Association of oral Epstein‑Barr virus with periodontal health in Japanese adults

Su, Cheng-Yih; Shigeishi, Hideo; Murodumi, Hiroshi; Sugiyama, Masaru; Ohta, Kouji; Takemoto, Tsuyoshi

doi:10.3892/etm.2021.10199

Cited by 8 publications

(9 citation statements)

References 27 publications

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“…Several studies have demonstrated an association between oral health and EBV infection, as well as a strong association of EBV with periodontitis [ 56 ]. Inflamed periodontal pockets may be a reservoir for the EBV virus, which will then infect oral epithelial cells.…”

Section: Discussionmentioning

confidence: 99%

Could MMP3 and MMP9 Serve as Biomarkers in EBV-Related Oropharyngeal Cancer

Polz,

Morshed,

Drop

et al. 2024

IJMS

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The high incidence of, and mortality from, head and neck cancers (HNCs), including those related to Epstein–Barr virus (EBV), constitute a major challenge for modern medicine, both in terms of diagnosis and treatment. Therefore, many researchers have made efforts to identify diagnostic and prognostic factors. The aim of this study was to evaluate the diagnostic usefulness of matrix metalloproteinase 3 (MMP 3) and matrix metalloproteinase 9 (MMP 9) in EBV positive oropharyngeal squamous cell carcinoma (OPSCC) patients. For this purpose, the level of these MMPs in the serum of patients with EBV-positive OPSCC was analyzed in relation to the degree of histological differentiation and TNM classification. Our research team’s results indicate that the level of both MMPs is much higher in the EBV positive OPSCC patients compared to the EBV negative and control groups. Moreover, their levels were higher in more advanced clinical stages. Considering the possible correlation between the level of MMP 3, MMP 9 and anti-EBV antibodies, and also viral load, after statistical analysis using multiple linear regression, their high correlation was demonstrated. The obtained results confirm the diagnostic accuracy for MMP 3 and MMP 9. Both MMPs may be useful in the diagnosis of EBV positive OPSCC patients.

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Section: Discussionmentioning

confidence: 99%

Could MMP3 and MMP9 Serve as Biomarkers in EBV-Related Oropharyngeal Cancer

Polz,

Morshed,

Drop

et al. 2024

IJMS

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“…Thus, it is controversial whether HHV-7 is closely associated with periodontitis. We had previously found that EBV and CMV were significantly associated with the presence of a deep periodontal pocket with bleeding [ 3 , 23 ]. Therefore, oral herpesviruses such as EBV and HCMV, rather than HHV-7, may be more closely associated with active periodontitis.…”

Section: Discussionmentioning

confidence: 99%

Associations between Oral Human Herpesvirus-6 and -7 and Periodontal Conditions in Older Adults

Hamada

Shigeishi

Oka

et al. 2023

Life

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Background: The associations between oral human herpesvirus-6 (HHV-6) and HHV-7, periodontal conditions, and lifestyle-related diseases, such as hypertension, diabetes, and dyslipidemia, have not been fully investigated in older adults. Methods: Seventy-four older patients who visited Hiroshima University Hospital were enrolled. Tongue swab samples were employed, and a real-time polymerase chain reaction was performed to detect HHV-6 and HHV-7 DNA. Dental plaque accumulation, probing pocket depth, and bleeding on probing (BOP) (i.e., a sign of periodontal inflammation) were examined. The periodontal inflamed surface area (PISA) value (i.e., an indicator of the severity of periodontitis) was also examined. Results: Of the 74 participants, one participant (1.4%) was HHV-6 DNA-positive and 36 participants (48.6%) were HHV-7 DNA-positive. A significant association between HHV-7 DNA and probing depth was found (p = 0.04). The HHV-7 DNA-positive participants had a higher positive rate of a ≥ 6-mm periodontal pocket with BOP (25.0%) than the HHV-7 DNA-negative participants (7.9%). Additionally, the HHV-7 DNA-positive participants had a higher PISA value than the HHV-7 DNA-negative participants. However, there was no significant association between HHV-7 and the PISA value (p = 0.82). No significant association was found between HHV-7 and lifestyle-related diseases (p > 0.05). Conclusions: Oral HHV-7 infection is associated with a deep periodontal pocket.

