Association of Seminal Plasma Total Antioxidant Capacity and Malondialdehyde Levels With Sperm Parameters in Infertile Men With Varicocele

Salimi, Saeedeh; Fazeli, Faramarz; Khosravi, Paria; Nabizadeh, Sima

doi:10.17795/ajmb.33540

Cited by 4 publications

(4 citation statements)

References 31 publications

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“…With this in mind, the result of total antioxidant capacity (TAC) analysis in the current study revealed significantly lower activity in asthenozoospermic. Our result was similar to Salimi et al [39], and Hosen et al [40] studies established that the total antioxidant capacity value in infertile men was lower than that in fertile men. In addition, Riaz et al .…”

Section: Discussionsupporting

confidence: 93%

Assessment of correlation between asthenozoospermia and mitochondrial DNA mutations in Egyptian infertile men

Elrahman

Makawy

Hassanane

et al. 2021

Journal of Genetic Engineering and Biotechnology

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Background Asthenozoospermia is a chief reason for male seminal pathologies with an impression of around 19% of infertile patients. Spermatozoa mitochondrial DNA variations seem to link with low sperm motility. The objective of the study was to assess the relation between mitochondrial mutations and male sterility, especially in asthenozoospermia. The patient semen samples were investigated by studying the sperm physical characters; motility, viability, and morphological parameters were then classified into normozoospermia and asthenozoospermia. In addition, the level of malondialdehyde (MDA) as a bio-indicator of lipid peroxidation, seminal fructose, and total antioxidant capacity (TAC) were estimated. For molecular analysis, DNA from the semen samples was extracted using a DNA extraction kit. ND1, ND2, and ATPase6 genes were amplified by using a specific primer. After the purification procedure, each PCR product was sequenced to identify the single nucleotide polymorphisms (SNPs) in selected genes. Results A significant negative correlation between seminal plasma malondialdehyde levels and sperm motility was detected. Meanwhile, TAC analysis revealed significantly lower activity (p ≤ 0.05) in the sample of asthenozoospermic than in normozoospermic men. As regards the seminal plasma fructose, there was no significant difference in the fructose level of normozoospermia and asthenozoospermia cases. At the molecular level, 31 diverse nucleotide substitutions were recognized in mitochondrial DNA. Only ten (10) mutations led to amino acid transformation: four have deleterious effects, four are benign, and the other two have conflicting effectiveness. Conclusions This study is the first in Egypt that is concerned with studying the relationship between the mitochondrial DNA mutations in human spermatozoa of asthenozoospermic patients and fertility. The results displayed scientific indications evidenced that there is an association between mitochondrial mutations and male infertility.

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Section: Discussionsupporting

confidence: 93%

Assessment of correlation between asthenozoospermia and mitochondrial DNA mutations in Egyptian infertile men

Elrahman

Makawy

Hassanane

et al. 2021

Journal of Genetic Engineering and Biotechnology

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“…The dosedependent increase in TAC was strongly associated with the progressive rise in sperm cell viability and the decreases in apoptosis, necrosis, and structural abnormalities. Consistent with these findings, Salimi, et al [44] reported positive correlations between TAC and both sperm motility and normal sperm morphology, while Pahune, et al [45] observed positive correlations between TAC and multiple seminogram parameters including sperm concentration, sperm motility, and normal sperm morphology. Collectively, these findings suggest that an imbalance between TAC and ROS production is a major contributor to impaired sperm function following cryopreservation [46].…”

Section: Discussionmentioning

confidence: 64%

Antioxidant and Antiapoptotic Effects of a Turraea fischeri Leaf Extract on Cryopreserved Goat Sperm

Hassan

Khalil

Hassan

et al. 2021

Animals

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This study evaluated the efficacy of Turraea fischeri leaf extract for maintaining the viability of cryopreserved goat sperm. Ejaculated semen was collected from 5 mature Baladi bucks (50–60 kg, 2–4 years of age) and those samples with mass motility ≥ 70% and sperm concentration ≥ 2.5 × 109/mL were selected, pooled, and divided into 4 aliquots. Each aliquot was diluted in Tris-citric-soybean lecithin extender containing a different concentration of T. fischeri leaf extract (0, 125, 250, or 375 µg/mL). Treated semen samples were cooled to 5 °C, transferred to 0.25-mL French straws, and stored in liquid nitrogen (LN2) at −196 °C. After thawing, membrane integrity was examined by transmission electron microscopy, apoptotic activity by Annexin/propidium iodide staining and flow cytometry, and both enzyme activities and antioxidant capacity by spectroscopic assays. The leaf extract at 375 µg/mL significantly improved semen quality as indicated by enhanced total antioxidant capacity, reduced H2O2 concentration, a greater proportion of structurally intact motile sperm, and concomitant reductions in apoptosis and necrosis. The extract also significantly increased the proportion of sperm with a contiguous plasma membrane and intact acrosome (p < 0.05). Furthermore, LC-MS revealed numerous secondary metabolites in the extract that may contribute to sperm cryopreservation.

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“…The total antioxidant capacity (TAC) of seminal plasma was measured using the ferric reducing ability of plasma (FRAP) method as described by Salimi et al (2016). This method is based on the ability of the antioxidants in the seminal plasma to reduce ferric-tripyridyltriazine (Fe 3þ -TPTZ) to a ferrous form (Fe 2þ ).…”

Section: Total Antioxidant Capacity Of Seminal Plasmamentioning

confidence: 99%

Effects of moderate COVID-19 infection on semen oxidative status and parameters 14 and 120 days after diagnosis

Falahieh

Zarabadipour

Mirani

et al. 2021

Reprod. Fertil. Dev.

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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the virus causing coronavirus disease 2019 . Because COVID-19 is a multisystem infection, there are some concerns regarding its possible effects on male fertility. This study aimed to investigate the effects of COVID-19 on semen oxidative status and parameters 14 and 120 days after diagnosis in patients presenting with moderate infection (defined as respiratory symptoms, with or without fever, with S p O 2 ,93% and .90% and lung involvement ,50%). Semen samples were obtained from 20 participants at two time points: the first sample on Day 14 and the second on Day 120 after diagnosis. Semen parameters (sperm concentration, motility, morphology, and viability) were evaluated, as were levels of seminal reactive oxygen species (ROS), malondialdehyde (MDA), total antioxidant capacity (TAC) and sperm DNA fragmentation. Semen parameters, including sperm motility and DNA integrity, improved at 120 days after the COVID-19 diagnosis relative to values at 14 days. In addition, ROS and MDA levels were significantly reduced in patients 120 days after infection, and TAC increased at 120 days compared with 14 days (during the acute stage of infection). In conclusion, the present study shows that the detrimental effects of COVID-19 on sperm properties caused by oxidative stress decrease up to Day 120 after diagnosis.

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Association of Seminal Plasma Total Antioxidant Capacity and Malondialdehyde Levels With Sperm Parameters in Infertile Men With Varicocele

Abstract: Background: Varicocele is one of the most common reasons for male infertility and could impair spermatogenesis through mechanisms that are not well known. Recently, oxidative stress has been introduced as a major reason for male infertility caused by varicocele.

Cited by 4 publications

References 31 publications

Assessment of correlation between asthenozoospermia and mitochondrial DNA mutations in Egyptian infertile men

Assessment of correlation between asthenozoospermia and mitochondrial DNA mutations in Egyptian infertile men

Antioxidant and Antiapoptotic Effects of a Turraea fischeri Leaf Extract on Cryopreserved Goat Sperm

Effects of moderate COVID-19 infection on semen oxidative status and parameters 14 and 120 days after diagnosis

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