Association of CYP1A1 MspI polymorphism with oral cancer risk in Asian populations: a meta-analysis

Abstract

ABSTRACT. Numerous studies regarding the association between the CYP1A1 MspI polymorphism and oral cancer risk in Asian populations have shown controversial results. To get a more precise estimation of this relationship, we conducted a comprehensive meta-analysis. PubMed, the Cochrane Library, Elsevier Science Direct, Web of Knowledge, the Chinese National Knowledge Infrastructure, VIP, and Wan Fang Med Online were searched. Pooled odds ratios (ORs) with 95% confidence intervals (95%CIs) were calculated using fixed-effects or randomeffects models. Heterogeneity among studies was assessed using the Cochran Q test and I 2 statistics. Twelve articles including 1925 oral cancer patients and 2335 controls were ultimately included in the metaanalysis. Overall, the meta-analysis showed that the CYP1A1 MspI polymorphism was associated with oral cancer risk in Asians (m1/m1 vs m2/m2: OR = 0.46, 95%CI = 0.30-070, P OR = 0.000; m1/m1 vs m1/ m2+m2/m2: OR = 0.70, 95%CI = 0.51-0.98, P OR = 0.037; m1/m1+m1/ m2 vs m2/m2: OR = 0.48, 95%CI = 0.35-0.65, P OR = 0.000). Subgroup analyses showed that the control source (hospital-based or populationbased), the genotyping method [polymerase chain reaction (PCR) or PCR-restriction fragment length polymorphism], the country in which the study was conducted, and Hardy-Weinberg equilibrium (Yes or No) were positively related to the association. Sensitivity analysis suggested that the overall results showed no significant change in three genetic models when any one study was removed, and publication bias was undetected by the Egger test. The CYP1A1 MspI polymorphism may be associated with oral cancer risk in Asian populations.

The expression and significance of the <i>HOXA7</i> gene in oral squamous cell carcinoma

“…Briefly, segments of tissue of about 1-2 mm 3 were harvested and immediately stored in RNALater reagent (−80°C). The tissue was then transferred to lysis buffer containing proteolysis enzyme K and incubated at 55°C until complete digestion.…”

“…Oral squamous cell carcinoma (OSCC) accounts for more than 80% of all malignant oral and maxillofacial tumors. According to the latest data, mortality due to oral carcinoma accounts for 0.88% of all cancer deaths in China (2,3). In the United States, 2-4% of annually diagnosed malignancies are OSCC, resulting in 8,000 deaths each year (4).…”