Association of interleukin-8 polymorphism (+ 781 C/T) with the risk of oral Lichen Planus disease

Abstract

Background Oral Lichen Planus (OLP) is a chronic inflammatory mucosal disease. The pathogenesis of OLP is unknown. The Single Nucleotide Polymorphism (SNP) that occurs in the regulatory position + 781 could affect the expression of interleukin-8. This polymorphism is probably associated with increased serum levels of IL-8. The current study aimed to investigate the genotype and allele frequencies of IL-8( + 781 C/T) in OLP patients and whether it is associated with the severity of OLP disease in an Iranian population. Methods Three milliliters of saliva were taken from 100 patients with OLP and 100 healthy individuals who were matched in age and gender. After DNA extraction from saliva samples of patients and healthy individuals, the genotype of IL-8 at position + 781 is detected using the PCR-RFLP method. The results were analyzed using SPSS software. Results Frequency of C/C, T/C, and T/T genotypes at position IL-8 + 781 gene in the patient group were 47%, 41%, and 12%, respectively, and in the control group, were 37%, 42%, and 21%. The difference between the two groups regarding allele frequency distribution was statistically significant (χ2 = 3.86, p = 0.049, 95% CI = 0.44-1, OR = 0.66). Our results indicated the significantly higher frequency of the TT genotype in the erosive OLP compared to the nonerosive group (p = 0.03, OR = 0.89, 95% CI = 0.49–1.6). Conclusion This study depicted the difference in the frequency of SNP IL-8 + 781 C/T allele in the patient and control groups had a significant association with the risk of OLP. In addition, our data revealed that IL-8 + 781 C/T polymorphisms might be associated with the severity of OLP in the Iranian population.

Evaluation of the association between TNF-α-1031 T/C polymorphism with oral lichen planus disease

“…The sample size of ~ 93 was calculated for each group with an alpha value of 0.05 and a beta value of 20%, but to ensure sample adequacy, 100 subjects were enrolled in each of the case and control groups [ 24 ]. The OLP diagnosis was based on the definition of OLP by the World Health Organization [ 25 ] and confirmed by pathology reports and clinical manifestations detected by an oral medicine specialist.…”

Assessment of TNF‐α (‐857 C/T) gene polymorphism in oral lichen planus disease: A case–control study

“…Although it sometimes involves other places. Lesions are usually bilateral and often present as a combination of clinical symptoms 2,3 . OLP occurs more than the cutaneous form.…”

“…Salivary TNF‐α levels increase in the histological differentiation grade in OSCC 11 . Studies show that factors such as stress, drugs, genetics, dental materials, liver diseases, hepatitis C virus infection, tobacco, and alcohol can be effective in causing OLP 5,12–14 . OLP is an autoimmune T‐cell‐mediated disease, but its cause is often unknown.…”

“…Lesions are usually bilateral and often present as a combination of clinical symptoms. 2 , 3 OLP occurs more than the cutaneous form. It is also more lasting and resistant to treatment.…”

“… 11 Studies show that factors such as stress, drugs, genetics, dental materials, liver diseases, hepatitis C virus infection, tobacco, and alcohol can be effective in causing OLP. 5 , 12 , 13 , 14 OLP is an autoimmune T‐cell‐mediated disease, but its cause is often unknown. There is a theory about immune responses mediated by T cells that play a role in it.…”