Association of Duffy Blood Group Gene Polymorphisms with IL8 Gene in Chronic Periodontitis

Abstract

The antigens of the Duffy blood group system (DARC) act as a receptor for the interleukin IL-8. IL-8 plays an important role in the pathogenesis of chronic periodontitis due to its chemotactic properties on neutrophils. The aim of this study was to investigate a possible association of Duffy blood group gene polymorphisms with the -353T>A, -845T>C and -738T>A SNPs of the IL8 gene in chronic periodontitis. One hundred and twenty-four individuals with chronic periodontitis and 187 controls were enrolled. DNA was extracted using the salting-out method. The Duffy genotypes and IL8 gene promoter polymorphisms were investigated by PCR-RFLP. Statistical analyses were conducted using the Chi square test with Yates correction or Fisher's Exact Test, and the possibility of associations were evaluated by odds ratio with a 95% confidence interval. When analyzed separately, for the Duffy blood group system, differences in the genotype and allele frequencies were not observed between all the groups analyzed; and, in nonsmokers, the -845C allele (3.6% vs. 0.4%), -845TC genotype (7.3% vs. 0.7%) and the CTA haplotype (3.6% vs. 0.4%) were positively associated with chronic periodontitis. For the first time to our knowledge, the polymorphisms of erythroid DARC plus IL8 -353T>A SNPs were associated with chronic periodontitis in Brazilian individuals. In Afro-Brazilians patients, the FY*02N.01 with IL8 -353A SNP was associated with protection to chronic periodontitis.

Quantitative Assessment of the Associations Between Interleukin‐8 Polymorphisms and Periodontitis Susceptibility

“…1). Basic characteristics and the scores of study quality assessment of selected articles 15‐26 are all listed in Table 1. Overall, all included studies were of moderate‐to‐high quality, with a mean quality score of 27.4/40 (range: 23 to 30).…”

“…Overall, all included studies were of moderate‐to‐high quality, with a mean quality score of 27.4/40 (range: 23 to 30). Eight studies enrolled non‐smokers, providing an analysis inhibiting the influence of the smoking risk factor 16‐18,21,23‐26 . Six eligible studies mentioned quality control of the genetic analyses, such as mask genotyping, random replication, and validated genotyping accuracy 16,19,20,22,25,26 .…”

“…Eleven studies evaluated the effect of IL‐8 −251A/T polymorphism on periodontitis risk 15‐22,24‐26 . Overall, considerable between‐study heterogeneity was observed in all five genetic models (Table 2).…”

“…Three studies investigated the potential associations of IL‐8 −845T/C polymorphism with either CP or AgP 17,19,23 . Between‐study heterogeneity was not evident (Table 2).…”

“…Nevertheless, subsequent research by Andia et al suggested that this polymorphism may play a protective role in periodontitis risk in non‐smoking Brazilian populations 16 . Until now, results of different studies remain inconsistent rather than conclusive, 17‐26 which was likely due to small sample sizes and low statistical power. To better clarify the associations between polymorphisms in IL‐8 gene and periodontitis susceptibility, this review reports the performance of a quantitative meta‐analysis by collecting and analyzing the published data.…”