Association of Gene Polymorphisms of Antioxidants with Reproductive Losses

The Role of rs713041 Glutathione Peroxidase 4 (GPX4) Single Nucleotide Polymorphism on Disease Susceptibility in Humans: A Systematic Review and Meta-Analysis

“…The selected studies reported data related to GPX4 (rs713041) SNP linked to nineteen diseases; these included breast cancer [ 16 , 17 ], colorectal cancer [ 35 , 36 ], prostate cancer [ 20 , 37 ], laryngeal cancer [ 19 ], lung cancer [ 19 ], pre-eclampsia [ 38 , 39 ], hypertension [ 40 ], ischemic stroke [ 41 ], endometriosis [ 22 ], recurrent miscarriage [ 42 ], pregnancy loss [ 43 ], Graves’ disease [ 23 ], Hashimoto disease [ 23 ], acute pancreatitis [ 44 ], Alzheimer’s disease [ 24 ], depression [ 25 ], multiple sclerosis [ 26 ], type 2 diabetes mellitus [ 45 ] and Kashin-Beck disease [ 46 ]. Regarding demographics, from the twenty-one reports, one study was performed on Arabs [ 45 ], two reports on Russians [ 42 , 43 ], one report on Caucasian Americans [ 36 ], one report on South Americans [ 24 ], five reports on Asians, Han Chinese population [ 22 , 23 , 38 , 39 , 46 ], and the other eleven reports on Europeans [ 16 , 17 , 19 , 20 , 25 , 26 , 35 , 37 , 40 , 41 , 44 ]. Genotyping was carried out by different methods, two reports using real-time PCR [ 19 ], one KASPar [ 35 ...…”

“…Regarding demographics, from the twenty-one reports, one study was performed on Arabs [ 45 ], two reports on Russians [ 42 , 43 ], one report on Caucasian Americans [ 36 ], one report on South Americans [ 24 ], five reports on Asians, Han Chinese population [ 22 , 23 , 38 , 39 , 46 ], and the other eleven reports on Europeans [ 16 , 17 , 19 , 20 , 25 , 26 , 35 , 37 , 40 , 41 , 44 ]. Genotyping was carried out by different methods, two reports using real-time PCR [ 19 ], one KASPar [ 35 ], two reports MassArray [ 23 , 37 ], four reports PCR-RFPL [ 42 , 43 , 44 , 46 ] and the remaining twelve reports using TaqMan assay [ 16 , 17 , 20 , 22 , 24 , 25 , 26 , 36 , 38 , 39 , 40 , 41 , 45 ]. The characteristics, genotyping methods, sample size and genotype frequency of the twenty one reports are presented in Table 1 .…”

“…In an analysis of diseases related to the female reproductive system, 513 cases and 443 controls were included in the initial meta-analysis. Three studies were used: one report evaluated endometriosis [ 22 ], one miscarriage [ 42 ] and one pregnancy loss [ 43 ]. Regarding demographics and genotyping methods, the endometriosis report was performed on the Han Chinese population using TaqMan assay [ 22 ] and the miscarriage and pregnancy loss on Russian women using PCR-RFLP [ 42 , 43 ].…”

“…Three studies were used: one report evaluated endometriosis [ 22 ], one miscarriage [ 42 ] and one pregnancy loss [ 43 ]. Regarding demographics and genotyping methods, the endometriosis report was performed on the Han Chinese population using TaqMan assay [ 22 ] and the miscarriage and pregnancy loss on Russian women using PCR-RFLP [ 42 , 43 ]. Figure 4 shows the meta-analysis results for reproduction-related diseases and the association with GPX4 (rs713041) SNP.…”

“… Forest plots of odds ratios with 95% confidence intervals of the association between GPX4 (rs713041) SNP and risk of reproduction-related diseases. ( A )—Additive model (TT vs. CC) [ 22 , 42 , 43 ], ( B )—Dominant model (CT+TT vs. CC) [ 22 , 42 , 43 ] and ( C )—Recessive model (TT vs. CC+CT) [ 22 , 42 , 43 ]. …”