Association of the amino acid motifs of BoLA‐DRB3 alleles with mastitis pathogens in Japanese Holstein cows

Abstract

The association of the polymorphism of bovine leukocyte antigen (BoLA-DRB3) genes, identified by the polymerase chain reaction sequence-based typing (PCR-SBT) method, with resistance and susceptibility to mastitis caused by Streptococci, coagulase-negative Staphylococci, Escherichia coli and Staphylococcus aureus was investigated. Blood samples for DNA extraction were collected from 170 Holstein cows (129 mastitis and 41 healthy cows) from 5 districts in Chiba prefecture, Japan. Susceptibility or resistance to the mastitis-causing pathogens was thought to vary by the presence of amino acid substitutions at the 9, 11, 13, and 30 positions. DRB3*0101 and DRB3*1501 had amino acid motifs of Glu(9), Ser(11), Ser(13), and Tyr(30), and they were considered to have susceptibility to all 4 mastitis pathogens. In contrast, DRB3*1101 and DRB3*1401 had amino acid motifs of Gln(9), His(11), Gly(13), and His(30) in these positions, and they also had Val(86), so these alleles were considered to have resistance to Streptococcal and coagulase-negative Staphylococcal mastitis. However, in the case of Escherichia coli mastitis, amino acid substitutions at the 9, 11, 13, and 30 positions had little effect, but rather substitutions at the 47, 67 positions of pocket 7, and at the 71, 74 positions of pocket 4, Tyr(47), Ile(67), Ala(71), and Ala(74), were associated with resistance. This motif was present in DRB3*1201.

Association Between BoLA-DRB3.2 Polymorphism and Bovine Papillomavirus Infection for Bladder Tumor Risk in Podolica Cattle

“…Indeed it has been shown that Arg 71 in P4 is correlated with resistance to persistent lymphocytosis caused by the bovine leukemia virus (42). Furthermore, Arg 71 in P4 has been associated with a high resistance to Escherichia coli mastitis in dairy cows (43). Our results appeared to strengthen previous studies suggesting that amino acid residues in P4 are crucial for resistance and/or susceptibility to diseases (34).…”

Major Histocompatibility Complex Class II (DRB3) Genetic Diversity in Spanish Morucha and Colombian Normande Cattle Compared to Taurine and Zebu Populations

“…The frequency of an MHC allele or group of alleles might become increased in cattle populations having the same selective pressures imposed by similar production systems, even in different geographic locations. From the analysis of previously published data (Takeshima et al, 2015a), we found that Holstein populations were enriched with alleles reported to be associated with resistance (BoLA-DRB3*011:01 and BoLA-DRB3*012:01) and susceptibility (BoLA-DRB3*001:01 and BoLA-DRB3*015:01) to mastitis (Yoshida et al, 2009b;Yoshida et al, 2009a). The Holstein breed has been submitted to intense selective pressure due to milk production traits which can make it susceptible to mastitis but, at the same time, there is also pressure for selecting resistant individuals.…”

A novel association of BoLA DRB3 alleles in BLV infected cattle with different proviral loads

“…On the other hand, other issues like production traits improvement, resistance to mastitis pathogens, somatic cell counts and milk yield have also been related to specific BoLA alleles (DIETZ et al, 1997;RUPP et al, 2007;YOSHIDA et al, 2009;BALTIAN et al, 2012;YOSHIDA et al, 2012). However, some controversies exist between different studies.…”

“…These genes are associated with genetic resistance and susceptibility to a wide range of diseases, including mastitis (DIETZ et al, 1997;RUPP et al, 2007;YOSHIDA et al, 2009;YOSHIDA et al, 2012), dermatophilosis (MAILLARD et al, 2002), tick-borne diseases (DUANGJINDA et al, 2013), infection with Neospora caninum (SCHWAB et al, 2009), African trypanosome species (KARIMURIBO et al, 2011), and Theileria annulata (GLASS et al, 2012b). Moreover, BoLA appears to influence other traits such as milk yield, growth and reproduction, and variations in immune responses to antigens (TAKESHIMA; AIDA, 2006) and even vaccine peptides (GARCIA-BRIONES et al, 2000;GLASS et al, 2012a).…”