Association of dupA, iceA, homB Genes of Helicobacter pylori with Gastritis, Peptic Ulcer Disease and Gastric Cancer

Abstract

Objective: To determine the association of dupA, iceA, homB genes of Helicobacter pylori with gastro-duodenal diseases such as gastritis, peptic ulcer disease PUD and gastric cancer.
 Materials and Methods: This cross-sectional analytical study was conducted at Gastroenterology Department, Shaikh Zayed Hospital Lahore. Patients with gastro-duodenal diseases and positive H. pylori were included. Gastric biopsies were taken from fundus, body and antrum. H. pylori DNA were removed utilizing Gentra DNA extraction Kit (Life Technologies, USA) as per the technique and Qualitative PCR for the recognition of H. Pylori DNA. The PCR primers sets were designed for the specific detection of dupA, iceA and homB genes of H. pylori. All the data was recorded in proforma and analyzed by SPSS version 20.
 Results: Mean age of the cases was 41.22+8.04 years. Males were more affected 118(60.2%). HomB was the most common 76(38.8%) followed by dupA and iceA 28.6% and 24.5% respectively. Peptic ulcer disease and gastritis were higher among patients having dup A and iceA positive strains as compared to homB gene patients, while gastric cancer was significantly higher among HomB gene infected patients, p-values were quite significant.
 Conclusion: It was concluded that homB gene was most frequent in H. pylori infected population. Peptic ulcer disease and gastritis are markedly associated with dupA and iceA genes, while homB gene infected patients are at high risk of gastric cancer.

Helicobacter pylori Infection and Circulating Ghrelin Levels- A Review

“…Because cells that produce hunger hormone are localized in stomach, it stands to reason that chronic gastritis and atrophy would impede hunger hormone manufacture , acylation, &/or emission , affecting hunger, weight, and BMI. Some of the discrepancies in the literature about the effect of H. pylori infection on circulating ghrelin levels could be because of difference among people and civilization, illness severity (for ex, presence or absence of atrophy), and Helicobacter pylori strain dissimilarity [68]. This is further exacerbated by the fact that ghrelin is measured using a variety of immunoassays and that acylated ghrelin is not stable and deteriorate fast to unacylated ghrelin [42].…”