Association of BoLA‐DRB3 alleles identified by a sequence‐based typing method with mastitis pathogens in Japanese Holstein cows

Abstract

The association of the polymorphism of bovine leukocyte antigen (BoLA-DRB3) genes identified by the polymerase chain reaction sequence-based typing (PCR-SBT) method with resistance and susceptibility to mastitis caused by pathogenic bacteria was investigated. Blood samples for DNA extraction were collected from 194 Holstein cows (41 healthy cows and 153 mastitis cows including 24 mixed-infection cows infected with 2 or 3 species of pathogens) from 5 districts of Chiba prefecture, Japan. Sixteen BoLA-DRB3 alleles were detected. The 4 main alleles of DRB3*0101, *1501, *1201, and *1101 constituted 56.8% of the total number of alleles detected. Mastitis cows were divided into 2 groups: group 1 with single-infection cows and group 2 with all mastitis cows including 24 mixed-infection cows. The differences in the frequencies of BoLA-DRB3 alleles and the number of cows homozygous or heterozygous for each BoLA-DRB3 allele between healthy cows and the 2 groups of mastitis cows were evaluated. Furthermore, similar comparisons were performed between healthy cows and the 2 groups of mastitis cows for each mastitis pathogen. It was considered that the 4 alleles, namely, DRB3*0101, *1501, *1201, and *1101 had specific resistance and susceptibility to 4 different mastitis pathogens. Thus, DRB3*0101 might be associated with susceptibility to coagulase-negative Staphylococci and Escherichia coli, and DRB3*1501 might be associated with susceptibility to Escherichia coli. However, DRB3*1101 might be associated with resistance to Streptococci and coagulase-negative Staphylococci, and DRB3*1201, with resistance to Streptococci, Escherichia coli, and Staphylococcus aureus.

Association of the amino acid motifs of BoLA‐DRB3 alleles with mastitis pathogens in Japanese Holstein cows

“…This study was performed using the data obtained from a previous study (Yoshida et al. 2009) on BoLA‐DRB3 alleles identified by the PCR‐SBT method (Takeshima et al.…”

“…In our previous study (Yoshida et al. 2009), we investigated the association of the BoLA‐DRB3 alleles identified by the polymerase chain reaction sequence‐based typing (PCR‐SBT) method (Takeshima et al.…”

A genome-wide association study of variants associated with acquisition of Staphylococcus aureus bacteremia in a healthcare setting

“…Evidence that human genetic characteristics influence susceptibility to S. aureus infection include: 1) higher rates of S. aureus infections in distinct ethnic populations, including African Americans [3,7], New Zealand Maori [8], Pacific Islanders [8], Australian Aboriginals [9], and Canadian Aboriginal peoples [10]; 2) familial clusters of S. aureus infection [11]; 3) rare genetic conditions associated with susceptibility to S. aureus [12-14]; 4) the impact of host genetic variation on persistent S. aureus carriage [15]; and 5) variable susceptibility to S. aureus infections among inbred mice [16] and cattle [17]. …”

The Major Histocompatibility Complex in Bovines: A Review

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