Association  of HindIII-polymorphism  in  kappa-casein  gene  with   milk,  fat  and  protein  yield  in  holstein  cattle*

Miluchová, Martina; Gábor, Michal; Candrák, Juraj; Trakovická, Anna; Candráková, Kristína

doi:10.18388/abp.2017_2313

Cited by 29 publications

(15 citation statements)

References 19 publications

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“…Comparative amino acid sequence analysis revealed that CN protein harbor higher amino acid variations in river buffalo (Mediterranean and Murrah) as compared to the swamp buffalo. The results of the present study are in line with previous studies (Masina et al, 2007;Azevedo et al, 2008;Massella et al, 2017;Rangel et al, 2017;Miluchova et al, 2018;Fan et al, 2020;de Oliveira et al, 2021) which reported the potential association of genetic variants in CSN genes with lactation performance, milk composition, and attributes of milk products. Thus, casein gene-based markers are important candidates for the selective breeding of buffalo to improve the quantity and quality of milk (de Oliveira et al, 2021).…”

Section: Discussionsupporting

confidence: 93%

Comparative Genomics, Evolutionary and Gene Regulatory Regions Analysis of Casein Gene Family in Bubalus bubalis

Rehman

Tong-qing

et al. 2021

Front. Genet.

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Buffalo is a luxurious genetic resource with multiple utilities (as a dairy, draft, and meat animal) and economic significance in the tropical and subtropical regions of the globe. The excellent potential to survive and perform on marginal resources makes buffalo an important source for nutritious products, particularly milk and meat. This study was aimed to investigate the evolutionary relationship, physiochemical properties, and comparative genomic analysis of the casein gene family (CSN1S1, CSN2, CSN1S2, and CSN3) in river and swamp buffalo. Phylogenetic, gene structure, motif, and conserved domain analysis revealed the evolutionarily conserved nature of the casein genes in buffalo and other closely related species. Results indicated that casein proteins were unstable, hydrophilic, and thermostable, although αs1-CN, β-CN, and κ-CN exhibited acidic properties except for αs2-CN, which behaved slightly basic. Comparative analysis of amino acid sequences revealed greater variation in the river buffalo breeds than the swamp buffalo indicating the possible role of these variations in the regulation of milk traits in buffalo. Furthermore, we identified lower transcription activators STATs and higher repressor site YY1 distribution in swamp buffalo, revealing its association with lower expression of casein genes that might subsequently affect milk production. The role of the main motifs in controlling the expression of casein genes necessitates the need for functional studies to evaluate the effect of these elements on the regulation of casein gene function in buffalo.

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Section: Discussionsupporting

confidence: 93%

Comparative Genomics, Evolutionary and Gene Regulatory Regions Analysis of Casein Gene Family in Bubalus bubalis

Rehman

Tong-qing

et al. 2021

Front. Genet.

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“…Однако большинство авторов по итогам своих исследований находият, что молоко от коров с превалированием аллеля В по гену CSN3 характеризуется высоким содержанием жира, белка, сухих веществ и лучшей сыропригодностью [12][13][14]18]. Следовательно, в рамках рациональной переработки целесообразно использовать молоко для производства сыров и продуктов молочнокислого брожения.…”

Section: Discussionunclassified

“…В качестве одного из таких маркеров рассматривается ген ϰ-казеина (CSN3), локализованный на 6-ой хромосоме генома Bos taurus [11,12]. Из 13 охарактеризованных вариантов гена ϰ-казеина крупного рогатого скота часто встречаемыми у большинства пород молочного направления являются аллели А и В CSN3, кодирующие соответствующие структурные элементы белков, различающиеся двумя аминокислотными заменами в 136 и 148 положениях полипептидной цепи [13,14].…”

Section: Introductionunclassified

“…В научной литературе аллели А и В гена CSN3 признаны взаимосвязанными с молочной продуктивностью, а также составом и технологическими свойствами молока [11,12,[14][15][16]. Молоко от коров с генотипами AB и BB имеет диаметр products.…”

Section: Introductionunclassified

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New determination method for the ratio of the relative proportions of ϰ-casein alleles in milk powder

