Association of &amp;lt;i&amp;gt;β&amp;lt;/i&amp;gt;-casein gene polymorphism with milk composition traits of Egyptian Maghrebi camels (&amp;lt;i&amp;gt;Camelus dromedarius&amp;lt;/i&amp;gt;)

Nowier, Amira M.; Ramadan, Sherif

doi:10.5194/aab-63-493-2020

Cited by 12 publications

(6 citation statements)

References 35 publications

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“…One ecotype, Ardhaoui Medenine, deviated from the aver-age CSN2 G allele frequency (0.550 vs. 0.462) of the total population, whereas in the Abidi camels, the 2 alleles (0.50) were perfectly balanced. Although the sample size was small and similar in both populations, this result was at odds with findings from studies of a similarly-sized population (Pauciullo et al, 2014;Nowier and Ramadan, 2020). The β-CN protein is both abundant and important to camel milk processing.…”

Section: Discussioncontrasting

confidence: 57%

“…The β-CN protein is both abundant and important to camel milk processing. Nowier and Ramadan (2020) proposed an association study of β-CN and milk chemical composition traits in Maghrebi camel breeds because high levels of the protein and acidity in A/A-genotyped individuals suggest an affinity for cheese processing. Our data confirmed that allele A is more frequent in the Tunisian popula- Different superscripts mean significant differences (P < 0.05).…”

Section: Discussionmentioning

confidence: 99%

“…A recent study that fully described the casein gene cluster in dromedary camel revealed the assembly of the 4 genes in a DNA fragment of about 190 kb (Pauciullo et al, 2019). Deciphering the structure and polymorphism in the casein gene in the camel species allowed researchers to investigate casein gene variability in different camel populations (Othman et al, 2016;Yelubayeva et al, 2018;Redwan et al, 2018;Nowier and Ramadan, 2020). Nevertheless, to our knowledge, no studies have been conducted in Tunisia where camel population includes roughly 80,000 productive females per 2017 statistics produced by the Office d'Elevage et de Pâturage (OEP, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Tunisian camel casein gene characterization reveals similarities and differences with Sudanese and Nigerian populations

Letaief

Bedhiaf-Romdhani

Salem³

et al. 2022

Journal of Dairy Science

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Milk is a primary protein source that has always played a role in mammalian health. Despite the intensification of research projects on dromedary and the knowledge of the genetic diversity at the casein loci, the genetic structure of the Tunisian camel population still needs exploration. This study sought to determine the genetic diversity of 3 casein gene variants in 5 Tunisian camel ecotypes: c.150G>T at CSN1S1 (α S1 -casein), g.2126A>G at CSN2 (β-casein), and g.1029T>C at CSN3 (κ-casein). The obtained results were compared with data published on Sudanese and Nigerian camels to establish the level of differentiation within and between populations. A total of 159 blood samples were collected from 5 Tunisian camel ecotypes and the extracted DNA was genotyped by PCR-RFLP. A streamlined genotyping protocol was also developed for CSN3. Results indicated that allele T was quite rare (0.06) at CSN1S1 for all ecotypes. Minor allele frequency was found for G (0.462) in CSN2 except for Ardhaoui Medenine ecotype who deviated from the average CSN2 allele frequency of the total population. Allele C showed minor allele frequency of 0.384 in CSN3. Among the Tunisian population, GAT (0.343) was the most represented haplotype in all ecotypes except for Ardhaoui Medenine, where GGC (0.322) was the most frequent one. Significant differences in heterozygosity and local inbreeding were observed across the Tunisian, Sudanese, and Nigerian populations, although the global fixation index indicated that only 2.2% of the genetic variance is related to ecotype differences. Instead, phylogenetic analysis revealed a closer link between the Tunisian and Sudanese populations through a clade subdivision with 3 main branches among the ecotypes.This study represents the first attempt to understand casein gene variability in Tunisian camels; with further study, milk traits and genetic differentiation among populations can be associated with the history of camel domestication.

