Association of T1740C polymorphism of L-FABP with meat quality traits in Junmu No. 1 white swine

Hui

J Animal Sci Biotechnol

et al. 2016

BackgroundTo explore the relationship between the heart-type fatty acid binding protein (H-FABP) gene and intramuscular fat (IMF), a polymorphism of the second exon of the H-FABP gene was investigated in 60 Three-yellow chickens (TYCs) and 60 Hetian-black chickens (HTBCs).ResultsThe IMF contents of the cardiac, chest and leg muscles in HTBC were increased compared with TYC. Both TYC and HTBC populations exhibited Hardy-Weinberg Equilibrium (HWE) according to the χ2 test. Three variations of the two birds were detected, namely, G939A, G982A and C1014T. HTBCs with the TT genotypes exhibit increased IMF content in the chest muscles compared with the TC genotype. Thus, the G982A site could be considered a genetic marker for selecting increased IMF content in the chest muscles of HTBC. The correlation coefficients revealed that H-FABP mRNA expression was negatively correlated with the IMF content in the cardiac, chest and leg muscles of HTBC and in the cardiac and chest muscles of TYC. The relative mRNA expression of H-FABP was reduced in the cardiac and leg muscles of HTBC compared with TYC, but this difference was not observed at the protein level, as assessed by Western blot analysis.ConclusionsThese findings offer essential data that can be useful in the breeding program of HTBC and future research exploring the role of H-FABP in IMF deposition and regulation in chickens.

Section: Discussionmentioning

confidence: 99%

Association of H-FABP gene polymorphisms with intramuscular fat content in Three-yellow chickens and Hetian-black chickens

Hui

J Animal Sci Biotechnol

et al. 2016

“…Polymorphisms of the A-FABP gene are found to be prognostic biomarkers in patients with acute coronary syndrome (Reiser et al, 2015) and type 2 diabetes mellitus (Alqahtani et al, 2016). They are usually researched as candidate genes that affect the deposition of intramuscular fat in pigs (Zhang et al, 2013), cattle (Jurie et al, 2007), chickens (Zhou et al, 2018), ducks (Saez et al, 2009), goats (Wang, Geng, Ji, & Chang, 2012) and sheep (Wang et al, 2014). The adipocyte fatty acid-binding protein (A-FABP) in the superfamily of lipid-binding proteins (Wang et al, 2006) plays an important role in the lipid deposition of muscles and homeostasis in adipocytes (Migdał et al, 2018).…”

mentioning

confidence: 99%

Association of A‐FABP gene polymorphism and mRNA expression with intramuscular fat content (IMF) in Baicheng‐You chicken

Animal Physiology Nutrition

Liu

Hang

et al. 2019

This study aims to assess the association of polymorphisms and mRNA expression of adipocyte‐type fatty acid‐binding protein (A‐FABP) with intramuscular fat (IMF) in the breast muscle (BM) and leg muscle (LM) of Baicheng‐You chickens (BYCs). A total of 180 chickens, including sixty black Baicheng‐You chickens (BBYCs), sixty silky Baicheng‐You chickens (SBYCs) and sixty white Baicheng‐You chickens (WBYCs), were reared from 1 to 120 day. A polymerase chain reaction–single‐strand conformation polymorphism strategy (PCR‐SSCP) was used to detect the polymorphism of the A‐FABP gene in the first exon, and the C51T silent mutational site was found. The IMF content with the AA genotype was significantly higher than that with the AG genotype (p = 0.0473) in the LM of WBYC. Thus, this site could be taken as a molecular marker in selecting a higher IMF content of LM in WBYC. A‐FABP gene mRNA expression in the BM and LM of BYCs was detected, and a significant positive correlation was observed in the LM of WBYC. These findings provide fundamental data that might be useful in further study of the role of the A‐FABP gene in IMF content and fatty metabolism in chickens.

“…L-FABP polymorphisms were reported to be related to fatty acid such as C16:0, C18:3, and total IMF in chest muscle, where CC genotype had higher contents when compared to CT and TT genotypes (He et al, 2011). The effect on marbling in white swine showed that the polymorphism had a significant effect in genotype CC where it had more marbling than TC, and TC had more marbling than TT; besides, the effect on intramuscular fat content was also polymorphic, it showed that L-FABP might be a candidate gene associated with meat quality traits (Zhang et al, 2013). However, this gene and its effect on the quality of duck carcasses has never been explored in Indonesian local ducks.…”

Section: Introductionmentioning

confidence: 93%

Polymorphism of L-FABP (SNP g. 1593 C>T) Gene and Its Association with Fatty Acid Composition, Carcass, and Meat Quality in Cihateup Duck

Mentari¹,

Gunawan²,

Noor³

2020

Trop. Anim. Sci. J.

Fatty acid composition, carcass, and meat quality are quantitative traits which are controlled by several genes or polygenic. One of the genes that plays important role in the fatty acid in meat quality and fatty acid composition is liver-type fatty acid-binding protein (L-FABP). The aims of this study were to identify single nucleotide polymorphism (SNP) of liver-fatty acid binding protein (L-FABP) gene and its association with fatty acid, carcass, and meat quality traits in cihateup duck. Cihateup ducks were originated from Tasikmalaya, West Java, Indonesia. The study used a total of 98 ducks aged of 12 weeks old with the average body size of 1.4±0.12 kg for PCR-RFLP analyses and 76 ducks for the association analyses. Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) was used to identify SNP g. 1593 (C>T) of L-FABP gene. The associations of L-FABP genotypes with fatty acid, carcass, and meat quality traits were performed using T-test procedures. The result showed that the SNP of L-FABP gene was polymorphic with three genotypes (CC, CT, and TT). The Chi-square test revealed that the locus of L-FABP (g. 1593 C>T) was in Hardy -Weinberg equilibrium. L-FABP gene was significantly associated (p<0.01) with the carcass portions, providing neck and percentage of neck as well as significantly associated (p<0.05) with saturated fatty acids, i.e., lauric acid (C 12:0) and palmitic acid (C 16:0); polyunsaturated fatty acid, i.e., eicosadienoic acid (C 20:2); carcass portions, i.e. neck and percentage of neck. The SNP g. 1593 (C>T) of L-FABP gene may be a useful marker for selecting and producing duck meat having desirable fatty acids and carcass and meat quality.