Associations between Oral Human Herpesvirus-6 and -7 and Periodontal Conditions in Older Adults

Hamada, Natsuki; Shigeishi, Hideo; Oka, Iori; Sasaki, M.; Kitasaki, Honami; Nakamura, Mariko; Yano, Kanako; Wu, Chhi‐Chong; Kaneyasu, Yoshino; Maehara, Tomoko; Sugiyama, Masaru; Ohta, Kouji

doi:10.3390/life13020324

Cited by 10 publications

(6 citation statements)

References 29 publications

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“…[ 59 , 60 ]. In the oral cavity, it has been suggested that HHV-7 is associated with active periodontitis [ 61 ].…”

Section: Discussionmentioning

confidence: 99%

Detection of Human Herpesviruses in Sera and Saliva of Asymptomatic HIV-Infected Individuals Using Multiplex RT-PCR DNA Microarray

Sufiawati,

Harmiyati,

Nur’aeny

et al. 2023

Pathogens

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Human herpesviruses (HHVs) are frequently linked to an increased risk of acquiring human immunodeficiency virus (HIV), and vice versa. This study aimed to detect human herpesvirus (HHV) members in the sera and saliva of asymptomatic HIV-infected individuals. Paired saliva and serum samples were obtained from 30 asymptomatic HIV-infected individuals. HHVs were detected with a multiplex reverse transcription-polymerase chain reaction (RT-PCR) DNA microarray Clart®Entherpex kit. A total of 30 subjects were enrolled: 23 (76.67%) men and 7 (23.33%) women. The present study showed that at least one or more HHV members were detected in the saliva and sera of all (100%) of the subjects. In the saliva, we detected herpes simplex virus 1 (HSV-1) 6.67%, herpes simplex virus 2 (HSV-2) 6.67%, Epstein–Barr virus (EBV) 86.67%, cytomegalovirus (CMV) 63.33%, HHV-6 (40%), and HHV-7 (83.33%). In the sera, HSV-2 (20%), EBV (30%), CMV (40%), HHV-6 (0%), and HHV-7 (76.67%) were found, but not HSV-1. VZV and HHV-8 were not detected in either the saliva or sera. EBV and HHV6 were significantly more prevalent in the saliva than they were in the sera of asymptomatic HIV-infected individuals (p < 0.05). However, no significant differences were found in the prevalence of HSV-1, EBV, CMV, HHV-6, and HHV-7 in the saliva and sera of asymptomatic HIV-infected individuals (p > 0.05). In conclusion, the multiplex RT-PCR DNA microarray can serve as a valuable diagnostic tool that can be used as a screening tool or a first-line test for HHVs infections.

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“…[ 59 , 60 ]. In the oral cavity, it has been suggested that HHV-7 is associated with active periodontitis [ 61 ].…”

Section: Discussionmentioning

confidence: 99%

Detection of Human Herpesviruses in Sera and Saliva of Asymptomatic HIV-Infected Individuals Using Multiplex RT-PCR DNA Microarray

Sufiawati,

Harmiyati,

Nur’aeny

et al. 2023

Pathogens

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“…Our previous study reported that oral EBV has a prevalence rate of 20% and is associated with PISA and plaque control record scores (37). Additionally, oral human herpesvirus-7 is commonly found in individuals with deep periodontal pockets (38). However, the association between Capnocytophaga species and periodontal herpes virus remains unclear.…”

Section: Ochracea/c Sputigenamentioning

confidence: 99%

Prevalence of oral Capnocytophaga species and their association with dental plaque accumulation and periodontal inflammation in middle‑aged and older people

