Association of Patatin-Based Proteomic Distances with Potato (Solanum tuberosum L.) Quality Traits

Abstract

Patatin is the major tuber storage protein constituted by multiple isoforms highly variable across potato (S. tuberosum) varieties. Here, we report a first association study of the variability of patatin isoforms between cultivars with their differences in tuber quality traits. Patatin-based proteomic distances were assessed between 15 table and/or processing potato cultivars from profiles of patatin obtained by two-dimensional electrophoresis. The content of ash, dry matter, reducing sugars, starch, total protein, and amino acid composition was also evaluated in tubers of each cultivar. Results showed that proteomic distances were significantly (P < 0.05) associated with differences in the content of ash, dry matter, and essential amino acids. Proteomic distances were also able to identify outlier cultivars regarding the content of dry matter, content of protein, and protein quality. In conclusion, patatin-based proteomic distances can shorten the screening and selection processes of potato cultivars with advantageous characteristics in molecular breeding.

The Major Storage Protein in Potato Tuber Is Mobilized by a Mechanism Dependent on Its Phosphorylation Status

“…High-resolution patatin profiles from protein extracts were obtained using previously described targeted 2-DE protocols [23,24]. Briefly, first-dimension isoelectric focusing (IEF) was conducted on a PROTEAN ® IEF Cell (Bio-Rad Laboratories, Hercules, CA, USA) system using 24-cm-long immobilized pH gradient (IPG) strips (ReadyStrips™, Bio-Rad Laboratories) with linear pH gradient (pH 4–7).…”

“…Phosphorylated isoforms of VSPs were identified for the first time in the patatin protein [23]. It is a very complex protein that comprises multiple differentially phosphorylated isoforms within—and between—potato cultivars [23,24]. A 2-DE-based phosphoproteomic study enabled the identification of 20 differentially phosphorylated patatin isoforms in Kennebec cultivar, with phosphorylation rates ( PR s) varying between 4.6 and 52.3% [23].…”

“…For this purpose, a comprehensive analysis of temporal phosphorylation changes in patatin was performed in tubers of Kennebec cultivar (Figure 1). We used targeted 2-DE-based proteomic methods enabling the separation of multiple storage protein isoforms with high resolution and reproducibility as well as the efficient in-gel quantitation of their phosphorylation levels [8,17,23,24,26,27]. The results will contribute to understanding the mechanisms underlying the mobilization of VSPs.…”

Measuring quantitative proteomic distance between Spanish beef breeds

“…In particular, the proteomic distance between a given pair of populations can undergo significant variations due to the enormous spatio-temporal plasticity of the proteome. To date, only a few studies have used proteomic distance measurements to assess the relationship of proteomic differentiation between populations with intraspecific phylogenetic diversity established by genetic markers in the parasite Trypanosoma cruzi (Telleria et al, 2010), genetic proximities between species and genus of the plant family Brassicaceae (Marquès, Sarazin, Chané-Favre, Zivy, & Thiellment, 2001), variations in quality traits in the common bean (Phaseolus vulgaris) and potato (Solanum tuberosum) (López Pedrouso, Bernal, Franco, & Zapata, 2014;Mouzo, López-Pedrouso, Bernal, García, Franco, & Zapata, 2018), and changes in beef tenderness (Gagaoua et al, 2019). Proteomic distances were assessed using Jaccard's and Nei and Li's distance measures (Jaccard, 1908;Nei & Li, 1979).…”