1995
DOI: 10.1128/mcb.15.8.4232
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Association of Insulin Receptor Substrate 1 with Simian Virus 40 Large T Antigen

Abstract: Mouse embryo cells expressing a wild-type number of insulin-like growth factor I receptors (IGF-IR) (W cells) can be transformed either by simian virus 40 large T antigen (SV40 T) or by overexpressed insulin receptor substrate 1 (IRS-1), singly transfected. Neither SV40 T antigen nor IRS-1, individually, can transform mouse embryo cells with a targeted disruption of the IGF-IR genes (R ؊ cells). However, cotransfection of SV40 T antigen and IRS-1 does transform R؊ cells. In this study, using different antibodi… Show more

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Cited by 73 publications
(56 citation statements)
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“…Considering that both T-positive and T-negative cells have similar levels of the IGF-IR and P13-K proteins, and both grossly overexpress IRS-1 (Figure 3), it is feasible that the IGF-IR signaling pathway and JCV Tantigen may cooperate by amplifying the signal towards transformation in this cellular system. There are several reports that support this notion: JCV Tantigen has high homology to SV40 large T-antigen (Gallia et al, 1998); both JCV T-antigen and SV40 Tantigen have transforming properties (Gallia et al, 1998); SV40 T-antigen is not able to transform cells in the absence of IGF-IR (Sell et al, 1993); cells expressing functional IGF-IR can be transformed either by ectopic expression of SV40 T-antigen or by IRS-1 overexpression (Fei et al, 1995); IRS-1 coprecipitates with the SV40 T-antigen (Fei et al, 1995), and SV40 T-antigen enhances expression from the IGF-1 promoter (Porcu et al, 1994). Whether similar interactions exist between the IGF-IR system and the JCV T-antigen, and whether these interaction/s contribute to the development and progression of medulloblastoma remains to be determined.…”
Section: Discussionmentioning
confidence: 97%
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“…Considering that both T-positive and T-negative cells have similar levels of the IGF-IR and P13-K proteins, and both grossly overexpress IRS-1 (Figure 3), it is feasible that the IGF-IR signaling pathway and JCV Tantigen may cooperate by amplifying the signal towards transformation in this cellular system. There are several reports that support this notion: JCV Tantigen has high homology to SV40 large T-antigen (Gallia et al, 1998); both JCV T-antigen and SV40 Tantigen have transforming properties (Gallia et al, 1998); SV40 T-antigen is not able to transform cells in the absence of IGF-IR (Sell et al, 1993); cells expressing functional IGF-IR can be transformed either by ectopic expression of SV40 T-antigen or by IRS-1 overexpression (Fei et al, 1995); IRS-1 coprecipitates with the SV40 T-antigen (Fei et al, 1995), and SV40 T-antigen enhances expression from the IGF-1 promoter (Porcu et al, 1994). Whether similar interactions exist between the IGF-IR system and the JCV T-antigen, and whether these interaction/s contribute to the development and progression of medulloblastoma remains to be determined.…”
Section: Discussionmentioning
confidence: 97%
“…Conversely, reactivation of IRS-1 expression in cells from the external granular layer (Kulik et al, 1997;Reiss et al, 2000). Additionally, the IRS-1 protein has transforming properties but only when overexpressed in cells with a functional IGF-IR (Fei et al, 1995). In addition, the IRS-1 protein has been found to co-precipitate with the SV40 T antigen.…”
Section: Discussionmentioning
confidence: 99%
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“…Another viral oncoprotein, SV40T antigen, interacts directly with IRS-1 (Fei et al, 1995) relocalizing it to the nucleus. Transformation by SV40T requires IRS-1 to be phosphorylated on tyrosine residues to allow the activation of PI3 kinase, such that the absence or inactivation of IRS-1 renders cells refractory to transformation (DeAngelis et al, 2006).…”
Section: Discussionmentioning
confidence: 99%
“…S3). To test if SC66 could inhibit the Akt signaling pathway, HEK293T cells, which were shown to contain a high level of PIP3 (19), were treated with different amounts of SC66, and the whole-cell lysates were examined for the phosphorylation level of Akt and its known target proteins (Fig. 1B).…”
Section: Cell-based Screening Identifies a Compound That Directly Facmentioning
confidence: 99%