Association of hepatitis B virus subgenotypes and basal core promoter/precore region variants with the clinical features of patients with acute hepatitis

Hayashi, Kazuhiko; Katano, Yoshiaki; Takeda, Yasushi; Honda, Takashi; Ishigami, Masatoshi; Itoh, Akihiro; Hirooka, Yoshiki; Nakano, Isao; Yoshioka, Kentaro; Toyoda, Hidenori; Kumada, Takashi; Goto, Hidemi

doi:10.1007/s00535-008-2197-2

Cited by 28 publications

(21 citation statements)

References 34 publications

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“…These findings suggest that subgenotype B4 and BCP/PC variants have an association with progression to fulminant hepatic failure. Therefore, investigation of BCP/PC variants in subgenotype B4 will be useful for predicting fulminant hepatic failure and developing treatment protocols, as has been previously reported for subgenotype C2/Ce [14] .…”

Section: Discussionmentioning

confidence: 99%

“…The HBV-DNA quantitative viremia load was determined using real-time PCR [13] . Nested polymerase chain reaction (PCR) analysis and direct sequencing of the preS, polymerase, and PC/core regions were performed as reported previously [14] . In brief, each 50-l PCR reaction contained 100 n M each primer, 1 ng template DNA, 5 l GeneAmp 10 !…”

Section: Dna Amplification and Sequencingmentioning

confidence: 99%

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Prevalence of Hepatitis B Virus Subgenotypes and Basal Core Promoter, Precore Variants in Patients with Acute Hepatitis B in Central Vietnam

et al. 2009

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Objective: Hepatitis B virus (HBV) has been classified into 8 genotypes that have different geographic distributions. The clinical outcomes of acute hepatitis are dependent on genotype. The aim of this study was to investigate the distribution of HBV subgenotypes and basal core promoter (BCP)/precore (PC) regions in acute hepatitis patients in Central Vietnam to clarify the distributions and the clinical and virological differences. Methods: 27 patients with acute hepatitis B were studied. HBV subgenotypes and BCP/PC variants were determined by direct sequencing of the preS, BCP/PC regions, respectively. Results: HBV subgenotypes B4/Ba (n = 22) and C1/Cs (n = 5) were detected. Of the 27 patients, 3 developed fulminant hepatic failure, and all were infected with B4/Ba. Three patients had a BCP mutation, and 10 patients had a PC mutation in subgenotype B4/Ba. Three patients with C1/Cs had a BCP mutation. Two of 3 patients who progressed to fulminant hepatic failure had T1762, A1764, and A1896 simultaneously. None of the patients with acute, self-limited hepatitis carried these triple mutations. Conclusion: The prevalent HBV subgenotypes in patients with acute hepatitis B in Central Vietnam were B4/Ba and C1/Cs. BCP/PC variants have an association with the development of fulminant hepatic failure in subgenotype B4/Ba.

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Section: Discussionmentioning

confidence: 99%

Section: Dna Amplification and Sequencingmentioning

confidence: 99%

Prevalence of Hepatitis B Virus Subgenotypes and Basal Core Promoter, Precore Variants in Patients with Acute Hepatitis B in Central Vietnam

et al. 2009

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“…Therefore, whether high replicative strains are associated with FH still remained controversial. Regarding the genotypes involved in FH, Liu et al [36] did not find that HBV genotypes differed between FH and subfulminant exacerbations in chronic HB patients, while Hayashi et al [37] concluded that basal core promoter/precore variants in subgenotype C2 could be associated with progression to FH.…”

Section: Studies On Isolates From Fulminant Hepatitis B Patientsmentioning

confidence: 97%

Biological features of hepatitis B virus isolates from patients based on full‐length genomic analysis

Wen

Wang

2008

Reviews in Medical Virology

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The mechanisms for HBV persistence and the pathogenesis of chronic HB have been shown mainly due to defects in host immune responses. However, HBV isolates with different biological features may also contribute to different clinical outcomes and epidemiological implications in viral hepatitis B (HB). This review presents interesting biological features of HBV isolates based on the structural and functional analysis of full-length HBV isolates from various patients. Among isolates from children after failure of HB vaccination, 129L mutant at the 'a' determinant was found with normal binding efficiency to anti-HBs, but with reduced immunogenicity, which could initiate persistent HBV infections. Isolates from fulminant hepatitis (FH) B patients were not all highly replicative, but differences in capacities of anti-HBs induction could be involved in the pathogenesis of FH. The high replicative competency of isolates from hepatocellular carcinoma (HCC) patients could result in enhanced immune-mediated cytopathic effects against HBV viral proteins, and increased transactivating activity by the X protein. The mechanism of a double-spliced variant in enhancing replication of the wild-type virus is presented. The importance of integrating structural and functional analysis to reveal biological features of HBV isolates in viral pathogenesis is discussed.

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“…It is well recognized that a low level of HBV persists in the liver and peripheral blood mononuclear cells in patients who have recovered from acute HBV infection [11,12]; subsequent immunosuppressive or cytotoxic chemotherapy may change the immune functions that control HBV replication and thus trigger HBV reactivation in these patients. The possible mechanisms involved have been discussed speculatively, but the precise clinical characteristics and virological features remain unclear, including the prevalence of HBV genotypes and of mutations known to be associated with HBV pathogenesis, such as A1762T, G1764A in the basal core promoter (BCP) region, G1896A in the precore (PC) region, and those in the S gene region [9,10,[13][14][15][16]. We studied the clinical and virological characteristics associated with HBV reactivation during or after immunosuppressive or cytotoxic chemotherapy in HBsAg-negative patients to clarify the pathogenesis of HBV reactivation.…”

Section: Introductionmentioning

confidence: 99%

Clinical characteristics and molecular analysis of hepatitis B virus reactivation in hepatitis B surface antigen-negative patients during or after immunosuppressive or cytotoxic chemotherapy

et al. 2016

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Association of hepatitis B virus subgenotypes and basal core promoter/precore region variants with the clinical features of patients with acute hepatitis

Abstract: BCP/PC variants would be associated with progression to fulminant hepatitis in subgenotype C2. Knowledge of HBV subgenotypes and BCP/PC variants is useful for developing strategies to treat acute hepatitis B patients.

Cited by 28 publications

References 34 publications

Prevalence of Hepatitis B Virus Subgenotypes and Basal Core Promoter, Precore Variants in Patients with Acute Hepatitis B in Central Vietnam

Prevalence of Hepatitis B Virus Subgenotypes and Basal Core Promoter, Precore Variants in Patients with Acute Hepatitis B in Central Vietnam

Biological features of hepatitis B virus isolates from patients based on full‐length genomic analysis

Clinical characteristics and molecular analysis of hepatitis B virus reactivation in hepatitis B surface antigen-negative patients during or after immunosuppressive or cytotoxic chemotherapy

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