Association of polymorphisms in AGTR1 and AGTR2 genes with primary aldosteronism in the Chinese Han population

Abstract

Hypothesis: Polymorphisms in angiotensin II type-1/2 receptor genes (AGTR1/AGTR2) may be involved in the pathogenesis of primary aldosteronism. The present study aims to reveal some loci susceptible to the disease on the genes in a group of Chinese Han nationality. Materials and methods: A case-control study was conducted in 202 patients and 188 controls. Ten tagging SNPs on AGTR1/AGTR2 were genotyped for all subjects via the method of multiplex PCR-ligase detection reaction. Statistical analysis was performed with chi-square test and logistic regression analysis. Results: rs3772616 on the AGTR1 gene was a factor for susceptibility to primary aldosteronism (p<0.001), and the TT genotype significantly decreased the risk of primary aldosteronism compared with the CC homozygote (p=0.008, adjusted OR=0.13; 95%CI: 0.03–0.59). The rs3772616 polymorphism was associated with primary aldosteronism under the additive and dominant models. The female carriers of the G allele in rs5193 showed a significant difference compared with the T allele. Conclusions: The AGTR1 rs3772616 polymorphism can be considered as a hereditary marker for primary aldosteronism, and in the Chinese Han population the rs5193 G allele seems to predispose to it only in women.

Correlation Between Angiotensin Receptor Type 1 Polymorphisms and Atherosclerotic Cerebral Infarction Risk

“…A previous study reported a possible link between the ACE2 rs6632677 and AT1R rs3772616 polymorphisms in patients with structural atrial fibrillation. 18 Wu et al 17 demonstrated that the AT1R rs3772616 polymorphism was associated with primary aldosteronism, the most prevalent type of secondary hypertension. Overall, our findings highlighted the strong relationship between the AT1R rs3772616 polymorphism and enhanced risk of developing ACI.…”

“…Genomic DNA was isolated from the peripheral blood of each participant with the help of a EZ-10 Spin Column Whole Blood Genomic DNA Isolation Kit (Tiangen, Beijing, China). AT1R polymorphisms were selected, based on published studies, 17–20 with genotyping (detailed primers enlisted in Table 1 ) conducted via the improved multiplex PCR-ligase detection reaction (iMLDR-TM) developed by Genesky Biotechnologies, Inc. (Shanghai, China). 21 …”