Association of the
            <i>r</i>
            IIA Protein with the Bacterial Membrane

Ennis, Herbert L.; Kievitt, K D

doi:10.1073/pnas.70.5.1468

Cited by 36 publications

(20 citation statements)

References 28 publications

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“…Possible interaction of the DD gene products with the host membrane. The DD genes 39, 52, and 60 are located in that region of the T4 genetic map which contains several other genes which have been shown either to modify the host membrane, such as rIIA (10) and rIIB (22, 29), or to have membrane related functions such as the ac gene (8,24) and gene t (16).…”

Section: Discussionmentioning

confidence: 99%

“…The burst sizes in these two cases were Total incorporation of [methyl-3H]thymidine into DNA. Samples were taken at 5,10,15,25,35,45,60, 75, and 100 min after infection of E. coli S/6. Infection was carried out with am mutants representative 6f the four DD genes and with gene 41 mutant amN57 and wild type as controls.…”

Section: Incorporation Of [Methyl-3h]thymidine Intomentioning

confidence: 99%

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The DNA-Delay Mutants of Bacteriophage T4

Mufti

Bernstein

1974

J Virol

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observations, taken together with those of others, suggest that these products are necessary for normal DNA synthesis and that they interact with each other and a bacterial component probably at the membrane. MATERIALS AND METHODS Phage and bacterial strains. All T4 phage mutants used in this study came from the California Institute of Technology collection under the care of W. Wood. The designations of these mutants are indicated in the gene maps shown in Fig. 1. Three E. coli strains lacking an am suppressor S/6, 594, and B/5, as well as two strains containing an am suppressor, CR63 and 011', were used. E. coli 594 (4) and CR63 (1) were derived from E. coli K12, whereas S/6, B/5, and 011' were derived from E. coli B (11, 31). Media. H-broth was used for growth and suspension of phage and bacteria. EHA bottom and top agar were used for plaque assays or for plating bacteria (25). M9 medium (6) was used for growth of infected cells in DNA-labeling experiments. Preparation of phage stocks. The phage stocks used were clonally derived from single plaques. High titer phage stocks were prepared by a plate lysate method adapted from that described by Edgar (9). Phage lysates used for [-'H]thymidine incorporation experiments were prepared on M9 plates or in M9 liquid medium without thymidine. Preparation of bacterial plating cultures. Fresh log phase cultures of bacteria were used for phage assays. These were prepared by diluting a fresh overnight culture 1:1,000 in H-broth and growing the 860

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Section: Discussionmentioning

confidence: 99%

Section: Incorporation Of [Methyl-3h]thymidine Intomentioning

confidence: 99%

The DNA-Delay Mutants of Bacteriophage T4

Mufti

Bernstein

1974

J Virol

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“…It is, however, partially functional in its interaction with the replication complex. ( Since the rII proteins are bound to the membrane (34)(35)(36) and to DNA (37), the rII-suppression of L171 implies that the conversion of "joint" into "recombinant" molecules occurs at the membrane. This implication is strengthened by the suppression of the rII-plaque morphology by some gene 32 (and ligase) mutations.…”

Section: Discussionmentioning

confidence: 99%

Genetic evidence for an additional function of phage T4 gene 32 protein: interaction with ligase.

Mosig¹,

Breschkin²

1975

Proc. Natl. Acad. Sci. U.S.A.

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Gene 32 of bacteriophage T4 is essential for DNA replication, recombination, and repair. In an attempt to clarify the role of the corresponding gene product, we have looked for mutations that specifically inactivate one but not all of its functions and for compensating suppressor mutations in other genes. Here we describe a gene 32 ts mutant that does not produce progeny, but in contrast to (6,(9)(10)(11). It is produced in large amounts after T4 infection and is limiting for phage production (12). This could mean that (i) DNA replication, recombination, and repair are interdependent, and although gene 32 protein is directly involved in only one of these processes, it indirectly affects the other two; (ii) the protein is directly involved in all three processes and serves the same function in each; or (iii) the protein serves a direct but different function in each of the three processes.We have found that different gene 32 ts mutants have quite different phenotypes, and that at least three, L171 being one of them, are partially suppressed by rII mutations (ref.

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“…infection. Altematively, the late function may increase membrane fragility directly by modifying the membrane; changes in membrane composition after infection have been reported (11,12,15,21,34,46,51). Furrow and Pizer (15) have found that after T4 infection the relative rates of 32p; incorporation into phosphatidylglycerol and phosphatidylethanolamine were altered.…”

Section: -mentioning

confidence: 99%

Late effect of bacteriophage T4D on the permeability barrier of Escherichia coli

Thompson¹,

Wiberg²

1978

J Virol

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Cold centrifugation of lysis-inhibited Escherichia coli B infected with wildtype T4D results in extensive lysis beginning around 20 min after infection at 370C. Infection with an e mutant, which fails to make lysozyme, prevents lysis, but does not prevent a marked loss of K+ and Mg3+. The t gene product, thought to disrupt the cytoplasmic membrane in natural lysis, is not required for this handling-induced cation loss or lysis. Three lines of evidence argue that late protein synthesis is required to develop this potential for cation loss; the potential does not develop in infections by: (i) mutants defective in DNA synthesis, (ii) mutants defective in gene 55, and (iii) wild-type T4 when chloramphenicol is added at 6 min after infection. All late mutants examined, which are blocked in the major pathways of morphogenesis, do not prevent development of the potential. The evidence argues for a new, late effect of T4 infection on the cytoplasmic membrane.

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Association of the r IIA Protein with the Bacterial Membrane

Cited by 36 publications

References 28 publications

The DNA-Delay Mutants of Bacteriophage T4

The DNA-Delay Mutants of Bacteriophage T4

Genetic evidence for an additional function of phage T4 gene 32 protein: interaction with ligase.

Late effect of bacteriophage T4D on the permeability barrier of Escherichia coli

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