Association of follicular fluid volume with membrane stretchability of human metaphase <scp>II</scp> oocytes following the gonadotropin‐releasing hormone agonist protocol during intracytoplasmic sperm injection

Inoue, Taketo; Taguchi, Sayumi; Hirao, Kayoko; Tsujimoto, Yoshiko; Yamamoto, Shuji; Uemura, Mikiko; Miyazaki, Kazunori; Yamashita, Yoshiki

doi:10.1002/rmb2.12102

Cited by 4 publications

(1 citation statement)

References 30 publications

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“…Controlled ovarian stimulation was performed using a long, short, or gonadotropin‐releasing hormone (GnRH) antagonist protocol depending on the patient as described previously. 16 , 17 , 18 A GnRH analogue acetate (Fuji Pharma Co., Tokyo, Japan), human menopausal gonadotropin (ASKA Pharmaceutical Co., Tokyo, Japan), and a GnRH antagonist (Cetrotide; Merck KGaA, Darmstadt, Germany) were administered as dictated in these established protocols. When at least 2 follicles reached 18–20 mm in diameter (as determined by transvaginal ultrasonography), 5000 IU of hCG (Fuji Pharma Co.) was administered.…”

Section: Methodsmentioning

confidence: 99%

The migration speed of nucleolar precursor bodies in pronuclei affects in vitro fertilization‐derived human embryo ploidy status and live birth

Inoue

Taguchi²,

Uemura

et al. 2023

Reprod Medicine & Biology

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Purpose To study the relationship between clinical outcomes after assisted reproduction and the migration speed of nucleolus precursor bodies (NPBs) in male and female pronuclei (mPN; fPN). Methods NPB migration speed, embryo ploidy status, and live birth (LB) were retrospectively analyzed in IVF‐derived zygotes. The central coordinates of the mPN, fPN, and NPBs were noted at multiple timepoints. The migration distance of NPBs between two sequential images was measured to calculate NPB migration speed. Results The NPB migration speeds in mPN and fPN were significantly faster in euploid zygotes than in aneuploid zygotes. In multivariate logistic analysis, NPB migration speed in mPN and the female age were associated with euploidy. The NPB migration speeds in mPN and fPN were also significantly faster in zygotes that led to LB than in zygotes that led to no pregnancy. In a receiver operating characteristic curve analysis of LB by NPB migration speed in mPN, the cut‐off value was 3.74 μm/h (AUC: 0.825, 95%CI: 0.688–0.963). When the zygotes were categorized by this cut‐off value, there were significantly more LBs in zygotes with migration speed ≥ the cut‐off (78.9% vs. 21.1%). Conclusions Zygotes with quickly migrating NPBs demonstrated the developmental potential to become a baby.

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Section: Methodsmentioning

confidence: 99%

The migration speed of nucleolar precursor bodies in pronuclei affects in vitro fertilization‐derived human embryo ploidy status and live birth

Inoue

Taguchi²,

Uemura

et al. 2023

Reprod Medicine & Biology

Self Cite

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The relationship between controlled ovarian stimulation protocol, meiotic spindle visibility, position of the meiotic spindle relative to the polar body in the human oocyte, and clinical outcomes following ICSI

Inoue,

Matsuo,

Taguchi

et al. 2024

Reprod Medicine & Biology

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PurposeTo investigate the effects of different controlled ovarian stimulation (COS) protocols, including the progestin‐primed ovarian stimulation (PPOS), long, short, and the gonadotropin‐releasing hormone antagonist protocols, on meiotic spindle visibility and position within the oocyte and clinical outcomes following ICSI.MethodsBefore ICSI, spindle position (θ) just below the polar body (PB) was defined as 0° and categorized as follows: θ = 0°, 0° < θ ≤ 30°, 30° < θ ≤ 60°, 60° < θ ≤ 90°, 90° < θ ≤ 180°, between the PB and the oolemma, and nonvisible. The clinical outcomes after ICSI were retrospectively analyzed.ResultsThe normal fertilization rate was significantly higher in oocytes with visible spindles than in oocytes with nonvisible spindles after each COS protocol, but did not differ based on spindle positioning (0° ≤ θ ≤ 180°). The rates of pregnancy, live birth/ongoing pregnancy, and miscarriage did not differ based on spindle visibility or positioning. In multinominal logistic regression analysis, female age was associated with spindle position, and the odds of a spindle located at 30° < θ ≤ 60°, at 60° < θ ≤ 90°, or at 90° < θ ≤ 180° were increased relative to θ = 0° in older women (odds ratio; 1.020, 1.030, and 1.060, respectively; p < 0.05).ConclusionMeiotic spindle positioning in the oocyte does not affect normal fertilization, blastulation, pregnancy, live birth/ongoing pregnancy, and miscarriage after ICSI, independent of the COS protocol used.

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Human metaphase II oocytes with narrow perivitelline space have poor fertilization, developmental, and pregnancy potentials

Shioya,

Okabe-Kinoshita,

Kobayashi

et al. 2024

J Assist Reprod Genet

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Association of follicular fluid volume with membrane stretchability of human metaphase II oocytes following the gonadotropin‐releasing hormone agonist protocol during intracytoplasmic sperm injection

Cited by 4 publications

References 30 publications

The migration speed of nucleolar precursor bodies in pronuclei affects in vitro fertilization‐derived human embryo ploidy status and live birth

The migration speed of nucleolar precursor bodies in pronuclei affects in vitro fertilization‐derived human embryo ploidy status and live birth

The relationship between controlled ovarian stimulation protocol, meiotic spindle visibility, position of the meiotic spindle relative to the polar body in the human oocyte, and clinical outcomes following ICSI

Human metaphase II oocytes with narrow perivitelline space have poor fertilization, developmental, and pregnancy potentials

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