Association of an isozyme locus and strawbreaker foot rot resistance derived from Aegilops ventricosa in wheat

McMillin, David E.; Allan, R. E.; Roberts, Diana

doi:10.1007/bf00266539

Cited by 58 publications

(44 citation statements)

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“…Close association between Pch1 and a codominant endopeptidase marker allele, Ep-D1b, was demonstrated by McMillin et al (1986) and subsequently used to monitor introgression of eyespotresistance (Pch1) from VPM-1 to elite lines. Improvements in the endopeptidase assay have recently been published and this assay was found to be completely accurate in predicting resistance (Santra et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Identification of a candidate gene for the wheat endopeptidase Ep-D1 locus and two other STS markers linked to the eyespot resistance gene Pch1

et al. 2007

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Hansen, Jennifer L.; Zemetra, Robert S.; Santra, Dipak K.; Campbell, Kimberly G.; and Riera-Lizarazu, Oscar, "Identification of a candidate gene for the wheat endopeptidase Ep-D1 locus and two other STS markers linked to the eyespot resistance gene Pch1" (2008 Abstract Wheat is prone to strawbreaker foot rot (eyespot), a fungal disease caused by Oculimacula yallundae and O. acuformis. The most eVective source of genetic resistance is Pch1, a gene derived from Aegilops ventricosa. The endopeptidase isozyme marker allele Ep-D1b, linked to Pch1, has been shown to be more eVective for tracking resistance than DNA-based markers developed to date. Therefore, we sought to identify a candidate gene for Ep-D1 as a basis for a DNA-based marker. Comparative mapping suggested that the endopeptidase loci Ep-D1 (wheat), enp1 (maize), and Enp (rice) were orthologous. Since the product of the maize endopeptidase locus enp1 has been shown to exhibit biochemical properties similar to oligopeptidase B puriWed from E. coli, we reasoned that Ep-D1 may also encode an oligopeptidase B. Consistent with this hypothesis, a sequence-tagged-site (STS) marker, Xorw1, derived from an oligopeptidase B-encoding wheat expressed-sequence-tag (EST) showed complete linkage with Ep-D1 and Pch1 in a population of 254 recombinant inbred lines (RILs) derived from a cross between wheat cultivars Coda and Brundage. Two other STS markers, Xorw5 and Xorw6, and three microsatellite markers (Xwmc14, Xbarc97, and Xcfd175) were also completely linked to Pch1. On the other hand, Xwmc14, Xbarc97, and Xcfd175 showed recombination in the W7984 £ Opata85 RIL population suggesting that recombination near Pch1 is reduced in the Coda/Brundage population. In a panel of 44 wheat varieties with known eyespot reactions, Xorw1, Xorw5, and Xorw6 were 100% accurate in predicting the presence or absence of Pch1 whereas Xwmc14, Xbarc97, and Xcfd175 were less eVective. Thus, linkage mapping and a germplasm survey suggest that the STS markers identiWed here should be useful for indirect selection of Pch1.

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Section: Introductionmentioning

confidence: 99%

Identification of a candidate gene for the wheat endopeptidase Ep-D1 locus and two other STS markers linked to the eyespot resistance gene Pch1

et al. 2007

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“…(Maia 1967) and is located on the distal end of chromosome 7D (Doussinault et al 1983;Worland et al 1988). According to McMillin et al (1986), there is a close association between the Pch1 resistance gene and EP-D1b, an endopeptidase isozyme allele from Ae. ventricosa chromosome 7DL.…”

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confidence: 99%

“…jic-5A loci) and negative (Ozon and Patras, eyespot susceptible) control varieties. Pch1, Pch2 and Q.Pch.jic-5A loci identification was made using eight verified markers (Table 1) according to McMillin et al (1986); Groenewald et al (2003); Chapman et al (2008) and Burt et al (2011). Based on the marker analysis, we selected five groups of breeding lines, carrying: (1) the Pch1 gene alone: one line; (2) the Pch2 gene alone: four lines; (3) the Q.Pch.jic-5A alone: one line; and (4) Pch1 + Q.Pch.jic-5A: three lines.…”

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confidence: 99%

How do eyespot resistance genes transferred into winter wheat breeding lines affect their yield?