Shigeishi,

Hamada,

Kaneyasu

et al. 2024

Biomed Rep

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Capnocytophaga species are commonly found in human oral microbiome. The aim of the present study was to understand the association of the prevalence of oral Capnocytophaga species with oral hygiene and periodontal inflammation. A total of 136 patients (median age 72 years) who visited the Hiroshima University Hospital (Hiroshima, Japan) between April 2021 and June 2023 were enrolled. Swab samples were obtained from the tongue surface. DNA from Capnocytophaga species (C. ochracea and C. sputigena) was detected by real-time PCR analysis. Dental plaque accumulation was observed to assess the oral hygiene condition of participants. Additionally, clinical periodontal inflammation was assessed with periodontal inflamed surface area (PISA) scores. Clinical confounding factors such as age, sex, lifestyle-related disease, remaining teeth and denture wearing between Capnocytophaga species-positive and -negative groups were adjusted with a propensity score matching method. Mann-Whitney U and χ 2 or Fisher's exact test were employed for statistical analysis. The prevalence rate was 67.6% for oral C. ochracea and 83.1% for C. sputigena. C. ochracea-positive participants showed significantly higher plaque control record scores (an indicator of dental plaque accumulation) than C. ochracea-negative participants (P= 0.03). Additionally, C. ochracea/C. sputigena dual-positive participants exhibited significantly higher plaque control record and PISA scores than non-dual-positive participants (P= 0.01 and P= 0.04, respectively). Propensity score matching was conducted in the C. ochracea/C. sputigena dual-positive group and the non-dual-positive group for adjustment of clinical factors, resulting in 51 matched patient pairs. C. ochracea/C. sputigena dual-positive participants had significantly higher plaque control record scores than non-dual-positive participants (P= 0.02). The present results suggest that the prevalence of both oral C. ochracea and C. sputigena is associated with poor oral hygiene in middle-aged and older people.

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“…In fact, CMV seroprevalence was highest in South America, Africa, and Asia, and lowest in Western Europe [29]. Moreover, few studies have addressed the molecular identi cation of the herpesviruses after the updates of the new Classi cation of Periodontal and Peri-Implant Diseases [31,32], and to the authors knowledge, none have compared CMV and EBV occurrence in patients with periodontitis in the Spanish population.…”

Section: Introductionmentioning

confidence: 99%

Detection of Epstein-Barr Virus and Cytomegalovirus in patients with periodontitis: a case-control study.

Darbinyan,

Herrero-Sánchez

2024

Preprint

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Background: Opportunistic viruses such as cytomegalovirus (CMV) and Epstein-Barr Virus (EBV) have been detected in gingival crevicular fluid (GCF) and saliva of patients with periodontal disease (PD). However, the relationship between herpesviruses and PD etiology and progression remains obscure. The aim of this case-control study was the detection and association of CMV and EBV with PD. Material and methods: Forty-eight adults were included in this study: twenty-four patients with periodontitis and twenty-four periodontally healthy individuals. All patients underwent periodontal examination including probing depth, clinical attachment loss, plaque index, and bleeding on probing. Subgingival biofilms samples were collected from all patients and real-time PCR were performed for viral and bacterial detection. The odds ratio (OR) was calculated, and the chi-squared test or Fisher’s exact test was performed to analyse the significant differences. Results: EBV was detected only in one healthy patient meanwhile no CMV was found. With exception of A.Actinomycetemcomitans, P.gingivalis, T. forsythia, P. intermedia and T.denticola showed a higher detection rate (p< 0.001) in periodontal sites. Conclusion: the present case-control study showed that the prevalence of EBV and CMV did not show significant differences in the groups evaluated in the Spanish population. Clinical Relevance: in this specific population, EBC and CMV does not seem to play a role in the aetiology of periodontitis, hence,

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Associations between Oral Human Herpesvirus-6 and -7 and Periodontal Conditions in Older Adults

Cited by 10 publications

References 29 publications

Detection of Human Herpesviruses in Sera and Saliva of Asymptomatic HIV-Infected Individuals Using Multiplex RT-PCR DNA Microarray

Detection of Human Herpesviruses in Sera and Saliva of Asymptomatic HIV-Infected Individuals Using Multiplex RT-PCR DNA Microarray

Prevalence of oral Capnocytophaga species and their association with dental plaque accumulation and periodontal inflammation in middle‑aged and older people

Detection of Epstein-Barr Virus and Cytomegalovirus in patients with periodontitis: a case-control study.

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