2 were also found to encode Ni resistance. On both fragments a locus mediating high-level nickel resistance (up to 20 to 50 mM) and a locus mediating low-level nickel resistance (3 mM) were identified and designated ncc and nre, respectively (15, 16). The nccYXHCBAN determinant, which except for the nccN gene is very similar to cnr, causes high levels of nickel and cobalt resistance and a low level of cadmium resistance in R. eutropha. Neither cnr nor ncc is expressed in Escherichia coli. On the other hand, the 1.8-kb nre locus causes low levels of nickel resistance in both Ralstonia and E. coli (16). An nre-like determinant, which could be expressed in E. coli and Citrobacter freundii, was also found in Klebsiella oxytoca CCUG15788 (19, 20).Recently, amplified ribosomal DNA restriction analysis was used to determine the phylogenetic position of zinc-and nickel-resistant Ralstonia-like strains (2). The ncc operon was found in many nickel-resistant R. eutropha-like strains and in environmental strains in the direct vicinity of the genus Burkholderia (2), a member of the ␤ subclass of the class Proteobacteria like the genus Ralstonia. This might indicate that ncc has range of expression broader than the genus Ralstonia.Heavy metal resistance markers with broad host expression ranges have been shown to be useful for genetic manipulation of Pseudomonas strains potentially designated for environmental release (14). Broad-host-range expression of ncc-nre was recently confirmed by Dong et al. (7), who found ncc-nre-based Ni resistance in Comamonas, Sphingobacterium heparinum, flavobacteria, and even gram-positive bacteria related to Arthrobacter. However, it was not clear from this study which of the Ni resistance determinants was responsible for the broadhost-range Ni resistance. In addition, plasmid instability problems were encountered with some of the transconjugants. In order to study the range of expression of ...

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“…There are few reports, instead, on the intracellular presence of endophytes. This seems to be an occasional finding, except for particular cases such as Rhizobium or Alcaligenes faecalis (You et al, 1991) where both bacteria are enveloped by the plant in specialised structures. Other microorganisms can easily reach xylematic vessels passing through the cortical layer and root endodermis.…”

Section: Endophytes and Their Hypothetical Rolementioning

confidence: 94%

Endophytic bacteria: their possible role in the host plant

Fiore

Gallo

1995

Azospirillum VI and Related Microorganisms

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The presence of bacteria in the internal tissues of healthy plants has largely been ignored in the past, although in the last fifty years more and more isolations of microorganisms from plant roots, leaves, stems and even spikes and seeds, have been reported. Little is known about their biological role inside the host plants. Up to now endophytes have given rise to a new biological studies, as they can be employed as plant protecting or plant growth-promoting agents and as nitrogen fixers to supply the associated plant with nitrogen. The aim of this paper is to compare different approaches to the study of endophytes and to formulate a hypothesis about their role. We maintain that they are mostly a natural associative component of the plant micro-ecosystem, able to balance and to enhance their host fitness to the surrounding environment. First results about their applications in agriculture are discussed.

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Association of Alcaligenes faecalis with wetland rice

Cited by 22 publications

References 3 publications

Development and Application of a dapB -Based In Vivo Expression Technology System To Study Colonization of Rice by the Endophytic Nitrogen-Fixing Bacterium Pseudomonas stutzeri A15

Development and Application of a dapB -Based In Vivo Expression Technology System To Study Colonization of Rice by the Endophytic Nitrogen-Fixing Bacterium Pseudomonas stutzeri A15

Nickel-Resistance-Based Minitransposons: New Tools for Genetic Manipulation of Environmental Bacteria

Endophytic bacteria: their possible role in the host plant

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