Kwiatek

Wiśniewska

Korbas

et al. 2016

Journal of Plant Protection Research

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Eyespot can reduce yields, even up to 50%. There are four genetically characterized resistances in wheat varieties, controlled by: (1) the Pch1 gene, transferred from Aegilops ventricosa; (2) the Pch2 gene, originating from wheat variety Capelle Desprez; (3) the Q.Pch.jic-5A genes was compared with resistance conferred by Pch1 or Q.Pch.jic-5A alone. We found significant differences between infection scores evaluated in resistant lines carrying Pch1 and Q.Pch.jic-5A alone, while no differences in terms of the level of resistance expression were detected between Pch1 alone and Pch1 + Q.Pch.jic-5A, and between wheat lines carrying Pch1 and Pch2 alone. Moreover, we demonstrated that the Pch1 gene, together with an Ae. ventricosa segment, caused statistically significant yield losses, both as a single eyespot resistance source or in a combination with Q.Pch.jic-5A. Yield scores showed that wheat lines with Q.Pch.jic-5A had the highest yields, similar to the yielding potential of Pch2-bearing lines and control varieties.

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“…"Marne" (Maia 1967 (Doussinault et al 1983). The transferred sequence with the Pch1 gene has been mapped in chromosome arm 7DL (Jahier et al 1978;McMillin et al 1986;Worland et al 1988). "VPM1" line was a base for breeders to create new resistance cultivars like "Rendezvous" (used in the presented work as a resistance standard).…”

Section: Introductionmentioning

confidence: 99%

“…An identification of the Pch1 locus is provided by closely linked endopeptidase gene locus EpD1b (McMillin et al 1986;Worland et al 1988;Groenewald et al 2003). Endopeptidase-1 (Ep-1) in wheat is controlled by 3 loci: EpA1, EpB1 and EpD1, located in homoeologous chromosomes 7AL, 7BL and 7DL, respectively (Hart and Langston 1977;Tuleen 1977, Koebner et al 1988;Santra et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Using markers and field evaluation to identify the source of eyespot resistance genePch1in the collection of wheat breeding lines

Kwiatek

Wiśniewska

Kaczmarek

et al. 2015

Cereal Research Communications

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Pch1 gene translocated from Aegilops ventricosa provides effective resistance to eyespot in wheat. To track the Pch1 gene introgression, we investigated 372 genotypes obtained from various breeding programs using endopeptidase EpD1b marker, sequence-tagged-site (STS) marker XustSSR2001-7DL, and the score of infection index (K-index) evaluated after in vivo inoculation test. These genotypes were divided into three groups with 136, 124 and 112 genotypes for the field test lasting three years. In 2011, the mean K-index was 0.81, while 2012 and 2013 the mean K-indexes were 1.60 and 1.46, respectively. Both marker results indicated that 18 genotypes possessed Pch1 gene. Statistical analysis of the level of K-index showed that these 18 genotypes were resistant to eyespot, which verified the proper assignment of wheat genotypes with Pch1 gene based on the marker data. Thus, the endopeptidase and XustSSR2001-7DL are useful for identifying sources of eyespot resistance gene Pch1 in wheat breeding program.

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Association of an isozyme locus and strawbreaker foot rot resistance derived from Aegilops ventricosa in wheat

Cited by 58 publications

References 6 publications

Identification of a candidate gene for the wheat endopeptidase Ep-D1 locus and two other STS markers linked to the eyespot resistance gene Pch1

Identification of a candidate gene for the wheat endopeptidase Ep-D1 locus and two other STS markers linked to the eyespot resistance gene Pch1

How do eyespot resistance genes transferred into winter wheat breeding lines affect their yield?

Using markers and field evaluation to identify the source of eyespot resistance genePch1in the collection of wheat breeding lines

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