Association of RGA-SSCP markers with resistance to downy mildew and anthracnose in grapevines

Tantasawat, Piyada Alisha; Poolsawat, O.; Prajongjai, T.; Chaowiset, W.; Tharapreuksapong, Akkawat

doi:10.4238/2012.july.2.1

Cited by 11 publications

(8 citation statements)

References 33 publications

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“…The symptoms were assessed in variable times of incubation. The results obtained by these assays were consistent and were applied for pathogenicity analysis (Tharapreuksapong et al, 2009; Santos et al, 2018a), germplasm characterization (Kono et al, 2012) and genetic analysis (Tantasawat et al, 2012; Poolsawat et al, 2013).…”

Section: Disease Descriptionsupporting

confidence: 54%

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Emergent Ascomycetes in Viticulture: An Interdisciplinary Overview

et al. 2019

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The reduction of pesticide usage is a current imperative and the implementation of sustainable viticulture is an urgent necessity. A potential solution, which is being increasingly adopted, is offered by the use of grapevine cultivars resistant to its main pathogenic threats. This, however, has contributed to changes in defense strategies resulting in the occurrence of secondary diseases, which were previously controlled. Concomitantly, the ongoing climate crisis is contributing to destabilizing the increasingly dynamic viticultural context. In this review, we explore the available knowledge on three Ascomycetes which are considered emergent and causal agents of powdery mildew, black rot and anthracnose. We also aim to provide a survey on methods for phenotyping disease symptoms in fields, greenhouse and lab conditions, and for disease control underlying the insurgence of pathogen resistance to fungicide. Thus, we discuss fungal genetic variability, highlighting the usage and development of molecular markers and barcoding, coupled with genome sequencing. Moreover, we extensively report on the current knowledge available on grapevine-ascomycete interactions, as well as the mechanisms developed by the host to counteract the attack. Indeed, to better understand these resistance mechanisms, it is relevant to identify pathogen effectors which are involved in the infection process and how grapevine resistance genes function and impact the downstream cascade. Dealing with such a wealth of information on both pathogens and the host, the horizon is now represented by multidisciplinary approaches, combining traditional and innovative methods of cultivation. This will support the translation from theory to practice, in an attempt to understand biology very deeply and manage the spread of these Ascomycetes.

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Section: Disease Descriptionsupporting

confidence: 54%

“…However, no information was found about the use of the SCAR in routine breeding programs to assist the introgression of the resistant gene into V. vinifera germplasm. Recently, also RGA-SSCP markers have been found associated with AN resistance (Tantasawat et al, 2012).…”

Section: Grapevine-ascomycete Interactionmentioning

confidence: 99%

Emergent Ascomycetes in Viticulture: An Interdisciplinary Overview

et al. 2019

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“…Recently, we found three resistance gene analog (RGA)-single-strand conformation polymorphism (SSCP) markers that were associated with downy mildew and anthracnose resistance in crosses using 'NY65.0550.04' as a resistance source. These markers could be used to accelerate the selection process for improved downy mildew and anthracnose resistance, particularly in the 'Carolina Black Rose x NY65.0550.04' cross in future breeding programs (Tantasawat et al, 2012).…”

Section: Anthracnose Resistancementioning

confidence: 99%

Inheritance of downy mildew (Plasmopara viticola) and anthracnose (Sphaceloma ampelinum) resistance in grapevines

Poolsawat

Mahanil²,

Laosuwan

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Downy mildew (Plasmopara viticola) and anthracnose (Sphaceloma ampelinum) are two of the major diseases of most grapevine (Vitis vinifera L.) cultivars grown in Thailand. Therefore, breeding grapevines for improved downy mildew and anthracnose resistance is crucial. Factorial crosses were made between three downy mildew and/or anthracnose resistant lines ('NY88.0517.01', 'NY65.0550.04', and 'NY65.0551.05'; male parents) and two or three susceptible cultivars of V. vinifera ('Black Queen', 'Carolina Black Rose', and/or 'Italia'; female parents). F 1 hybrid seedlings were evaluated for downy mildew and anthracnose resistance using a detached/excised leaf assay. For both diseases, the general combining Inheritance of grape downy mildew and anthracnose resistance ability (GCA) variance among male parents was significant, while the variance of GCA among females and the specific combining ability (SCA) variance were not significant, indicating the prevalence of additive over non-additive gene actions. The estimated narrow sense heritabilities of downy mildew and anthracnose resistance were 55.6 and 79.2%, respectively, suggesting that downy mildew/anthracnose resistance gene(s) were highly heritable. The 'Carolina Black Rose x NY65.0550.04' cross combination is recommended for future use.

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“…The SCAR marker OPB 151247 was linked to the anthracnose resistance locus in grape (Kim et al, 2008 ). Three resistance gene analog‐single‐strand conformation polymorphism (RGA–SSCP) markers were subsequently found with significant correlation with anthracnose resistance using the interspecific hybrid NY65.0550.04 as a resistance source (Tantasawat et al, 2012 ). The markers from different resistance sources seem to have limitations of application to some extent.…”

Section: Genetics and Host Resistancementioning

confidence: 99%