Association of Diesel Exhaust Particle Exposure With Erythrocytes Deformation of Male Mice

Wardoyo, Arinto Yudi Ponco; Juswono, Unggul P.; Noor, Johan A. E.

doi:10.15666/aeer/1605_55835593

Cited by 5 publications

(5 citation statements)

References 36 publications

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“…This study used healthy male mice with the age of 2-3 months old (with 23-30 grams of BW (body weight)). The mice were treated humanely and feed with food and water ad libitum and kept in controlled animal cages (temperature: 24 -27 o C, relative humidity: 75 -78%) with 12 h dark/ light cycle [12]. All of them (n = 21) were acclimatized in an experimental chamber (20 x 20 x 30 cm 3 ) for three consecutive days before being divided randomly into two treatment groups (Group-A and Group-B) and one control group (Group-C).…”

Section: Materials and Methods 21 Experimental Animalsmentioning

confidence: 99%

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The Impact of Exposure to Aerosol Mosquitoes Repellent Related to Free Radicals on the Organs of Male Mice

Juswono¹

2020

GEOMATE

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From year to year, human activities using chemical substances lead to pollute ambient air and affect human health. This pollutant can pass through the respiratory system and disrupts the organ function, including lung, liver, blood, and kidney. It can be identified by the deformation found in the structure and function change of the related cells in these organs and the existence of free radical. On the other hand, each substance has a different composition and health effect. The possibility of the free radical type that is formed is also different and related to the substance. According to this background, it a need to determine the presence of free radicals in the mice's organs exposed to one push aerosol mosquito repellents as the substance. This study aimed to investigate the free radicals on the mice's organs due to the exposure to aerosol mosquito repellent and its relation with the organ damage of cell deformation. This study used male mice as the experimental animals that were divided into control and treatment groups. The study included two types of repellents, Type-A (25% of aerosol concentration) and Type-B (21.3% of aerosol concentration). Free radicals were detected using an electron spin resonance device. The results showed that the cell deformation due to the exposure of one push aerosol was different for each organ. The deformation levels were about 36% to 74% that depended on the aerosol concentration. The exposed aerosol generated anion superoxide (O2-) and singlet oxygen radicals (1O2) as the free radicals found in the observed organs.

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Section: Materials and Methods 21 Experimental Animalsmentioning

confidence: 99%

“…After that, these samples were colored using Giemsa and buffer pro-Giemsa solution. All resulted samples were observed under a microscope (400x of magnification) to investigate the level of cell physical damages (blind counting method) [12][13][14].…”

Section: Organs Preparationmentioning

confidence: 99%

The Impact of Exposure to Aerosol Mosquitoes Repellent Related to Free Radicals on the Organs of Male Mice

Juswono¹

2020

GEOMATE

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“…1) [20]. After exposures, the mice were sacrificed to observe organ samples using the cervical vertebra dislocation method [21].…”

Section: Transfluthrin Exposurementioning

confidence: 99%

Correlation Between Exposure to Transfluthrin and the Change in Dielectric Properties and Deformed Cells of Mice

Juswono¹,

Wardoyo²,

Widodo³

et al. 2020

Pol. J. Environ. Stud.

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Transflutrhin is a single isomer compound that is contained in mosquito repellent. The volatility of this chemical compound made it suited for applications that evaporate this active ingredient like space spray using one push mosquito repellent. As an aerosol, transfluthrin can trigger oxidative stress that causes cell damage. Cell damage can be identified microscopically or by analyzing the electrical characteristics of the cell. Cell damage due to the content of chemical compounds in the pollutants that enter the body will affect the electrical properties of the cell itself. One of the bio-electric variables in organ tissues that can be analyzed and used to determine the level of tissue damage is a change in the dielectric constant value of an organ. The measurement of the dielectric constant is done by using two conductor plates in which the dielectric material is inserted in the middle of the plates, and the capacitance value will be measured. The results show that a higher level of damage to the organ caused a lower dielectric constant value. This decrease was caused by the changes in the polarization properties of the damaged tissue. Factors that affected the polarization of material were large electric dipole moments and large numbers of healthy and damaged cells. Organ damage was determined based on the decrease in the value of dielectric constant. Histological studies revealed severe cell damages evidenced by the deformed cell compared to the unexposed mice. Type-A mosquito repellents with transfluthrin active ingredients of 25% caused 74% at lung cell damage, 71% of blood damage, 47% of kidney cell damage, and 40% of liver cell damage, with dielectric organ constants 0.65x10 5 to 2.62x10 5. In the mice exposed to type-B mosquito repellent (21.3% of transfluthrin), the damage levels were 72%, 72%, 49%, and 36%, respectively, for lung, blood, kidney, and liver cells. The dielectric constants were 0.91x10 5 to 2.62x10 5. Our studies indicate that mosquito repellent aerosol does initiate physical damage

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“…Further investigation confirmed more impacts of PM 0.1 on human health than other bigger particulate matters [15][16]. Specific to PM 0.1 and PM 2.5 , some studies examined the deformed erythrocytes and alveolar cells due to motor vehicle emissions exposure, where the deformation levels are dominated in alveolar cells [10,[17][18]. Moreover, an in vitro study using mice confirmed that exposure to PM 2.5 exerts a discernible effect on promoting hepatic fibrogenesis under the normal chow or high-fat diet [19].…”

Section: Introductionmentioning

confidence: 98%

A Study of Erythrocyte Deformation Level Related to Biomass Burning Emission Exposures Using Artificial Neural Networks

Hadi¹,

Wardoyo²,

Juswono³

et al. 2022

Pol. J. Environ. Stud.

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Emissions from burning biomass have become a problem in Indonesia. As found on the Indonesian island of Lombok, agricultural waste is burned for traditional industrial activities. On the other hand, biomass burning emissions contain many PMs (particulates) in different size distributions recognized to have a significant correlation to health impact. This study is conducted to predict the impact of the PM exposure on blood using a ANN (artificial neural network) model as well as a histological examination. The relationship between both methods is determined to estimate the impact of biomass burning emissions on the blood. This study used male mice as the experimental animals exposed to PM emissions (PM 0.1 , PM 2.5 , and PM 10 ) produced from the burning of various biomass (rice straw, rice husks, corn cobs, corn stalks, and tobacco) taken from Lombok Island. The sample exposure was conducted in a chamber for 100 s for ten sequence days. The blood samples were observed using a microscope with the 400 x magnification. The cell deformation was examined histologically by calculating the normal and abnormal cells. The percentage of the erythrocyte deformation was assessed using a fixed back and forth propagation ANN. The result shows that the biomass burning PM emissions have a significant impact on the erythrocyte deformation depending on the type of biomass and the particulate matter emissions. The ANN model confirms the erythrocyte deformation data obtained by the histological examination method.

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Association of Diesel Exhaust Particle Exposure With Erythrocytes Deformation of Male Mice

Cited by 5 publications

References 36 publications

The Impact of Exposure to Aerosol Mosquitoes Repellent Related to Free Radicals on the Organs of Male Mice

The Impact of Exposure to Aerosol Mosquitoes Repellent Related to Free Radicals on the Organs of Male Mice

Correlation Between Exposure to Transfluthrin and the Change in Dielectric Properties and Deformed Cells of Mice

A Study of Erythrocyte Deformation Level Related to Biomass Burning Emission Exposures Using Artificial Neural Networks

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