Association of C3953T transition in interleukin 1β gene with idiopathic male infertility in an Iranian population

Abstract

In this study we investigate the association of C3953T transition single nucleotide polymorphism in the fifth exon of the interleukin 1β gene with idiopathic male infertility. In a case-control study, blood samples were collected from 230 fertile and 207 infertile men who referred to the Kashan IVF centre. Genotypes of samples at the C3953T location were determined by polymerase chain reaction-restriction fragment length polymorphism. The data showed a significant association of TT genotype (OR = 2.49, 95%CI = 1.02-6.10; p = 0.0452) and T allele (OR = 1.46, 95%CI = 1.07-1.99; p = 0.0174) with male infertility. In a subgroup analysis, we found that the TT genotype (OR = 3.28, 95%CI = 1.16-9.26; p = 0.0249) and T allele (OR = 1.63, 95%CI = 1.10-2.41; p = 0.0142) were associated with oligozoospermia. Our findings suggest that the C3953T polymorphism could be considered as a potential biomarker for a genetic diagnosis of male infertility.

IL-1RA VNTR and IL-1α 4845G>T polymorphisms and risk of idiopathic male infertility in Iranian men: A case-control study and an in silico analysis

“…Genetic polymorphisms can alter gene expression, RNA structure and/or protein function depending on their gene location (Karimian & Hosseinzadeh Colagar, ; Mazaheri, Karimian, Behjati, Raygan, & Colagar, ; Zamani‐Badi, Karimian, Azami‐Tameh, & Nikzad, ). While in vitro and in vivo analyses are useful for detecting the effects of polymorphisms on molecular aspects of the origin gene, they are costly and time consuming.…”

Association analysis of rs1695 and rs1138272 variations in GSTP1 gene and breast cancer susceptibility

“…Genetic variations based on their positions in a gene could alter the gene function (Salimi et al, 2017;Nejati et al, 2018). The SNPs on the promoter of a gene may alter the gene expression however the SNPs in the intron regions could alter the production of mature mRNA by interfering with the splicing process (Mobasseri et al, 2019;Zamani-Badi et al, 2019). But, the missense mutations could alter the structure and function of proteins (Noureddini et al, 2018;Bafrani et al, 2019) what may be true for GSTP1-Ile105Val genetic variation.…”

Arg399Gln substitution in XRCC1 as a prognostic and predictive biomarker for prostate cancer: Evidence from 8662 subjects and a structural analysis

“…According to our previous studies and to clarify the molecular aspects of Arg399Gln substitution on the XRCC1 protein, we used bioinformatics tools . In silico analysis provides a time‐ and cost‐consuming way of analyzing coding and non‐coding SNPs . Our bioinformatics analysis indicated that the Arg399Gln polymorphism is a damaging variation.…”