Association of interleukin-1B and interleukin-1RN polymorphisms with gastric cancer in a high-risk population of Costa Rica

Abstract

Several risk factors have been associated with gastric cancer, among them Helicobacter pylori infection. This bacterium yields inflammation, the degree of which depends on the bacterial strain and the severity of the host response. The inflammatory response involves a complex cytokine network. Recently, polymorphisms of the genes coding for interleukin-1beta (IL-1B), interleukin-1Ra (ILRN) and interleukin-10 have been associated with an increased risk of gastric cancer. In order to determine the association of the IL-1B, IL-1RN and IL-10 polymorphisms with gastric cancer in a high-risk Costa Rican population, we analysed purified DNA of 58 gastric cancer patients, 99 controls and 41 patients classified as group I or II, according to the Japanese classification. Genotyping was carried out by PCR, PCR-RFLP and pyrosequencing analysis. We did not find any association of the IL-1B-31, IL-1B-511 and IL-10 polymorphisms with the risk for developing gastric cancer in the studied population. Carriers of the IL-1B+3954T/- had an increased risk for developing gastric cancer (OR 3.7; 95%CI: 1.34-10.2). Also we found an increased risk for developing gastric cancer for allele 2 heterozygotes of the IL-1RN (OR 2.94; 95%CI: 1.09-7.93). This is the first time that IL-1B+3954 has been associated with gastric cancer. This is one of the first studies trying to describe the role played by IL-1B, IL-1RN and IL-10 genetic polymorphisms in gastric cancer in one of the highest risk American countries. Further investigation on American countries is needed.

Interleukin‐1B 31 C>T polymorphism combined with Helicobacter pylori‐modified gastric cancer susceptibility: evidence from 37 studies

“…Of the remaining 38 publications, three were eliminated, due to reporting duplicate data 17, 18, 19. Ultimately, 37 studies from 35 publications 6, 10, 14, 15, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50 including 6108 cases and 8980 controls were included in the meta‐analysis (Fig. 1).…”

A Meta-Analysis of Interleukin-10-1082 Promoter Polymorphism Associated with Gastric Cancer Risk

“…Since the year 2002, a lot of studies have focused on the IL-10-1082 G allele association with the susceptibility to gastric cancer, but with mixed or conflicting results (Wu et al, 2002;El-Omar et al, 2003;Wu et al, 2003;Savage et al, 2004;Alpízar-Alpízar et al, 2005;Guo et al, 2005;Lee et al, 2005;Lu et al, 2005;Zambon et al, 2005;Kamangar et al, 2006;Morgan et al, 2006;García-González et al, 2007;Sugimoto et al, 2007;Bai et al, 2008;Crusius et al, 2008;Deng et al, 2008;Zhou, et al, 2008a;Kang et al, 2009;Ko et al, 2009;Xiao et al, 2009;Liu et al, 2011;Zhou et al, 2011). Up to now, there has been just one relevant published meta-analysis regarding IL-10-1082 A/G SNP (Zhou et al, 2008b).…”

Relation of atrophic gastritis with Helicobacter pylori -CagA+ and interleukin-1 gene polymorphisms

“…The control group consisted of dyspeptic patients of which a large majority suffered from some inflammatory condition (NAG). In a previous study, performed by our group, among participants in a program at the Center for Early Detection of Gastric Cancer in Costa Rica, it was found that IL-1B + 3945T and IL-1RN*2 were associated with an increased risk of gastric cancer: OR = 3.7 P = 0.007 and OR = 2.9; P = 0.03 respectively [34] . In this study, the control groups were normal individuals as judged by X-ray (double contrast, gastric study).…”