Association of ATP1A1 gene polymorphism with thermotolerance in Tharparkar and Vrindavani cattle

Abstract

Aim:One of the major biochemical aspects of thermoregulation is equilibrium of ion gradient across biological membranes. Na+/K+-ATPase, a member of P type-ATPase family, is a major contributor to the mechanism that actively controls cross-membrane ion gradient. Thus, we examined ATP1A1 gene that encodes alpha-1 chain of Na+/K+-ATPase, for genetic polymorphisms.Materials and Methods:A total of 100 Vrindavani (composite cross strain of Hariana x Holstein-Friesian/Brown Swiss/Jersey) and 64 Tharparkar (indigenous) cattle were screened for genetic polymorphism in ATP1A1 gene, using polymerase chain reaction single-strand conformation polymorphism and DNA sequencing. For association studies, rectal temperature (RT) and respiration rate (RR) of all animals were recorded twice daily for 3 seasons.Results:A SNP (C2789A) was identified in exon 17 of ATP1A1 gene. Three genotypes namely CC, CA, and AA were observed in both, Vrindavani and Tharparkar cattle. The gene frequencies in Tharparkar and Vrindavani for allele A were 0.51 and 0.48, and for allele C were 0.49 and 0.52, respectively, which remained at intermediate range. Association study of genotypes with RT and RR in both cattle population revealed that the animals with genotype CC exhibited significantly lower RT and higher heat tolerance coefficient than CA and AA genotypes.Conclusion:Differential thermoregulation between different genotypes of ATP1A1 gene indicate that the ATP1A1 gene could be potentially contributing to thermotolerance in both, Tharparkar, an indigenous breed and Vrindavani, a composite crossbred cattle.

Review: Adaptation of animals to heat stress

“…The genotypic outcomes of blood ATP1A1 gene shows an association with various heat tolerance variables including RR and RT in both Tharparkar and Vrindavani cattle breeds (Kashyap et al, 2015). Moreover, higher expression of blood ATP1A1 gene was also reported in cattle breeds during summer, which also indicates its potential role for counteracting heat stress in cattle (Kashyap et al, 2015). Further, modified hepatic expression patterns of NADH dehydrogenase gene were also reported in cattle during heat stress exposure (Koch et al, 2016).…”

“…The internal metabolic heat production of stressed animals is reduced via hypothyroid activity (Weitzel et al, 2017). Studies suggest the potential of the bovine blood ATP1A1 gene in ameliorating the effects of heat stress, and the P14 locus within the bovine ATP1A1 gene has been recognized as a DNA marker for bovine heat tolerance in marker-assisted selection (Kashyap et al, 2015). The genotypic outcomes of blood ATP1A1 gene shows an association with various heat tolerance variables including RR and RT in both Tharparkar and Vrindavani cattle breeds (Kashyap et al, 2015).…”

“…Studies suggest the potential of the bovine blood ATP1A1 gene in ameliorating the effects of heat stress, and the P14 locus within the bovine ATP1A1 gene has been recognized as a DNA marker for bovine heat tolerance in marker-assisted selection (Kashyap et al, 2015). The genotypic outcomes of blood ATP1A1 gene shows an association with various heat tolerance variables including RR and RT in both Tharparkar and Vrindavani cattle breeds (Kashyap et al, 2015). Moreover, higher expression of blood ATP1A1 gene was also reported in cattle breeds during summer, which also indicates its potential role for counteracting heat stress in cattle (Kashyap et al, 2015).…”

Single nucleotide polymorphisms in ATP1A1 gene and their association with thermotolerance traits in Sahiwal and Karan Fries cattle

“…Identification of SNPs in these stress responsible genes and their association with variation in animal sensitivity to thermal stress will permit screening of animals having presence or absence of desirable or undesirable alleles (Collier et al, 2008). Na + , K + -ATPase 1-subunit gene (ATP1A1) polymorphisms were associated with heat (Liu et al, 2010;Liu et al, 2011), Murrah Buffalo (Jayakumar, 2014), Jersey crossbred cows (Das et al, 2015) and in Tharparkar and Vrindavani crossbred cattle (Kashyap, 2015). In our study, all possible three genotypes (homozygous and heterozygous) at each SNP locus were present in Sahiwal cows.…”

Genetic Parameters and Genome-Wide Association Studies for Anti-Müllerian Hormone Levels and Antral Follicle Populations Measured After Estrus Synchronization in Nellore Cattle

“…In this regard, ovulation was suggested to be a tissue remodeling process, annotated as a significant biological process term (p = 0.02). The genes CNTFR and ATP1A1 are associated with productive efficiency (e.g., average daily gain, feed efficiency) [74] and heat tolerance [75][76][77] indicators. Selection for fertility along with production traits is, therefore, readily justifiable.…”