Gilmanov

Semipyatny

Бигаева

et al. 2020

Food Processing: Techniques and Technology

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Introduction. High-quality dairy products depend on the properties of raw materials. Milk is the basic raw material for all dairy products. Its quality is subject to requirements of biological safety, and its physicochemical and technological properties comply with strict indicators. ϰ-casein gene is a basic milk protein. Its allelic variants affect milk productivity, as well as its composition and technological properties, e.g. cheeseability and heat resistance. The research objective was to develop a new method for determining the ratio of the relative proportions of selection-significant kappa-casein gene alleles in bulk cow’s milk powder by means of molecular genetics and bioinformation system. The method can help to test raw materials for further processing rationality. Study objects and methods. The research included a genetic analysis of samples of bulk whole milk powder, bulk powdered skimmed milk, and bulk raw milk. The developed method involved DNA extraction, combined PCR-RFLP technique, electrophoretic results, and analysis detection of information data using new mathematical algorithms and software. Results and discussion. The ratio of the relative proportions of the kappa-casein gene alleles in milk powder was determined using primer sets JK5 and JK3, as well as restriction endonuclease HinfI for PCR-RFLP analysis. The experiment showed satisfactory reproducibility and interpretation of the obtained data. The program provided a ratio calculation of the kappa-casein gene alleles relative proportions in the studied milk powder samples, expressed as a percentage of the A allele proportion with additional indication of the absolute and relative errors. It also placed the information block of the generated numerical indicators into the percentage scale. Conclusion. The developed methodology was implemented by a set of laboratory procedures. The new specialized program “Calculation of the relative proportions ratio of ϰ-casein alleles in bulk milk” is on open access. It provides a correct and prompt data interpretation, generated during the analysis of dry milk raw materials of bulk origin.

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“…Mohammadi et al (2013) reported that the observed heterozygotes of κ-CN gene were extremely lower than the expected one in Iranian Holstein cattle with H o value of (0.18) and H e value of (0.31). Additionally, Miluchová et al (2018) inferred that the Holstein cattle breed exhibit a high value of homozygosity with H o value of (0.2762) and H e value of (0.7222).…”

Section: Heterozygosity and Inbreeding Coefficient (F Is ) Of κ-Cn Genementioning

confidence: 99%

Polymorphism, Allelic and Genotypic Frequencies of κ-Casein and β-LG genes in Egyptian Buffaloes

Al-Shawa¹,

El-Zarei²,

Ghazy³

et al. 2020

JAS

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With a view to detecting the genotypes of both κ-CN and β-LG genes in native populations of Egyptian buffalo using PCR-RFLP technique, 80 randomly, individuals were selected from five geographical locations of some Egyptian provinces. Also, to estimate the population genetic parameters such as, allelic and genotypic frequencies, heterozygosity, and inbreeding coefficient (F IS ) of these studied genes. For genotyping, 453 bp PCR product of κ-CN was digested with AcuI and HpyCH4IV (Isoschizomer for MaeII) restriction enzymes while the 247 bp PCR product of β-LG was digested with HaeIII restriction enzyme. PCR-RFLP results discovered polymorphism at the level of κ-CN gene in all studied Egyptian buffaloes with two distinct alleles "A" and "B". PCR-RFLP analysis for κ-CN gene using both restriction enzymes successfully detected that polymorphic status of the studied populations. We recommended using AcuI enzyme which was more capable for differentiating between homozygous (17%) and heterozygous (83%) individuals than HpyCH4IV enzyme which defined only 4% of homozygous individuals and the remaining was heterozygous (96%) individuals. Existence of heterozygosity excess in all studied populations referred to higher degree of genetic variability between individuals within these populations. On contrary, results of PCR-RFLP at the level of β-LG gene revealed a monomorphic pattern of Egyptian buffaloes and genotyped as "AA" animals which signified that PCR-RFLP assay with HaeIII enzyme for β-LG gene failed to discover any evidence of polymorphism in Egyptian buffalo under the circumstances of this study or all studied animals possess only one allele.

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Association of HindIII-polymorphism in kappa-casein gene with milk, fat and protein yield in holstein cattle*

Cited by 29 publications

References 19 publications

Comparative Genomics, Evolutionary and Gene Regulatory Regions Analysis of Casein Gene Family in Bubalus bubalis

Comparative Genomics, Evolutionary and Gene Regulatory Regions Analysis of Casein Gene Family in Bubalus bubalis

New determination method for the ratio of the relative proportions of ϰ-casein alleles in milk powder

Polymorphism, Allelic and Genotypic Frequencies of κ-Casein and β-LG genes in Egyptian Buffaloes

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