Association of myocardial and serum miRNA expression patterns with the presence and extent of coronary artery disease: A cross-sectional study

Polyakova, E.; Зарайский, М. И.; Mikhaylov, Evgeny N.; Баранова, Е. И.; Галагудза, М. М.; Shlyakhto, E.

doi:10.1016/j.ijcard.2020.08.043

Cited by 16 publications

(12 citation statements)

References 18 publications

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“…Additionally, many miRNAs are important in the regulation of atherosclerotic plaque formation [ 188 ]. The cross-sectional observational study with 100 subjects showed an increase in miRNA-133 level in the patients with CAD compared with the control group [ 189 ], and the miRNA-133 level was increased in symptomatic plaques [ 190 ]. In addition, inhibition of miRNA-133 can target NOS to prevent endothelial dysfunction [ 191 ].…”

Section: Therapies For Oxidative Stress-associated Cardiovascular mentioning

confidence: 99%

Oxidative Stress and Antioxidant Treatments in Cardiovascular Diseases

2020

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Oxidative stress plays a key role in many physiological and pathological conditions. The intracellular oxidative homeostasis is tightly regulated by the reactive oxygen species production and the intracellular defense mechanisms. Increased oxidative stress could alter lipid, DNA, and protein, resulting in cellular inflammation and programmed cell death. Evidences show that oxidative stress plays an important role in the progression of various cardiovascular diseases, such as atherosclerosis, heart failure, cardiac arrhythmia, and ischemia-reperfusion injury. There are a number of therapeutic options to treat oxidative stress-associated cardiovascular diseases. Well known antioxidants, such as nutritional supplements, as well as more novel antioxidants have been studied. In addition, novel therapeutic strategies using miRNA and nanomedicine are also being developed to treat various cardiovascular diseases. In this article, we provide a detailed description of oxidative stress. Then, we will introduce the relationship between oxidative stress and several cardiovascular diseases. Finally, we will focus on the clinical implications of oxidative stress in cardiovascular diseases.

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Section: Therapies For Oxidative Stress-associated Cardiovascular mentioning

confidence: 99%

Oxidative Stress and Antioxidant Treatments in Cardiovascular Diseases

2020

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“…Since a variety of miRNAs are often present during the onset of AMI with extremely low levels, extending the detection object to a group composed of multiple related miRNAs or any related miRNA in the group can result in a faster and earlier diagnosis of the disease. − To better simulate actual conditions, we extended the detection range of our sensor to the group composed of multiple myocardial miRNAs. We performed selective tests on simulated cohorts composed of two myocardial miRNAs and cohorts composed of three myocardial miRNAs (Figure C,D).…”

Section: Resultsmentioning

confidence: 99%

A Multitargeted Electrochemiluminescent Biosensor Coupling DNAzyme with Cascading Amplification for Analyzing Myocardial miRNAs

et al. 2021

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Several circulating miRNAs are associated with the pathogenic process of acute myocardial infarction (AMI). Thus, analyzing myocardial miRNAs in the circulatory system is important for the diagnosis and treatment of AMI, especially for early-stage diagnosis. Based on the characteristics of myocardial miRNAs, an ultrasensitive and multitargeted electrochemiluminescence (ECL) sensing platform was developed with a versatile probe that can couple DNAzyme with hybridization chain reaction amplification. The target miRNA and auxiliary chains form a circular unit that shears the versatile probe hairpin, and the products subsequently trigger cascading amplification; a long strand of dsDNA is then generated with many C-rich sequences that can undergo in situ reductions to generate ECL luminophore silver clusters. Using this strategy, three myocardial miRNAs are successfully detected with a detection limit as low as 29.6 aM (S/N = 3). Notably, our method can detect myocardial miRNA groups composed of multiple related circulating miRNAs with high selectivity over interfering miRNAs in blood. This is extremely important for solving the problem of diverse and low abundance of infarct-associated miRNAs. Our strategy pioneers a new idea of miRNA detection, and given its versatility and sensitivity, it is promising for the diagnosis of multigene-regulated cardiovascular diseases.

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“…Kemal Marc Akat et al 27 acclaimed miR-203 downregulation in heart failure. However, E.A.Polyakova et al 28 noticed miRNA-203 overexpression in serum and cardiomyocytes of patients with multiple coronary artery stenosis compared to controls. Zhi and colleagues have reported the increased levels of miR-203 in the rat heart under hypoxia 29 .…”

Section: Discussionmentioning

confidence: 98%

Peripheral blood mononuclear cells expression of miR-200c, miR-125b, miR-27b, miR-203, and miR-155 in patients with significant or insignificant coronary artery stenosis

Saadatian,

Mansoori,

Nariman-Saleh-Fam

et al. 2023

Sci Rep

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Coronary artery disease (CAD) is one of the principal causes of death worldwide. Among several predisposing factors, inflammation and inflammatory genes play a significant role in disease pathogenesis. Inflammatory microRNAs, small noncoding RNAs involved in regulating inflammation, are promising candidates for understanding pathogenesis of CAD and developing diagnostic biomarkers. The aim of the study was to evaluate the alteration of miR-200c, miR-125b, miR-27b, miR-203 and, miR-155 in patients suffering from coronary artery stenosis and insignificant coronary artery stenosis compared to healthy subjects. In this study we compared expressions of five inflammatory miRNAs in peripheral blood mononuclear cells (PBMCs) of 72 patients suffering significant coronary artery stenosis (CAD), 74 individuals without coronary artery disease and 30 individuals with insignificant coronary artery stenosis (ICAD). After blood collection, PBMCs were isolated and RNA was extracted. Gene expression levels were assessed by SYBR green based real-time PCR. Statistical analysis was performed using R program. Expression levels of miR-200c, miR-203, and miR-155 were lower in subjects with ICAD than that in CAD patients and subjects of the control group. MiR-125b was downregulated in CAD and ICAD groups compared to the control group. PBMC miR-27b was upregulated in the CAD group as compared to the ICAD and control groups. Receiver operating characteristic curve analysis verified potential of three miRNAs in separating subjects with ICAD from CAD patients and healthy individuals. In conclusion, this original investigation suggested that altered expression of these five miRNAs may serve as a novel diagnostic biomarker discriminating clinical presentations of coronary artery diseases.

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Association of myocardial and serum miRNA expression patterns with the presence and extent of coronary artery disease: A cross-sectional study

Cited by 16 publications

References 18 publications

Oxidative Stress and Antioxidant Treatments in Cardiovascular Diseases

Oxidative Stress and Antioxidant Treatments in Cardiovascular Diseases

A Multitargeted Electrochemiluminescent Biosensor Coupling DNAzyme with Cascading Amplification for Analyzing Myocardial miRNAs

Peripheral blood mononuclear cells expression of miR-200c, miR-125b, miR-27b, miR-203, and miR-155 in patients with significant or insignificant coronary artery stenosis

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