Association of macrophages detected with monoclonal antibody 25 F 9 with progression and pathobiological classification of gastric carcinoma

Abstract

Using monoclonal antibody 25 F 9, which reacts with a determinant of mature macrophages, the inflammatory infiltrate of 66 gastric carcinomas was evaluated using a counting grid. The ratio tumor cells/macrophages was determined for every tumor. For a threshold value of 5, carcinomas with a better prognosis, such as the intestinal type according to Lauré, the expanding type according to Ming and the differentiated carcinomas according to the WHO had a significantly smaller relative content of 25 F 9-positive macrophages (a minimum of P less than 0.05) than the diffuse type, infiltrative type, and undifferentiated carcinomas. Furthermore, the relative macrophage content tended to increase with the stage of carcinomas spread (P less than 0.1). The results suggested that 25 F 9-positive macrophages in gastric carcinoma are of greater significance in tumor spread than in any defensive reaction against the tumor.

Elevated tumour interleukin-1β is associated with systemic inflammation: a marker of reduced survival in gastro-oesophageal cancer

“…The prognostic significance of tumour-associated inflammatory cells is less clear in gastro-oesophageal cancer. An increased macrophage infiltrate was associated with more advanced stage of disease among patients with gastric cancer in one study, whereas other studies have suggested a more favourable prognosis associated with a more pronounced macrophage infiltration (Heidl et al, 1987;Tsujitani et al, 1987;Ohno et al, 2003). Similarly, increasing tumour-infiltrating lymphocyte count has been linked with decreased risk of death from gastric cancer in one study, but associated with an adverse prognosis in another (Setala et al, 1996;Grogg et al, 2003).…”

The Role of Hypoxia in Re-educating Macrophages in the Tumour Environment

“…Macrophage accumulation in tumours is known to correlate with poor patient prognosis in the majority of cancer types (Fujimoto et al, 2000;Hamada et al, 2002;Hanada et al, 2000;Heidl et al, 1987;Leek et al, 1996;Lissbrant et al, 2000;Salvesen and Akslen, 1999). This can be explained by the tumour-promoting phenotype of TAMs, induced by the tumour microenvironment.…”

“…A positive correlation that is also seen between hypoxia and TAM numbers in secondary liver tumours that form as metastases from breast and colorectal tumours (Stessels et al, 2004). There is an inverse relationship between TAM infiltration and patient prognosis seen in many human cancers (Fujimoto et al, 2000;Hamada et al, 2002;Hanada et al, 2000;Heidl et al, 1987;Leek et al, 1996;Lissbrant et al, 2000;Salvesen and Akslen, 1999), which implies that these macrophages adopt a pro-tumoural phenotype, contrasting with their more classic role as pathogen and tumour killing cells and with their ability to initiate an immune response.…”

“…Subsequently, a wide panel of protumour genes are upregulated by hypoxic macrophages which could support tumour growth, survival and metastasis (Fang et al, 2009). This is thought to explain the correlation between high numbers of TAMs and poor patient prognosis in many types of human tumours (Fujimoto et al, 2000;Hamada et al, 2002;Hanada et al, 2000;Heidl et al, 1987;Leek et al, 1996;Lissbrant et al, 2000;Salvesen and Akslen, 1999).…”

Squamous cell carcinoma: infiltrating monocyte/macrophage subpopulations express functional mature phenotype

“…Other authors found that in malignant melanomas and gastric carcinomas 27E1O0 and 25F9+ macrophages were associated with tumour progression (Brocker et al, 1987;Heidl et al, 1987;Brocker et al, 1988). In acute gingival inflammation the dominant Mo/MO population carried the 27E10 antigen, whereas the numbers of Rm3/1 and 25F9 positive cells were low (Zwadlo et al, 1985(Zwadlo et al, , 1986.…”