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“…[5] We previously reported that patients with deep periodontal pockets with bleeding exhibited a greater oral EBV DNA positivity rate than did those without deep periodontal pockets with bleeding, indicating a significant relationship between oral EBV infection and periodontitis. [6] These results suggest that oral EBV is importantly involved in the etiology of periodontitis. Furthermore, interaction between EBV and periodontitis-related bacteria may contribute to periodontitis development and progression.…”

Section: Introductionmentioning

confidence: 93%

“…[12] A 180-bp DNA fragment of the EBV genome cloned into a pUC57 vector was obtained from GenScript Biotech Corporation (Piscataway, NJ). [6] Standard curves for 10-fold serial dilutions of the EBV genome inserted into a pUC57 vector (1.55 × 10 7 -1.55 × 10 2 copy/μL) were used to determine EBV-positive samples (Fig. S2, http://links.lww.com/MD/H707).…”

Section: Detecting Ebv Dna and P Gingivalis By Real-time Pcrmentioning

confidence: 99%

Prevalence of oral Epstein-Barr virus and Porphyromonas gingivalis and their association with periodontal inflamed surface area: A cross-sectional study

Shigeishi

Oka

et al. 2022

Medicine

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We previously reported that oral herpesviruses, such as Epstein-Barr virus (EBV), are associated with periodontitis. However, the relationship between oral EBV or dual oral EBV and Porphyromonas gingivalis infections and periodontal inflammation severity remains unclear. We conducted this study to determine the relationship between oral EBV and P gingivalis prevalence and the periodontal inflamed surface area (PISA) in middle-aged and older adults. We analyzed 205 patients (median age, 70 years) who visited Hiroshima University Hospital. Tongue swab samples were used to investigate the presence of EBV and P gingivalis DNA using real-time PCR. Probing pocket depth and bleeding on probing were measured at 6 sites per tooth. PISA scores were calculated based on the results of probing pocket depth and bleeding on probing. Propensity scores were calculated via logistic regression analysis of 8 clinical factors: age, sex, smoking status, remaining teeth, denture use, hypertension, diabetes, and hyperlipidemia. EBV DNA was present in 41 of the 205 participants (20.0%). Thirty-seven EBV-positive or -negative participants in 74 matched pairs after propensity-score matching were examined via univariate analysis. EBV-positive participants exhibited higher plaque control record scores and PISAs than did EBV-negative participants. EBV DNA was significantly associated with plaque control record scores and PISA (both P = .04). Of the 205 participants, 111 were positive for P gingivalis (54.1%). Nineteen participants (9.3%) were infected with both oral EBV and P gingivalis. Logistic regression analysis revealed that dual infection with EBV and P gingivalis was significantly associated with diabetes (odds ratio = 3.37, 95% confidence interval: 1.13-10.1; P = .03). Oral EBV prevalence is associated with oral hygiene and the spread of inflamed periodontal tissue. Diabetes may be a risk factor for dual infection with oral EBV and P gingivalis.Abbreviations: EBV = Epstein-Barr virus, PCR = polymerase chain reaction, PESA = periodontal epithelial surface area, PISA = periodontal inflamed surface area.

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Association of oral Epstein‑Barr virus with periodontal health in Japanese adults

Cited by 8 publications

References 27 publications

Could MMP3 and MMP9 Serve as Biomarkers in EBV-Related Oropharyngeal Cancer

Could MMP3 and MMP9 Serve as Biomarkers in EBV-Related Oropharyngeal Cancer

Associations between Oral Human Herpesvirus-6 and -7 and Periodontal Conditions in Older Adults

Prevalence of oral Epstein-Barr virus and Porphyromonas gingivalis and their association with periodontal inflamed surface area: A cross-sectional study

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