Association of Coronary Heart Disease with Pre-β-HDL Concentrations in Japanese Men

Abstract

Background:In individuals heterozygous for ABCA1 transporter mutations, defective reverse cholesterol transport (RCT) causes low HDL-cholesterol and premature coronary heart disease (CHD). However, the extent to which impaired RCT underlies premature CHD in others with low HDL-cholesterol is not known. The primary acceptors of cell cholesterol are a minor subclass of lipid-poor pre-␤-HDLs. These are generated during remodeling of ␣-HDLs, which account for almost all HDL-cholesterol. We studied the strength of the association of CHD with pre-␤-HDL concentrations in Japanese men. Methods: Blood was collected from 42 men with clinical CHD and 44 healthy controls 40 -70 years of age. Pre-␤-HDL was assayed by crossed immunoelectrophoresis. Results: Cases had lower HDL-cholesterol (؊23%), total apolipoprotein A-I (؊26%), and pre-␤-HDL (؊55%; all P <0.001) concentrations; lower pre-␤-HDL:␣-HDL ratios (؊45%; P <0.001); and higher plasma triglycerides (20%; P <0.03) than the controls. On stepwise logistic regression, CHD was associated most strongly with pre-␤-HDL concentrations. On ROC analysis, pre-␤-

Pre-β-HDL formation relates to high-normal free thyroxine in type 2 diabetes mellitus

“…The pre-β-HDL levels reported here are closely comparable to those reported previously using different analytical methods [14,15,25], but higher absolute plasma pre-β-HDL levels have been demonstrated in other reports [12,26]. Although the Table 3 Univariate correlations of thyroid function parameters with plasma phospholipid transfer protein (PLTP) activity, total cholesterol, triglycerides, high density lipoprotein (HDL) cholesterol, apolipoprotein A-I (apoA-I), HDL cholesterol/apoA-I ratio, pre-β-HDL and pre-HDL formation in 72 subjects with Type 2 diabetes mellitus (T2DM) and 82 non-diabetic subjects.…”

“…By promoting cellular cholesterol efflux, pre-β-HDL particles play an important role in the reverse cholesterol transport pathway, whereby cholesterol is transported from peripheral cells back to the liver for biliary transport and excretion in the feces [8,[9][10][11]. Although not unequivocally reported [12], higher plasma pre-β-HDL concentrations are observed in subjects with cardiovascular disease [13,14]. Of further relevance, higher plasma pre-β-HDL levels associate with a greater cIMT, both in diabetic and non-diabetic subjects [15,16].…”

Molecular Biology of Atherosclerosis

“…Indeed, pre-␤ HDL levels in plasma can reflect multiple competing metabolic effects (e.g., initial generation of APOA1 and precursor particles, conversion to larger particles both after interaction with ABCA1 and ABCG1 and via the LCAT reaction, regeneration of pre-␤ HDL by CETP-and/or PLTPmediated speciation of triglyceride-enriched spherical HDL, interactions with hepatic and endothelial lipases, renal loss, and transfer into the interstitial fluid space). Perhaps not surprisingly, in other studies using different methods to directly measure plasma pre-␤ HDL, associations with CAD ranged from significantly positive to significantly negative (733,1221,1508). Therefore, in some settings, higher pre-␤ HDL plasma concentrations may manifest an indirect association with increased CAD risk even though pre-␤ HDL itself appears to be mechanistically protective.…”

The expression of ATP-binding cassette transporter A1 in Chinese overweight and obese patients

“…Total cholesterol, low-density lipoprotein (LDL) cholesterol, HDL cholesterol and triglyceride were determined on HITACH 7170 biochemical autoanalyzer. Plasma prebHDL level was determined by crossed immunoelectrophoresis as previously described by Hattori et al 18 The levels of adiponectin, resistin and leptin were measured by commercially available kits according to the instructions of the manufacturers. Plasma adiponectin was measured by enzyme-linked immunosorbent assay (ELISA; B-Bridge International, Phoenix, AZ, USA).…”