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Section: Discussioncontrasting

confidence: 57%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Tunisian camel casein gene characterization reveals similarities and differences with Sudanese and Nigerian populations

Letaief

Bedhiaf-Romdhani

Salem³

et al. 2022

Journal of Dairy Science

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“…One such gene is the K-CN gene, which has been studied for its polymorphisms and their effects on milk traits and properties [12]. Another gene involved in MAS is the β-casein (CSN2) gene, which has a specific SNP (2126 A/G) that has been associated with milk composition traits in Maghrebi camels [13]. Additionally, the alphalactalbumin gene has been studied for its genetic polymorphisms and their relationship with milk protein concentration in Maghrebi camels.…”

Section: Marker Assisted Selectionmentioning

confidence: 99%

Polymorphism Analysis of DGAT1 Gene and Novel Association Studies with Milk Production Traits in Domestic Dromedary Camel in India

Harsh,

Pannu,

Prakash

et al. 2024

UPJOZ

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Several polymorphisms related to different gene loci have been proven to affect milk production traits and milk composition in camel. The current investigation was carried out in order to determine the genetic polymorphism of the DGAT1 gene and its association with milk production traits in camels. The SNP analysis focused on a 773 bp long fragment of the DGAT1 gene that was amplified using specific primer pairs through PCR. The Novel dromedary sequencing results revealed partial exon-6,10; complete exon-7, 8, and 9 as well as complete intron-6, 7, 8, and 9 in the 773 bp DNA fragment. A single nucleotide variation was identified at position 463 C>T of exon-8 in both Bikaneri and Kachchhi breeds. Association studies of genotypes of DGAT1 gene (CC and CT) with milk production and composition traits were examined using GLM in SPSS. The effect of breed on lactation yield and lactation length was found non-significant (P>0.05) and the effect of breed on peak yield was found highly significant (P≤0.01) and on average daily milk yield was found significant (P≤0.05). Effect of genotype, year of calving, age of calving and parity on lactation length, lactation yield, peak yield and average daily milk yield were found non-significant (P>0.05).

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“…A genetic variation in kappa-casein may also indicate that the mature protein is associated with other polymorphisms in non-coding sequences (promoter). A mutation could cause some alleles to express differently, such as the two main alleles of bovine cattle's kappa-casein gene (30) .…”

Section: Observed Genotypesmentioning

confidence: 99%

Association of Kappa casein gene polymorphism with milk production traits in crossbred dairy cows

et al. 2023

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Milk’s qualitative and technological properties are greatly affected by genetic polymorphisms in the kappa-casein gene, and their polymorphisms may serve as informative markers of yield and composition. Thus, the objective of this study was to detect kappa-casein (kappa-CN) gene polymorphisms and their association with milk production traits in crossbred dairy cows. One hundred healthy crossbred (Friesian x Jenoubi) dairy animals between three and five years old were sampled for blood and milk during their first lactation. The genomic DNA was extracted from whole blood, and restriction fragment length polymorphism (RFLP-PCR) was used to determine the genotype of the kappa-CN gene. As a consequence of the restriction digestion of this fragment with Hind III, it showed three different restriction patterns: BB (453 base pairs uncut), AB (453, 206, and 225 base pairs), and AA (206 and 225 base pairs). Based on genetic diversity, the AB genotype was the most predominant (n = 67), with a frequency of 0.67. A variant genotype of the kappa-CN gene was associated with milk production traits in crossbred dairy cows. Animals with the AA variant produced a higher milk yield and a higher percentage of fat, casein, protein, and solids not fat (SNF) (P≤0.05) (1.397kg, 0.75%, 0.31%, 0.27%, and 0.68%, respectively) than those with the BB variant. A logistic regression analysis confirmed that the kappa-CN genotypes increase milk yield and casein content. Therefore, genetic variants of the kappa-CN gene could be used as genetic markers for improving milk production traits in dairy cattle.

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Association of <i>β</i>-casein gene polymorphism with milk composition traits of Egyptian Maghrebi camels (<i>Camelus dromedarius</i>)

Cited by 12 publications

References 35 publications

Tunisian camel casein gene characterization reveals similarities and differences with Sudanese and Nigerian populations

Tunisian camel casein gene characterization reveals similarities and differences with Sudanese and Nigerian populations

Polymorphism Analysis of DGAT1 Gene and Novel Association Studies with Milk Production Traits in Domestic Dromedary Camel in India

Association of Kappa casein gene polymorphism with milk production traits in crossbred dairy cows

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