Association of CAT–262C/T with the concentration of catalase in seminal plasma and the risk for male infertility in Algeria

Abstract

Catalase (CAT) plays a central role in the protection of different cell types against the deleterious effects of hydrogen peroxide. In human, CAT is implicated in many physiological and pathological conditions including idiopathic male infertility. In this study we examined the association between CAT levels in seminal plasma with different sperm parameters and with CAT-262 C/T polymorphism and their risk for idiopathic male infertility in Algeria. Semen and blood samples were obtained from 111 infertile males and 104 fertile controls from the region of Eastern Algeria following informed consent. Standard semen parameters, DNA integrity, and CAT concentration in seminal plasma were evaluated. CAT-262C/T genotypes were screened using allele specific PCR. Seminal CAT activity was significantly different (p<0.0001) between infertile males and controls, it was also markedly decreased in oligo-astheno-teratozoospermia (p<0.0001), azoospermia (p<0.0001), and normozoospermia (p=0.045) subgroups compared to controls. Positive correlations between CAT activity and semen parameters (volume, motility, concentration, and morphology) were detected, but not with sperm DNA integrity. There was no direct association between CAT-262C/T polymorphism and general male infertility. However, the results presented in this study showed that CAT activity is remarkably associated with the CAT-262T allele (p=0.001) and the different CAT-262C/T genotypes. This study highlighted the major differences in the seminal plasma CAT content between infertile and fertile males and the differences of CAT concentration between different CAT-262C/T genotypes carriers.

Oxidative stress and phenotype frequencies of paraoxonase‐1 in teratozoospermia

“…Shiva et al () indicated higher catalase and thiol levels in the group with high sperm counts compared to the group with low sperm counts. Bousnane, May, Yahia, and Abu Alhaija () found that catalase levels decreased in groups with azoospermia and oligoasthenoteratozoospermia. However, contrary to our findings, Zini and Libman () reported that in catalase‐like activities between fertile and infertile males, and Tavilani, Goodarzi, Vaisi‐Raygani, Salimi, and Hassanzadeh () showed no significant difference in catalase concentrations in seminal plasma between asthenozoospermic individuals and controls.…”

“…In their correlation studies, Bousnane et al () showed that there was a positive correlation between the catalase concentration in the seminal plasma of infertile men with the most important criteria that give normal fertilisation ability to spermatozoa: sperm concentration ( p < 0.0001, r = 0.348), and sperm motility ( p < 0.0001, r = 0.585) and the percentage of typical sperm morphology ( p < 0.0001, r = 0.599). In the present study, we found out that sperm morphology was positively correlated with catalase and thiol levels.…”

Carnosine treatment during human semen processing by discontinuous density gradient

“…Most reports describe that antioxidant supplementation in semen may improve the oxidative equilibrium (Bousnane, May, Yahia, & Abu‐Alhaija, ). This has been attempted during cryopreservation (Panner Selvam, Henkel, Sharma, & Agarwal, ; Satorre, Breininger, & Beconi, ), in vitro fertilisation (Griveau & Le Lannou, ) and semen processing (Hughes, Lewis, McKelvey‐Martin, & Thompson, ; Raad et al, ), to name a few.…”

Tri-nucleotide consortium of androgen receptor is associated with low serum FSH and testosterone in asthenospermic men

“…Structurally, the AR protein has three distinct functional domains: i) a hypervariable N-terminal highly flexible transactivation domain (NTD) that comprises approximately 60% of AR protein [Mostaghel et al 2014]; ii) a highly conserved central DNA-binding domain (DBD); and iii) a hinge region (HR) and C-terminal ligand binding domain (LBD). Plasticity and flexibility are two major characteristics of the LBD [Bousnane et al 2017]. This cofactor binding groove provides a ligand induced protein-protein interaction surface that has an ability to interact with the FxxLF motif of NTD.…”