Association of the bovine growth hormone gene with Holstein cattle reproductive parameters

Abstract

Objective. To determine the association of intron 3 polymorphism of the bovine growth hormone (BGH) gene, with age at first service, first birth, first postpartum service and second birth in a population of Holstein cows in the state of Antioquia. Materials and methods. The study was conducted on 408 Holstein cows in 8 herds. Genotyping was performed using PCR-RFLP technique with DNA extracted from peripheral blood by the salting out technique. The Phenotypic information used was collected for 4 years, from a dairy production control program. To determine the association between characteristics and gene polymorphism, parametric statistical analyzes were performed as generalized linear models and linear regression analysis. Results. Allele frequency (positive) was 0.91 and was 0.09 for (negative) allele. Genotypic frequencies were 0.77, 0.2 and 0.03 for (positive/positive), (positive/negative) and (negative/negative) respectively. There were significant differences between the mean of age at first service, age at first birth, age at first postpartum service and age at second birth. For all of the (negative/negative) genotype characteristics there were greater ages for each event. Conclusions. These results suggest that intron-3 polymorphism of the BGH gene is associated with reproductive traits, facilitating the selection of individuals with favorable genotypes for use in breeding programs.

Assessment of growth hormone gene polymorphism effects on reproductive traits in Holstein dairy cattle in Tunisia

“…To date, few studies have investigated the effect of GH-MspI polymorphism on reproductive traits of bulls, and the results of these studies have reported a significant association between GH-MspI polymorphisms and the following sperm quality traits in bulls: fresh sperm motility, sperm concentration, minor and major defects, scrotal circumference and testicular growth after puberty (Lechniak et al, 2002;Una-nian et al, 2002;Gorbani et al, 2009); the reproductive traits of beef cattle have also been studied (Anggraeni et al, 2017). Arango et al (2014) reported that MspI polymorphism is associated with age at first service, first birth, first postpartum service and second birth. Furthermore, the MspI (+/+) genotype had the greatest effect on the age at first service and was considered the optimal genotype for reducing age at first service, first birth, first postpartum service and second birth.…”

Identification of novel single nucleotide polymorphisms in the growth hormone ( GH ) gene in Anatolian water buffalo ( Bubalus bubalis ) populations in Turkey

“…However, especially because of extensive production system in water buffalo in Turkey, it was not possible to have data for milk or meat yield. Arango et al (2014) identified the significant association of genotypes at the BGH-MspI locus with age at first service, first postpartum service, and first and second births in Holstein cows in the Antioquia province. Amiri et al (2018) showed a significant effect of the GH-AluI on the calving interval and the days open.…”

Genotype analysis of partial growth hormone gene (GH891│MspI) in Pesisir cattle and Simmental-Pesisir crossbred cattle

“…Similarly Beauchemin et al (2006) has reported that effects of MspI polymorphism were not found in Brahman cattle and Nellore cattle population (Cardoso et al, 2014). On the contrary, Unanian et al (2000) reported that MspI polymorphism effects on the growth traits in Nellore cattle population and describes a significant effect on the average daily gain (ADG) from weaning at 15 months with DD genotype (equivalent with MspI-/-) and weight at first estrus and first calving in heifers in Holstein cattle (Arango et al, 2014). For the other SNP of GH in cattle, Reis et al (2001) reported that genotype LV of Portuguese cattle breeds have weight live higher than LL and VV in mature weight.…”