Association of autoantibodies against the phosphatidylserine–prothrombin complex with manifestations of the antiphospholipid syndrome and with the presence of lupus anticoagulant

Abstract

Antiprothrombin antibodies are heterogeneous and their clinical relevance depends on the method of detection applied. Positive results on the aPS/PT test can serve as a marker of thrombotic events in patients with autoimmune diseases.

Specificities, properties, and clinical significance of antiprothrombin antibodies

“…We have reported the detection of APS/PT and have shown that the results obtained depend closely on the assay performed (37). The presence of APS/PT, but not APT-A, correlated significantly with the clinical manifestations of antiphospholipid syndrome (OR 8.29, 95% CI 3.03-22.71, P Ͻ 0.0001 versus OR 1.89, 95% CI 0.71-5.06, P not significant).…”

“…Our group (37) recently reported that the detection of APS/PT strongly correlated with the presence of LAC. Most investigators have used only the APT-A ELISA, presuming that both systems of detection (APT-A and APS/PT ELISAs) detect the same antibodies.…”

“…Recently, Simmelink et al (40) showed that addition of affinity-purified APTs from LAC-positive plasma to normal plasma induced LAC activity, and that LAC (25) Plasma coagulation disturbances in SLE could result from a combination of a plasma inhibitor and hypoprothrombinemia Bajaj et al, 1983 (26) Presence of prothrombin-binding antibodies in patients with LAC and hypoprothrombinemia Edson et al, 1984 (27) Prothrombin-APT complexes demonstrated by CIE in the plasma of patients with LAC and without severe hypoprothrombinemia Fleck et al, 1988 (28) Circulating prothrombin-APT complexes observed in patients with LAC and normal PT levels Bevers et al, 1991 (8) Importance of APT in causing LAC activity Oosting et al, 1993 (11) LAC inhibited endothelial cell-mediated prothrombinase activity; IgG fraction containing LAC activity bound to phospholipidprothrombin complex Arvieux et al, 1995 (35) APT could be detected by a standard ELISA (APT-A ELISA) Matsuda et al, 1996 (36) APS/PT could be detected in LAC-positive patients Galli et al, 1997 (30) APT recognized prothrombin more efficiently in ELISA using PSbound prothrombin Atsumi et al, 2000 (37) Strong correlation between APS/PT and antiphospholipid syndrome * LAC ϭ lupus anticoagulant; SLE ϭ systemic lupus erythematosus; CIE ϭ counter immunoelectrophoresis; ELISA ϭ enzyme-linked immunosorbent assay; APT-A ϭ antibodies against prothrombin alone; APS/PT ϭ antibodies against phosphatidylserine-prothrombin complex.…”

Study of Phosphatidylserine-dependent Anti-prothrombin Antibody in Cerebral Infarction

“…Recently, autoantibodies for prothrombin as well as beta 2-GPI aCL have been shown to be associated with LA (1,2), and IgG phosphatidylserine-dependent antiprothrombin antibodies (aPS/PT) were also reported to be strongly associated with LA (3) T a b l e 1 . B a s e l i n e Ch a r a c t e r i s t i c s a n d F e a t u r e s o …”

Antiphospholipid antibodies in patients with autoimmune blistering disease

“…The serological markers for this syndrome are antiphospholipid antibodies (aPLs), such as the lupus anticoagulant (LA), anti-cardiolipin antibody (aCL) 5,6 , antibody against anticardiolipin β 2 -glycoprotein I complex antibody (CL/β 2 GPI), and anti-phosphatidylserine-prothrombin complex antibody (aPS/PT) 7,8 . aPLs are frequently found in patients with systemic autoimmune diseases, especially in systemic lupus erythematosus (SLE).…”